EMAGE mouse gene expression spatial database

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EMAGE:31412

A530089I17Rik ( MGI:2143585)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000085_01	euxassay_000085_02	euxassay_000085_03	euxassay_000085_04	euxassay_000085_05

euxassay_000085_06	euxassay_000085_07	euxassay_000085_08	euxassay_000085_09	euxassay_000085_10

euxassay_000085_11	euxassay_000085_12	euxassay_000085_13	euxassay_000085_14	euxassay_000085_15

euxassay_000085_16	euxassay_000085_17	euxassay_000085_18	euxassay_000085_19	euxassay_000085_20

euxassay_000085_22	euxassay_000085_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 11 17 18 weak expression: see section 12 16 19

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 11 12 15 17 18 20 22 weak expression: see section 13 19 21

vagus x ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 20

thymus primordium

moderate

regional

moderate expression: see section 11 14 15 weak expression: see section 12 13 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 17 18 weak expression: see section 19 20 21 22 23

vibrissa

weak

single cell

weak expression: see section 22

nucleus pulposus

moderate

spotted

moderate expression: see section 14

cerebral cortex

moderate

homogeneous

moderate expression: see section 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1723
Entity Detected:	A530089I17Rik, ( MGI:2143585)
Sequence:	sense strand is shown >T1723 TGGCCTCGAGCCAGATTCGGACGAGGATTAAAAACACTGTGCATCGTGACTGGCTATTGTATATCATTCA TGGGTTCTGCGGGCAGTCCAAGCTTTTGATCTATGGACGACCAGTGTATGTGACCCTAATAGCCAGGAGA TCCAGTAGGTTTGCTGGGACGCGCTTTCTCAAGAGAGGCGCAAACTGTGAGGGTGACGTTGCAAATGAGG TGGAGACCGAGCAGATCCTCTGTGATGCTTCTGTGATGTCTTTTACCGCAGGCAGTTACTCTTCTTACGT ACAAGTTAGAGGATCCGTTCCCTTATTCTGGTCCCAAGACATCTCAACTATGATGCCGAAACCACCCATT ACACTGGACCAGGCGGATCCCTTTGCACACGTGGCTGCTCTTCACTTTGACCAGATGCTCCAGAGGTTCG GCTCCCCCATCATCATCTTGAACTTAGTGAAGGAGAGGGAGAAGAGAAAGCACGAAAGGATCCTGAGTGA AGAGCTGGTGGCTGCGGTCACTTACCTGAACCAGTTCCTGCCACCGGAGCACACCATTGTCTACATCC
Notes:	The probe template was PCR amplified from IMAGE:479759 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:479759 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000085 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source