EMAGE mouse gene expression spatial database

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EMAGE:31433

Vps33a vacuolar protein sorting 33A (yeast) ( MGI:1924823)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003122_01	euxassay_003122_02	euxassay_003122_03	euxassay_003122_04	euxassay_003122_05

euxassay_003122_06	euxassay_003122_07	euxassay_003122_08	euxassay_003122_09	euxassay_003122_10

euxassay_003122_11	euxassay_003122_12	euxassay_003122_13	euxassay_003122_14	euxassay_003122_15

euxassay_003122_16	euxassay_003122_17	euxassay_003122_18	euxassay_003122_19	euxassay_003122_20

euxassay_003122_21	euxassay_003122_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 16 17 moderate expression: see section 15

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

cervical ganglion

moderate

regional

moderate expression: see section 08 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4214
Entity Detected:	Vps33a, vacuolar protein sorting 33A (yeast) ( MGI:1924823)
Sequence:	sense strand is shown >T4214 GGGGAAATGCGGGTGCCCGGGCAAGATGGCGGCGCACCTGTCGTACGGGCGAGTGAACCTGAACGTGCTG CGCGAGGCGGTGCGTCGGGAGCTGCGCGAGTTCCTGGACAAGTGCGCTGGGAGCAAGGCAATAGTGTGGG ATGAGTACTTAACAGGACCATTTGGACTGATCGCACAGTACTCACTACTAAAGGAACATGAAGTGGAAAA AATGTTCACACTTAAAGGAAGTCGTCTGCCCGCAGCTGATGTCAAGAACATCATTTTTTTGGTCAGACCC AGGCTAGAGCTGATGGACATAATTGCTGAGAATGTACTCAGTGAGGACAGGCGTGGCCCTACCAGGGATT TCCATATTTTGTTTGTGCCTCGCCGGAGCCTACTGTGTGAGCAGCGGCTGAAGGACCTGGGGGTTTTGGG ATCCTTTATTCATCGAGAAGAATACAGCTTGGATCTCATTCCATTTGATGGGGACCTCTTATCCATGGAG TCAGAGGGCGCTTTCAAAGAGTGCTACCTGGAAGGCGACCAGACCAGCCTGTACCACGCAGCCAAGGGGC TGATGACCCTGCAGGCGCTGTATGGAACTATCCCCCAGATCTTTGGGAAAGGAGAGTGTGCTCGGCAAGT GGCCAATATGATGGTCAGGATGAAGAGGGAGTTCACAGGAAGCCAGAACTCAGTGTTCCCCGTGTTTGAT AACCTCTTGCTGCTTGATCGCAATGTGGATCTCCTGACGCCACTCGCCTCCCAGCTCACGTATGAAGGCC TCATCGATGAGATTTACGGCATTCAGAACAGTTATGTGAAGTTGCCTCCAGAGAAGTTTGCACCCAAGAA GCAGGGTGGCGGCAGTGGTGGGAAGGACCTTCCCACGGAGGCCAAGAAGCTTCAGCTCAACTCCGCAGAG GAGCTCTACGCCGAGATCCGAGACAAGAACTTCAACGCTGTGGGCTCTGTGCTCAGCAAGAAGGCGAAGA TCATCTCTGCAGCGTTCGAGGAGCGACACAACGCTAAGACAGTCGGGGAGATCAAGCAGTTTGCGTCCCA GCTACCCCACATGCAGGCAGCCAGGGGATCGCTCGCCAACCACACCTCAATTGCAGAGTTAATCAAAGAC GTCACCACTTCTGAAGACTTCTTTGATAAGTTGACAGTGGAGCAGGAGTTCATGTCCGGGATAGACACTG ATAAGGTCAACAGTTACATCGAGGACTGCATTGCCCAAAAGCATCCACTCATCAAGGTGCTGAGACTGGT TTGCCTCCAGTCTGTGTGCAACAGCGGGCTCAAGCAGAAGGTGCTGGACTATTACAGAAGGGAGATCCTC CAGACCTACGGCTATGAGCACATACTGACCTTGAACAACCTGGAGAAGGCCGGCCTGCTCAAGGCTCAGA CAGGGGGCAGAAACAATTACCCAACAATCCGGAAAACACTGCGCCTCTGGATGGACGATGTCAATGAACA