EMAGE mouse gene expression spatial database

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EMAGE:31423

Acat2 acetyl-Coenzyme A acetyltransferase 2 ( MGI:87871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003126_01	euxassay_003126_02	euxassay_003126_03	euxassay_003126_04	euxassay_003126_05

euxassay_003126_06	euxassay_003126_07	euxassay_003126_08	euxassay_003126_09	euxassay_003126_10

euxassay_003126_11	euxassay_003126_12	euxassay_003126_13	euxassay_003126_14	euxassay_003126_15

euxassay_003126_16	euxassay_003126_17	euxassay_003126_18	euxassay_003126_19	euxassay_003126_20

euxassay_003126_21	euxassay_003126_22	euxassay_003126_23	euxassay_003126_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 16 20 moderate expression: see section 08 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22

not examined

regional

not examined expression: see section 01 02 03 04 24

neural retina

moderate

regional

moderate expression: see section 02 03 04 24

vagus x ganglion

strong

regional

strong expression: see section 09 18

trigeminal v ganglion

strong

regional

strong expression: see section 09 10 18 19 moderate expression: see section 04 05 06 07 08 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 21 22 23 24

cervical ganglion

strong

regional

strong expression: see section 09 10 moderate expression: see section 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 moderate expression: see section 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 19 moderate expression: see section 07 08

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4212
Entity Detected:	Acat2, acetyl-Coenzyme A acetyltransferase 2 ( MGI:87871)
Sequence:	sense strand is shown >T4212 GGACAAGGCTGAGAGCTAGCAGAGACCAGAGCGACAAGATGAATGCGGGCTCAGACCCCGTGGTCATCGT CTCAGCGGCGCGCACCGCTATAGGCTCCTTCAATGGTGCCCTGTCCACCGTGCCTGTCCACGAAATGGGG ACAACTGTCATCAAAGAAGTCCTGCAGAGGGCCAAGGTGGCTCCAGAAGAGGTGTCCGAGGTCATATTTG GACATGTCTTGACGGCAGGTTGTGGGCAGAATCCTACTCGACAAGCCAGTGTGGGTGCAGGGATCCCCTA TTCGGTTCCAGCATGGAGCTGCCAGATGATCTGTGGCTCGGGCCTAAAAGCCGTGTGTCTTGCAGCCCAG TCCCTAGCCATGGGCGACTCCACCATTGTGGTAGCTGGAGGCATGGAGAATATGAGCAAGGCTCCTCACT TGACTCACCTGAGAACAGGAGTCAGGATGGGTGAGGTGCCACTGGCTGACAGCATACTCTGTGATGGTCT GACAGATGCCTTTCACAACTACCACATGGGCATCACAGCTGAAAACGTAGCCAAAAAATGGCAAGTGAGC AGAGAGGCCCAGGACAAGGTTGCAGTTCTGTCACAGAACAGGGCAGAGCATGCTCAGAAAGCTGGCCACT TTGACAAGGAGATTGTGCCAGTGCTGGTGTCTTCCAGAAAAGGTCTTACTGAAGTGAAAATCGATGAATT TCCCCGTCATGGGAGTAACCTTGAAGCCATGGGCAAGCTGAAACCTTACTTTCTTACTGATGGGACAGGA ACTGTCACCCCAGCGAACGCATCAGGAATGAACGATGGCGCTGCTGCTGTGGTCCTTATGAAGAAGACAG AAGCTGAGAGGCGGATGCTGAAACCTTTAGCAAGAATAGTCTCCTGGTCCCAAGCCGGTGTGGAGCCTTC TGTTATGGGAGTAGGACCAATTCCAGCCATAAAGCAAGCTGTTGCAAAGGCAGGCTGGTCCCTGGAGGAT GTTGACCTGTTTGAAATCAATGAAGCCTTTGCAGCGGTGTCTGCGGCAATAGCTAAAGAACTTGGATTAA ACCCCGAGAAGGTCAACATCGATGGAGGAGCCATTGCCTTGGGCCATCCTCTGGGAGCATCTGGCTGCAG GATTCTAGTGACCTTGCTGCACACCCTGGAGAGAGTGGGCGGGACCCGTGGTGTTGCAGCCCTGTGCATT GGGGGTGGGATGGGAGTCGCAATGTGTGTTCAGAGAGGGTGAACTGCATGACAGCCACCACCTTGGACAG TTCTTGTTAAATCAGTGCAACACTAAGCAGAAAGGGAAACCAGAGGACCAAACTGAGGACAGGAACCCAG GTGGACAGCTTGCTGTACTTTAATGTGAGACACCCAAGGCTACGGCATTGCACCTGACACTGTTATAAAT AAGAGGGAAATCCAATCAGTCATCAAGGGCTCCAGAGTGAACAGCATTTTCATAACTTCCATGTTTATCG TCTTTACTTTCTGGGTGTAATTTTCTCTGATCATTGTTTTTTGTTTTTTTTGTTTTTCTTTGTTTTTGAG TTAGGGTCTCACCATATAGCCTCAAAATCGTTTTGTAGATCAGGTTGACTTTGAACTCCCGGAGATCCAC CTTTCTGTGCCTCCTGAATACTTGAATTAAAGGCATGCACCATTATGCAGGGCCCCCAAAATGATCCATT CAAAACATGGGGATTCGGCTTCTGTTACGAACTTCAGGCTCTTCACTTTAACTATGGTTGGTTCAAACGC CCCTGCGTGCCTGTCTCTCGGTTTCCCATTGATCAAATCAAGACCAATCCTGTAGTGAAAGTCGGATTCA GTGACCTCTTCCAGGCTGAGGTGGGGTAGGTATTCCAGCTGTTCTAGTCAGAAGACTGGCAGTGCCAACA AGTGTGCCTCTGGATTGTTGAAAGGTGGGCAGCAAGCAGCATCCACGTACTTATTTCTCTGCTTGACGGG ATGGGATGTTACCTGCTTCAGATATCTACCTTGACTTCCCCAAAATGATGACTGGAACTGGAACTGACAG TCAAATAAACTCTTTCCTCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4500929 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4500929 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003126 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source