EMAGE mouse gene expression spatial database

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EMAGE:31411

Mknk1 MAP kinase-interacting serine/threonine kinase 1 ( MGI:894316)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003111_01	euxassay_003111_02	euxassay_003111_03	euxassay_003111_04	euxassay_003111_05

euxassay_003111_06	euxassay_003111_07	euxassay_003111_08	euxassay_003111_09	euxassay_003111_10

euxassay_003111_11	euxassay_003111_12	euxassay_003111_13	euxassay_003111_14	euxassay_003111_15

euxassay_003111_16	euxassay_003111_17	euxassay_003111_18	euxassay_003111_19	euxassay_003111_20

euxassay_003111_21	euxassay_003111_22	euxassay_003111_23	euxassay_003111_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 16 17 18

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4697
Entity Detected:	Mknk1, MAP kinase-interacting serine/threonine kinase 1 ( MGI:894316)
Sequence:	sense strand is shown >T4697 CCACGCGTCCGCGCGGCCGTTCCCGCGGTGGTCAACTAAGATCTCTGTGCTGGAGGAGATGAAGCAGAAG CAGGTGGCAGACTAAAGGACACTGGCTGGACAGAGACCAGCAGATAACTCCCTGTGGCTGGAAGACACCA GTCTTGGGATTCCTCTGAGACTCCAAGTTAAAGAACCTTGTGGAGATGGGCAGCAGTGAGCCCCTTCCCA TCGTGGATTCTGACAAGAGGAGGAAGAAGAAGCGTAAGACCCGGGCCACCGACTCTCTGCCAGGAAAGTT TGAAGATGTGTACCAGCTGACCTCGGAATTGCTGGGAGAAGGAGCCTATGCCAAAGTCCAGGGTGCTGTG AACCTACAGAGTGGGAAGGAGTATGCTGTCAAAATCATCGAGAAGCAAGCCGGGCACAGTCGAAGTCGAG TGTTCCGTGAGGTGGAGACACTGTATCAGTGTCAAGGGAACAGGAACATTTTGGAGCTGATTGAATTCTT TGAAGATGACACACGGTTTTACTTGGTCTTTGAGAAATTGCAAGGAGGCTCCATCCTAGCACACATCCAG AAGCGGAAGCACTTCAATGAGCGAGAAGCCAGTAGAGTGGTGCGGGACGTCGCCACTGCCCTTGACTTCC TGCACACTAAAGGCATTGCTCACCGTGATCTGAAGCCAGAAAACATACTGTGTGAATCTCCAGAAAAGGT GTCTCCGGTGAAAATTTGTGACTTTGACTTGGGCAGTGGGGTAAAGTTGAACAACTCCTGCACTCCCATA ACCACGCCAGAGCTGACTACTCCATGCGGCTCTGCAGAGTATATGGCACCTGAGGTGGTGGAGGTCTTTA GGGACGAGGCTACTTTCTATGACAAGCGCTGTGACCTGTGGAGCCTGGGTGTGGTCCTCTACATCATGCT GAGTGGCTACCCCCCCTTTGTAGGTCACTGTGGGGCTGACTGTGGCTGGGACCGGGGAGAGGTATGCAGG ATGTGCCAGAACAAGCTGTTTGAGAGCATCCAGGAAGGCAAATACGAGTTTCCTGACAAAGACTGGGCTC ACATCTCCAACGAGGCCAAAGACCTCATCTCTAAGCTCCTGGTTCGAGATGCGAAGCAAAGACTCAGTGC TGCCCAGGTTCTGCAGCATCCATGGGTACAAGGGCAAGCTCCAGAAAGGGGACTCCCCACGCCGCAAGTC CTTCAGAGAAACAGCAGCACCATGGACTTGACTCTCTTCGCAGCTGAGGCCATTGCCCTGAACCGCCAGC TGTCTCAGCATGAGGAGAATGAACTGGCCGAGGAACAGGAGGCCCTAGCTGAGGGCCTCTGCTCCATGAA GCTGTCCCCTCCATCCAAATCTCGCCTGGCTCGAAGGCGAGCCCTGGCCCAGGCTGGCCGAAGCCGAGAT GCAAACCCATGTTTGACACCGGCAGGGCTCTGAACCACTGCAGGCACTCCTTGTAGGCCTGTCCCAGCGG TGCCCCCTGAAAACCTGTAAGGTCAAAGTGTGAAGTAGGCAGAGGGACCTGCCCTGCAGCCCCTGCAGGC CTTTACCATCCACAAAGGCTCCCGGGTTCCCACCCATCCCCCATTTCCCTGGAGTCTTCAAAGGAGAAAG CTTTTTCCAAAGGGGTTGTCTTTGAAAAGGAAAGCAATCACTTGTCACTTTGCATAATCGCCTGCAGCAG GACCGTCTCTGCCGGGCTCCAAAGGCTGCTGATGTGCAGATCCCGGATCCAGCTTGCGGTTGGGGCTGCA GGGGCTGCAGCCTTCAGGGAGTCCAGCTTCCAGGAGCTGACAGATCTGCGGACGTGCATGCCTCTTCCCT GCACTGTCACCTCCTTCTGGCGATCCTCCCACCTTCCTCTGTCCTACAGACGTCCTCTCTGCCTGTCCTC TCCCTGGCTGAGCAAAGCCATCCCCTCAATTCAGGAAGGGCAGGGAACCAGATCAGTCTTCCGGTCTGTA ACACAGAGAAGCACATAATCTTTCTTTGCCGTGACCGAAATGTTCCTCATTTATCATCCTCTTCCATGTT TGGGATTGCTGCTAAAGTCAGTATTATTTCTTTTTAAAAATATATTTGCTTATTTAAACCATGTTCTTCC AGCAATCATAGAACTTCAAACTTGCACTGCAAGCCCGTAGATATTCCCCACGCGGATATCCACGCACTTA GGTTTTAATCCTCTCCCCGACAGTCTCAACTGATTTTAAGCTGTTACTATGATGAAAACGTTAGAGACTG GTGTCTTTGCATCTCTGTCTGCCGCTGCTGCCTCCACAAACCCAGGGTAGTCAGACTGCTGTGTCTGAGA GGAAGCCCTGTGCCCTGCTATGAGGAATGCTGCGCCCTCTGCCCCAGACATGAAGGCACAGCCACTGGAA AATAAGCTGTGGAGTCACGTAAAACAGATTTGCATATATGACATACAACATTAGTCAACCACAATCACCA AAATTCTCTCATAACTTGTGCTTGTTTTAATAAATAATTTAAAATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030044 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030044 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003111 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source