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EMAGE:31382

Psma3 proteasome (prosome, macropain) subunit, alpha type 3 ( MGI:104883)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382
euxassay_003148_01 euxassay_003148_02 euxassay_003148_03 euxassay_003148_04 euxassay_003148_05
EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382
euxassay_003148_06 euxassay_003148_07 euxassay_003148_08 euxassay_003148_09 euxassay_003148_10
EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382
euxassay_003148_11 euxassay_003148_12 euxassay_003148_13 euxassay_003148_14 euxassay_003148_15
EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382
euxassay_003148_16 euxassay_003148_17 euxassay_003148_18 euxassay_003148_19 euxassay_003148_20
EMAGE:31382 EMAGE:31382 EMAGE:31382 EMAGE:31382
euxassay_003148_21 euxassay_003148_22 euxassay_003148_23 euxassay_003148_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
ventral grey horn
strong strong
regionalstrong expression: see section 12 13 18 19 moderate expression: see section 15 16 17
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 08 20
axial musculature
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 22 23 24
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 11 12 13 14 19 20
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 22 23
submandibular gland primordium
strong strong
regionalstrong expression: see section 21 moderate expression: see section 08 09 10 11 18 19 20 22
medulla oblongata basal plate
strong strong
regionalstrong expression: see section 18 moderate expression: see section 10 11 17
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 19
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 13 14 15 16 17
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24
vibrissa
moderate moderate
regionalmoderate expression: see section 07 08 09 10 22 23 24
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 17 18
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 15 17 18 19
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 04 05 06 07 23 24
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4701
Entity Detected:Psma3, proteasome (prosome, macropain) subunit, alpha type 3 ( MGI:104883)
Sequence:sense strand is shown

>T4701
GCCGGGTCAGCACCATGAGCTCCATCGGCACTGGGTATGACTTGTCAGCCTCTACGTTCTCTCCTGATGG
AAGAGTTTTCCAAGTTGAATATGCCATGAAGGCTGTGGAAAACAGTAGTACAGCTATTGGGATCAGATGT
AAAGATGGTGTTGTGTTTGGGGTAGAAAAACTAGTCCTTTCTAAACTTTATGAAGAAGGCTCCAATAAAC
GTCTTTTTAATGTTGATCGACATGTTGGAATGGCAGTTGCAGGTTTGCTGGCAGATGCTCGTTCATTAGC
AGACATAGCGAGAGAAGAAGCATCCAACTTTAGGTCCAACTTCGGCTATAACATTCCTCTAAAACATCTT
GCAGACAGAGTGGCCATGTATGTACACGCCTATACACTCTACAGCGCTGTTAGACCTTTTGGCTGCAGTT
TCATGTTGGGGTCTTACAGTGCGAATGATGGTGCACAGCTCTATATGATCGACCCATCAGGTGTTTCATA
TGGTTACTGGGGCTGTGCGATTGGCAAAGCCAGGCAGGCTGCAAAGACAGAGATAGAAAAGCTTCAGATG
AAGGAAATGACTTGCCGTGATGTAGTTAAAGAAGTTGCAAAAATAATTTACATAGTACACGATGAAGTTA
AAGATAAAGCTTTTGAACTGGAGCTCAGCTGGGTTGGTGAATTAACTAAAGGAAGACATGAAATTGTTCC
CAAAGACATAAGAGAGGAAGCAGAGAAATATGCCAAGGAATCTTTGAAGGAAGAAGATGAATCAGATGAT
GACAATATGTAATATTTTCTTCCCCATCCGCTGGTTTACATTTCTGTAACAGTTGCATATGGCTAGCACC
ATGGACTATATATACCCACTGACCGATTTTCATTAAATTTTGTCTTATAAACATAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5030475 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030475 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003148 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS