EMAGE mouse gene expression spatial database

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EMAGE:31393

Tinf2 ( MGI:107246)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002058_01	euxassay_002058_02	euxassay_002058_03	euxassay_002058_04	euxassay_002058_05

euxassay_002058_06	euxassay_002058_07	euxassay_002058_08	euxassay_002058_09	euxassay_002058_10

euxassay_002058_11	euxassay_002058_12	euxassay_002058_13	euxassay_002058_14	euxassay_002058_15

euxassay_002058_16	euxassay_002058_17	euxassay_002058_18	euxassay_002058_19	euxassay_002058_20

euxassay_002058_21	euxassay_002058_22	euxassay_002058_23	euxassay_002058_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 17 moderate expression: see section 08 09 15 16 18

thymus primordium

moderate

homogeneous

moderate expression: see section 09 10 weak expression: see section 11 12 13

renal cortex

moderate

regional

moderate expression: see section 04 05 06 07 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 08 09 19 20 21 weak expression: see section 07

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4622
Entity Detected:	Tinf2, ( MGI:107246)
Sequence:	sense strand is shown >T4622 CCACGCGTCCGTGAGAGGAGACTGCGGGGTTCCCGTGGCTCCGCGAAATGGGCCCAGCTGGTCCGCCGAC CTTCCGGTGCAGATGAGAGAAGCGGGGCTCCGCCTACGGGAGCGTCCGGGAAGCCTGGGTTGAACGAAAG GAGGAAAGGGCGCCCGCGGGCCATGGCCCCACCTCCAGGGGTAGGTCCCGCGTCTCTGCGGTTTGCTGCC GCCGCCAGCTGGCTGGTAGTCCGCCGTCGCCGCGTAGAGCACTTCCCGAAAGTGGTGGAGTTTCTGCAGT CCTTGCGCGCTGCTGCCCCCGGCTTGGTTTGCTACCGGCACCATGAACGCCTGTGTATGAGCCTAAAGGC CAAGGTGGTGGTGGAGCTGATCCTGCAGGCGCGACCCTGGGACCAGGTCCTGAATGCCCTGAAGCATCAC TTCCCAGCAGAGTCCAGAACAACAAAAGAAGACAGGAAGCTTTTGGAGGCACGGGAAAATTTTTGCTTGC TTGTGAAGCACCTGTCAGAGGACCCGCCTTCGAGCCTGCAGGAACTAGAACAAGACTATGGGGAATCCTT TCTAGTTGCCATGGAAAAGCTGTTGTTTGAATACTTGTGTCAGCTAGAGAAAGCACTGCCTCCAGTCAGA GCACAAGAGCTTCAGGATGCTCTAAGTTGGAGTCAGCCGGGCTCGTTCATCACTTCTTCGGTTGCTTTGC ACCAGTATGGTATGGACATGGGGTGGACATTTCCAGAGAGCTCTACTTCTGGCTCAGGGAATCTGATAGA GCCCATGGAAGAGAGTCCTCATCAGCAAACCAGGCCAGCATTCCACAGTCCTCTGCCTAAAGCTAAGCTT GGCCCTCACCAGCCAGCTTCACTGGAGCACCCAGAACACTTAGCTGGCCACCGCTTTAATCTGGCCCCTT TGGGAAAGCGAAAATCCCGATCACATTGGACATCGGCAAAGGCGTGCCATAAAGAGCGGCCCACAGTCAT GCTACTCCCATTTAGGAACATGGGCTTACCAGCTCAAGACTTATCTAACCCTAAAAGCAGGGAAGAACCC GGTGCGGCCTCAGCAGCTTCTGTGGGAACAGAGCCAGTCTGCACGGAGGAGGCTAAGACTCCATCTCGGC CTTTGGGGAAAAGGGCATTAGAGGAGACCCCACCTGACTCACCGGCTGCAGAGCAGGAGAACAGTGTGAA TTGCGTGGATCCTCTAAGACACTCATCACCGCCTCTTACAGTCAAGAAACCAGTGCTGTCCCCAACCCCG TGTAGCTCTGTTATTACCATAGGGGACTTGGTTTTGGACTCTGATGAGGAAGAAAATAACCAGAAGGAAG GGAAGGAGTTTTTGAAAAACTACCAGAAGACGAAGTTTGACACCTATATCCCCATGTTTTGTGACTACAT CCCGTAGTGTCCTGCCTATTCCTTTCTGGCTACCTAAACGGTTCTCACTCAGGTCCTCTGGAATATTCTC TGCACTCTGTCCATCTTCTGCTGCAGCTCTGCATGCAATTTAGTGGAAAAGAAGAGGAAGTCCAGACTTG AGTTGCCTGGCTACATTGCTGAAAGCTTTGTAGCACTTGAAAATTAAATATTGATTTGTTTCACTATTGA TGGGAAGAAGGGGAATAAAGACTTGCATAGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5003804 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5003804 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002058 same experiment
	EMAGE:29250 same embryo
	EMAGE:30939 same embryo
	EMAGE:31395 same embryo
	EMAGE:31376 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source