AAACCCCACAGACATATCCTATGTGTACAGCGGTTATGCTCCACTCAGCGTGCGACTGGCTCAGCTCCTT TCCCGGCCAGGCTGGCGGAGCATCGAGGAGGTTCTCCGCATTCTCCCAGGGCCCCACTTTGAAGAAAGAC AGCCACTGCCCACCGGGCTGCAGAAGAAACGCCAGCCAGGAGAAAACAGAGTCACACTGGTATTTTTCCT CGGGGGCGTCACCTTTGCGGAAATAGCCGCCCTGCGATTTTTGTCCCAGCTGGAAGATGGAGGCACCGAG TATGTCATTGCGACCACTAAACTGATGAATGGAAACAGCTGGATAGAAGCTCTCATGGAAAAGCCTTTCT AGAGTATCGGAGGAGACGTGACAGGTCACCACACAATGAGCTGCCTCTGTGAACGTGTTGCCAAAGGATG TAAGGCCAGGAACAGGAGGACGGAAGCAGTGGGCGCATGTTCCTTCCTGCTCCTCTCTATGCTCTTAATC CTAAAGAAGGGACAAGCAACCTGGATGAAGGGATGATGAACTGGGCATAAGAGACAGAGGCAGGAGGATT GCTGCAAGCTTGAGGACAACCTGGGCTCCATAATGAGTTTCAGGCCAAAGCTCTCCCTTCCAGAAATAAA GGAGATCCTGTTTTGGAATTTTTAAGTCTCAAGTCTTCATTTTCTGGAAATGGCTAAGACTATGGGTCTG ATAGCCCTGGCAACCACATGACTGCACAGACATTAAAAGCAGAGCTGAGGGGACGAATTCAAAAAGAGAA TCAGAATTCTGACCACTGATCCACAGTGGCATATTCTAGAAGATGTCCCATGCCCAGGACTGTGGTTTTC TGTTGAATTGGAAGCCAGCATTGTCCAGTGTGTGCACTTTCCTGCAAAGCACATTTGTGTCTCCAGTCTC TACCGAGTTATTCTTAAGGCGAGCTTTGTTTCCTTCTGAACAACTAAGAGGTTCGAAGGGGCTTCTTGGC ATAATGACATTTCTGCTCTCTGCGTTAGCCATGACTGTGCAGGTCTCTGCCCAGAAGGGACTGATCAAGA GTGAGCTGAGCGCCAGGCAGCCTCTTCCAGCTAACCTTCCAAAGGCACTTCTGAGCGTGCATGTTGGTAA CTCCTGGACTCACCAGGGTAGGCGCTCCTGCCGGGACTTCTGTTTTCCTCCATGTCCTCCATGGGCAGTT CTTCTGTTCTTTCAATATCATGAAGCTCTGTCTCCGTGGTAACTGCCACACACAGCTCTCTAGCCCTCTA GCTGTACTCAACTCTGCACACCCCCTTTCCCTGAAAATACTCAGGTGGTCAGTGCACTTCGGTCAGTGTC AGTCACAAGAGACAAGCAGCACCAGTGCTCAGGGAGTGGAGCACGGCCGGAAGCAGAGCAGGCATGGCCT CCTGATCATCTCAGCATCTGAAGGAGCTAAGAGCACCAAGTGGGGGACCACGTTGATGGCTTTCCTGTCA TCACTCAAGACTCCAGAAAAGCTAGCCTGTCTCAGCCGGTCCTGGTCCTTGCCCAGGTGCAGTTTCTATG GTTAATATTTTCTTCTTGTTTTTCTCATGTAGAATCAGGAGCAATACACTGCACATTATAAGTAACCAGA GTTGGGTATTTAAAAGAAAAAAACCTAGGAATGTAGGATGGCAAAGATGATATTTGCTTCTCGTGGCGTT AATTCAGTATTGGGAGAGTCAGGCGGTGTGGGGGGCATTCCCAAATGGGAAATCATTCTTAACTTAGAAG ACTCTTTTTCAGATTCTTGTTACTCCAGGGAGACTAGTTTTACCTTTGAGCCCCAGCAATGGGTAGTTTC AGCTGCCAGGGGCTGCAATCCACCTTGTCCTCAGTGTTTTCCACTCTAGGGATTCAGGACAAGTCTGTCC ACGTCTCCTTGGCGTGGGTGTTTGTCCAGTGAACAGACACTCAGTTCTAATCTCATGTGTCTGCATTTGT TTCTGAGTCTATATTTGCTTTCTCAGAGCATAGCAAGGTCAACTCCTAAAAATGTACCCAGCAGAAGACC TTTTTGAAAAACCACACACTTGCTTCCTCTGTATTCACACACAAAGTGGTGGTATCATACATTATGCCCT GTGATGTAAGTCCCCTTAGTGCTCAAAGCTGCCTTTGTCTCTTAAGAGCGTGCACTGAGAAAGGCTGTGG GAACGGAAGGCTTATTCTCAGACAGGGTGGTTTGTTTACCCCATGTCTGGACTTGTCCTGAGTCTGAGGA CCACTCTGCTGCACTGCCCGTCCTGATTAAAGACGTCTCTGTAGATGCTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:4501207 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501207 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003122 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source