EMAGE mouse gene expression spatial database

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EMAGE:31413

Kcnip1 Kv channel-interacting protein 1 ( MGI:1917607)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003112_01	euxassay_003112_02	euxassay_003112_03	euxassay_003112_04	euxassay_003112_05

euxassay_003112_06	euxassay_003112_07	euxassay_003112_08	euxassay_003112_09	euxassay_003112_10

euxassay_003112_11	euxassay_003112_12	euxassay_003112_13	euxassay_003112_14	euxassay_003112_15

euxassay_003112_16	euxassay_003112_17	euxassay_003112_18	euxassay_003112_19	euxassay_003112_20

euxassay_003112_21	euxassay_003112_22	euxassay_003112_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 15

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 09 10 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 05 06 07 16 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 12

pons mantle layer

moderate

regional

moderate expression: see section 05 06 07 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 16 17

vagus x ganglion

weak

regional

weak expression: see section 08 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 weak expression: see section 04 05 06 07 08 17 18 19 20 21 22

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 15 16 17 18 19 20

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 21 22 23 moderate expression: see section 07 08 09 10 11 16 17 18 19 20

telencephalon marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4249
Entity Detected:	Kcnip1, Kv channel-interacting protein 1 ( MGI:1917607)
Sequence:	sense strand is shown >T4249 AACATCAGGCAGGCTTTGGGGCTCGGGGCTCGGGCCTCGGAGAAGCCAGTGGCCCGGCTGGGTGCCCGCA CCGGGGGGCGCCTGTGAAGGCTCCCGCGAGCCTCTGGCCCTGGGAGTCAGTGCATGTGCCTGGCTGAAGA AGGCAGCAGCCACGAGCTCCAGGCGCCCCGGCCCCACGTTTTCTGAATACCAAGCTGCAGGCGAGCTGCT CGGGGCTTTTTTGCTTTCTCGCTTTTCCTCTCCTCCAATTCAAAGTGGGCAATCCACACCGATTTCTTTT CAGGGGAGGGAAGAGACAGGGCCTGGGGTCCCAAGACGCACACAAGTCTTCGCTGCCATGGGGGCCGTCA TGGGCACTTTCTCCTCCCTGCAGACCAAACAAAGGCGACCCTCTAAAGACAAGATTGAGGATGAGCTAGA GATGACCATGGTTTGCCACCGGCCTGAGGGACTGGAGCAGCTTGAGGCACAGACGAACTTCACCAAGAGA GAACTGCAAGTCTTGTACCGGGGATTCAAAAACGAGTGCCCTAGCGGTGTGGTCAATGAAGAAACATTCA AGCAGATCTACGCTCAGTTTTTCCCTCACGGAGATGCCAGCACATATGCACATTACCTCTTCAATGCCTT CGACACCACCCAGACAGGCTCTGTAAAGTTCGAGGACTTTGTGACTGCTCTGTCGATTTTACTGAGAGGG ACAGTCCATGAAAAACTAAGGTGGACGTTTAATTTGTATGACATCAATAAAGACGGCTACATAAACAAAG AGGAGATGATGGACATAGTCAAAGCCATCTATGACATGATGGGGAAATACACCTATCCTGTGCTCAAAGA GGACACTCCCAGGCAGCATGTGGATGTCTTCTTCCAGAAAATGGATAAAAATAAAGATGGCATTGTAACG TTAGATGAATTTCTTGAATCATGTCAGGAGGATGACAACATCATGAGATCTCTACAGCTGTTCCAAAATG TCATGTAACTGAGGACACTGGCCATTCTGCTCTCAGAGACACTGACAAACACCTTAATGCCCTGATCTGC CCTTGTTCCAATTTTACACACCAACTCTTGGGACAGAAATACCTTTTACACTTTGGAAGAATTCTCTGCT GAAGACTTTCTACAAAACCTGGCACCACGTGGCTCTGTCTCTGAGGGACGAGCGGAGATCCGACTTTGTT TTGGAAGCATGCCCATCTCTTCATGCTGCTGCCCTGTGGAAGGCCCCTCTGCTTGAGCTTAATCAATAGT GCACAGTTTTATGCTTACACATATCCCCAACTCACTGCCTCCAAGTCAGGCAGACTCTGATGAATCTGAG CCAAATGTGCACCATCCTCCGATGGCCTCCCAAGCCAATGTGCCTGCTTCTCTTCCTCTGGTGGGAAGAA AGAGTGTTCTACGGAACAATTAGAGCTTACCATGAAAATATTGGGAGAGGCAGCACCTAACACATGTAGA ATAGGACTGAATTATTAAGCATGGTGATATCAGATGATGCAAATTGCCCATGTCATTTTTTTCAAAGGTA GGGACAAATGATTCTCCCACACTAGCACCTGTGGTCATAGAGCAAGTCTCTTAACATGCCCAGAAGGGGA ACCACTGTCCAGTGGTCTATCCCTCCTCTCCATCCCCTGCTCAAACCCAGCACTGCATGTCCCTCCAAGA AGGTCCAGAATGCCTGCGAAACGCTGTACTTTTATACCCTGTTCTAATCAATAAACAGAACTATTTCGTA CACTCTCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503251 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003112 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source