EMAGE mouse gene expression spatial database

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EMAGE:31442

Zfp451 zinc finger protein 451 ( MGI:2137896)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000068_01	euxassay_000068_02	euxassay_000068_03	euxassay_000068_04	euxassay_000068_05

euxassay_000068_06	euxassay_000068_07	euxassay_000068_08	euxassay_000068_09	euxassay_000068_10

euxassay_000068_11	euxassay_000068_12	euxassay_000068_13	euxassay_000068_14	euxassay_000068_15

euxassay_000068_16	euxassay_000068_17	euxassay_000068_18	euxassay_000068_19	euxassay_000068_20

euxassay_000068_21	euxassay_000068_22	euxassay_000068_23	euxassay_000068_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex ventricular layer

moderate

homogeneous

moderate expression: see section 24 weak expression: see section 03 04 05 06 07 08 21 22 23

physiological umbilical hernia

moderate

regional

moderate expression: see section 05 weak expression: see section 06 07

telencephalon ventricular layer

moderate

regional

moderate expression: see section 10 13 15 16 20 weak expression: see section 09 11 12 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 14 15 16 weak expression: see section 11 12 17 18 19

metanephros

weak

homogeneous

weak expression: see section 08 09 10 11 18 19 20 21 22 23

testis

strong

spotted

strong expression: see section 07 08 moderate expression: see section 06 23 24

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1570
Entity Detected:	Zfp451, zinc finger protein 451 ( MGI:2137896)
Sequence:	sense strand is shown >T1570 AGAAAAGTGAGGAGGAGCAGCAGTATGTGATCAAGTGTGGCATCTGCACCAAGGCATTCCAGAACACGGA GAGCGCTCAGCAGCACTTCCACAGGAAGCACGCGGCCCTCCAGAAACCCACCGCGACCCCAGGGGGAGCC AACAGAAGCAGCACATGCCAGCTGGCTGCTAGTGCCTCACATGCTGAGAAAAACCTGAAACAGCCTAGCT CTCAGAAACATTCAGACGTGGAAAAAGGAGCTGAGCATGATGTACGCTGCCAGAACATAGAGGAGGAAGT TGAGCTTCCGGATGTGGACTACCTGCGGACCATGACTCACATAGTCTTTGTAGATTTTGATAACTGGTCC AACTTTTTTGGTCATCTACCTGGGCATCTTAATCAAGGAACGTTTATTTGGGGCTTTCAAGGGGGAAACA CCAACTGGAAGCCCCCGCTCAGCTGTAAGGTCTATAATTACTTGAGCAGGATTGGCTGCTTCTTCCTTCA TCCTCGCTGCAGTAAAAGAAAAGATGCCGCGGATTTTGCCATATGTA
Notes:	The probe template was PCR amplified from IMAGE:933209 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:933209 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000068 same experiment
	EMAGE:30393 same embryo
	EMAGE:32028 same embryo
	EMAGE:30632 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source