EMAGE mouse gene expression spatial database

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EMAGE:31398

Acaa1b acetyl-Coenzyme A acyltransferase 1B ( MGI:3605455)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 weak expression: see section 14 15 16 17

lower jaw incisor

strong

regional

strong expression: see section 12 18 moderate expression: see section 13 17

upper jaw incisor

strong

regional

strong expression: see section 12 18 moderate expression: see section 13 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4922
Entity Detected:	Acaa1b, acetyl-Coenzyme A acyltransferase 1B ( MGI:3605455)
Sequence:	sense strand is shown >T4922 CCCACCTCCGCGATGCATCGGCTGCAGGTAGTGCTGGGTCACCTGGCCGGCCGGCCCGAGTCGAGCTCCG CGCTGCAAGCTGCGCCCTGCTCCGCTGGCTTCCTGCAGGCCTCGGCCTCCGACGTGGTGGTGGTGCACGG ACGGCGCACTCCCATTGGCCGCGCCAGCCGTGGCTGCTTCAAGGACACCACCCCCGACGAGCTTCTGTCG GCCGTGTTGACCGCGGTTCTCCAGGACGTGAAGCTAAAGCCTGAGCAGCTGGGAGACATCTCCGTGGGCA ATGTGCTTCAGCCCGGGGCTGGAGCCATTATGGCACGCATCGCCCAGTTCCTGAGCGGCATCCCAGAGAC TGTACCTTTGTCTACGGTCAACAGACAGTGTTCATCGGGACTGCAGGCAGTGGCCAACATTGCTGGTGGC ATCAGAAATGGGTCTTATGACATTGGCATGGCCTGTGGGGTGGAGTCCATGACCCTGTCCCAGAGAGGGA ACCATGGGAATATTTCTTCCCGCCTGCTGGAGAACGAGAAGGCCAGAGACTGCCTGATTCCTATGGGGAT AACTTCGGAGAATGTGGCTGAGCGGTTTGGCGTTTCACGGCAGAAGCAGGATGCCTTTGCGCTGGCCTCC CAGCAGAAGGCAGCAAGCGCCCAGAGCAGAGGATGCTTCCATGCTGAGATTGTGCCTGTGACAACCACTG TCCTGAATGACAAGGGTGACAAGAAAACCATCACTGTGTCTCAGGATGAGGGTGTCCGCCCCAGCACCAC CATGCAGGGCCTGGCCAAGCTGAAGCCTGCCTTCAAGGATGGAGGCTCTACCACGGCTGGAAACTCCAGT CAGGTGAGTGATGGAGCAGCTGCCGTCCTGCTGGCTCGGAGGTCCAAGGCTGAAGAACTGGGCCTCCCCA TCCTTGGGGTCCTGAGGTCCTATGCAGTGGTCGGGGTCCCTCCTGACGTCATGGGCATCGGACCTGCCTA TGCCATCCCTGCAGCCTTGCAGAAAGCAGGGCTGACTGTGAATGACATAGACATCTTTGAGATCAATGAG GCCTTTGCAAGTCAGGCCGTCTACTGTGTGGAGAAGTTAGGAATTCCTGCAGAGAAGGTGAACCCGCTGG GGGGTGCAATAGCCCTGGGCCACCCCCTGGGCTGCACGGGAGCAAGGCAGGTTGTCACGCTACTCAATGA ACTGAAGCGCCGTGGCAGACGGGCTTACGGCGTGGTATCCATGTGCATCGGGACTGGGATGGGAGCTGCT GCGGTCTTTGAATACCCTGGGAACTGAGGCCTGGCTGCAGGCGGCACAACCCAGAGAGTGCCACAGTGGT GTCCAGAGAGGGACGCTACAGAAGCCGTCTGCGTGGGACACTCAGCAGTGGAGGGGTGTGTCACAGCACT TTAATTTAGAAAATGTAATTGCAGCATGGGTACACGTGGGCACTGGGGCATCCAATCCGGTTCTCTCGGT CGAACCAAAGACAGTGCATGCTATGCTTGACCACAGGACAACATTCATTTCTGTGGGACGGTGCCTTCAT GAATGGTGGATGTGATGGGCACAGTAAACGTGGGTGAACTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5123887 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5123887 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003143 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source