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EMAGE:31428

Commd9 ( MGI:1923751)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_012061_01 euxassay_012061_02 euxassay_012061_03 euxassay_012061_04 euxassay_012061_05
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euxassay_012061_06 euxassay_012061_07 euxassay_012061_08 euxassay_012061_09 euxassay_012061_10
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euxassay_012061_11 euxassay_012061_12 euxassay_012061_13 euxassay_012061_14 euxassay_012061_15
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euxassay_012061_16 euxassay_012061_17 euxassay_012061_18 euxassay_012061_19 euxassay_012061_20
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euxassay_012061_21 euxassay_012061_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
temporal bone
moderate moderate
regionalmoderate expression: see section 01 02 03 weak expression: see section 04 17 18 19 20 21
humerus
strong strong
regionalstrong expression: see section 01 02 03 weak expression: see section 04 19 20 21 22
fibula
strong strong
regionalstrong expression: see section 01 weak expression: see section 03
pelvic girdle skeleton
weak weak
regionalweak expression: see section 08 15 16
rib
strong strong
regionalstrong expression: see section 01 moderate expression: see section 02 05 06 weak expression: see section 03 04 07 16
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 weak expression: see section 08 13
viscerocranium
weak weak
regionalweak expression: see section 08 18 19
otic capsule
weak weak
regionalweak expression: see section 08 15 16
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 04 05 06 07 weak expression: see section 03 08 12 13 14 15 16 17 18 19
basioccipital bone
weak weak
regionalweak expression: see section 08 09 10 11 12 13
basisphenoid bone
weak weak
regionalweak expression: see section 13
tibia
strong strong
regionalstrong expression: see section 01 moderate expression: see section 02 weak expression: see section 03
scapula
strong strong
regionalstrong expression: see section 01 02 03 weak expression: see section 18 19
femur
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 04 05 weak expression: see section 06 17 18 19 20 21
exoccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 03 04 weak expression: see section 17 18 19 20 21 22
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 weak expression: see section 07 08 09 18 19 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36182
Entity Detected:Commd9, ( MGI:1923751)
Sequence:sense strand is shown

>T36182
CCTTGACTCAGAAAGCCTCCTAGATAAGACGTGCTCCAGCTTGTCTGTGCCCCAGGGGGAGGCAGCGCAA
CTGCTGCAGGCTCTGCACCACTTCACCAGGCTGGTGGCCTTCCGTGACCTGTCCTCTGCTGAGGCGATTC
TGGCTCTCTTTCCGGAAAATTTCCACCAAAACCTCAAAAACCTGCTGACAAAAATCATCGTGGAGCACAT
ATCGACTTGGAGAGCTGAAGCCCAAGCAAACCAGATCTCTCTGCCACGCCTGGTGGACCTGGACTGGAGA
GTGGACATCAAAACCTCCTCTGACAACATCAGCCGCATGGCTGTCCCCACCTGCCTGCTCCAGATGAAGA
TCCAGGAGGATCCTAGTCTGTGTGGAGAGAAACCCTCCATCTCGGCTGTGACCATGGAGCTGAGCAAAGA
GACGCTGGACACCATGTTAGATGGGCTGGGTCGCATTCGGGACCAGCTTTCTGCTGTAGCTAATAAATAA
GGCAACCAGCTATTGGCCTGCGAAGGTGTGGTTGTCCGCACAGAGGCTGACGGTGGGCAGACAGTGTGAT
GGAGGGGCTGCTGCTACTCAGAAGGCTCAGTAGAGGAACGGAAGCCCACGCCCATTGTTCCTCTCTTTTC
TTCCTCCTGTTTTTCCTGAAGATGAGAAGAAGCCTCCATCTCTGAGAAGGCCCTGTGTGGTGATAAGCTA
CCGCCAGCCGTGGCAGCCCGAGGGAATGTGTTCTTTATCAGCCGCCTGTGCGTGCTGCTGTGGAGAGCTC
AGGCCGGCACCGTGGCTCTCACCTAGTGAGGTTACCTCCAGCATTAGCTCGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66050. Forward Primer - name:066050_F_cDNA_Commd9, sequence:CCTTGACTCAGAAAGCCTCCTA; Reverse Primer - name:066050_N_SP6_cDNA_Commd9, sequence:CCGAGCTAATGCTGGAGGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012061 same experiment
 EMAGE:31429 same embryo
 EMAGE:31427 same embryo
 EMAGE:31431 same embryo
 EMAGE:31430 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS