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EMAGE:31445

BC064033 ( MGI:3039626)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445
euxassay_006844_01 euxassay_006844_02 euxassay_006844_03 euxassay_006844_04 euxassay_006844_05
EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445
euxassay_006844_06 euxassay_006844_07 euxassay_006844_08 euxassay_006844_09 euxassay_006844_10
EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445
euxassay_006844_11 euxassay_006844_12 euxassay_006844_13 euxassay_006844_14 euxassay_006844_15
EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445 EMAGE:31445
euxassay_006844_16 euxassay_006844_17 euxassay_006844_18 euxassay_006844_19 euxassay_006844_20
EMAGE:31445 EMAGE:31445 EMAGE:31445
euxassay_006844_21 euxassay_006844_22 euxassay_006844_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 10 11 17
clavicle
strong strong
regionalstrong expression: see section 09 10 16 17 moderate expression: see section 18
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 09
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 14
viscerocranium
moderate moderate
regionalmoderate expression: see section 07 19 weak expression: see section 08 09
otic capsule
moderate moderate
regionalmoderate expression: see section 08 09 10 16 17 18 19 weak expression: see section 04 05 06 07 20 21 22
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 18
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 19 20
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 18 19 22 23 weak expression: see section 03 04 05 20 21
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22 23 weak expression: see section 02
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18
cervical ganglion
moderate moderate
regionalmoderate expression: see section 10 17
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 07 19 20 22 23 weak expression: see section 01 02 03 04 05 06 08 21
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 23
not examined not examined
regionalnot examined expression: see section 08
mandible
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 12 16 17 18 19 20 21 22 23
liver right lobe
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 12 14 15
rib
strong strong
regionalstrong expression: see section 05 06 07 moderate expression: see section 01 02 03 04 08 09 19 20 21 22 23 weak expression: see section 10 11 16 17 18
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 17 18 weak expression: see section 12 14 16
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 19
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 14 15 weak expression: see section 16
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 11 13 17 18 19 weak expression: see section 12 20
not examined not examined
regionalnot examined expression: see section 09
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 16 18 19
respiratory system
moderate moderate
regionalmoderate expression: see section 07
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 16 17 18 weak expression: see section 11 12 13 14 15
maxilla
moderate moderate
regionalmoderate expression: see section 05 06 07 08 10 18 19 20 21
nucleus pulposus
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 14 15 weak expression: see section 12 16
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13 14
liver left lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 12 15 16
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13 14
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8187
Entity Detected:BC064033, ( MGI:3039626)
Sequence:sense strand is shown

>T8187
GAGAGGTACCAACTTCTGTCCCACCCAAGAGGCTGCATCCGCCTCCATCCTGTGGAGCCAGGGAGAGGCC
CTTGCTTTCCTTATAGACAAGAAAGGGCAGTAAGAACTCTGTCCTCTCGCTGAGAAGAGCAGGGGTCCAC
CTGCAGCCCCTGGGGTCCCGCAGGAATAGAAGGTCAGCTTGTCTCCCTCCTGGAAGATGTCCTGGAAGGC
GCTGACGATTCTGCTGGTATTCTCCAGCACCCAGGCCACTGCGTCCTGCAGGTGGAGCAGGGCCGCACTG
TTCCCAGCTGCCCATCGGCCAAAGAGGTCCTTGTCACTGCCATTGAATCCAGTCCTGCAGACCTCCCTGG
AGGAGGTGGAACTGCTGTATGAGCTCTTGCTAGCTGAAATTGAGATCAGCCCAGACCTGGAGATCTCCAT
CAAGGACGAGGAGCTAGCTTCCCTGCGGAAGGCCTTGAGTTTCCACTCAATCTGCAATAACATAATCCCC
AAGCGTATCCCAGATATCCGAAGGCTGAGTGCCAACCTGGCAAACCACCCTGGAATCCTCAAGAAAGAAG
ACTTTGAGAGGATAACATTAACCCTGGCGTACACAGCCTATCGGACAGCCTTATCTGAAGGGCATCAGAA
GGACATCTGGGCTCAGTCCCTCATCAGCCTATTCCAGGCCCTGAGGCATGACTTGATGCGGTCCTCGAGC
CCTGCTGTGTCATCCTGAGAGAATGGCTCATGCTAGAACTTTGAAGCAGGAACAGGCACACACAGTCTTC
TAGAACTTTCATCCTCTACTGCACTTTCAGAGAAAAGTATATACTTCCCACACAGAATAGCAAAGATAAA
TGAGTCACCCCAATATTTTTTGTCCCTTGTTGCTTCCAGACAGACATATCCGACCTATGTTATAATGTTA
CCTGAGAAAAGGCTAGACTGGACTTTCAAGATGCCTCCAGAGGCCAACTGGTCTACCTGGTAATGAGCAG
ACTTCTGAGATATACTTACACACATACCCAAGAGTAGGGACTGAGGATGGAGTCTGAGCATGGCAGGAGG
ATGGTGGGCAGATTCCTTTGGTTCTAAGGGATCTGTGTTGAATGAATATTTTCTGGCAGGTTCTATGGTA
AATATAAAAAAGGCAGAGATGCATTCAAATTAATATGCTATTAGCCAAGAAGGATATACTTGGCTTGCCC
CAAAGCCATGAAGAAGACTCTGTATTTTGGTGACCTACTTGACTTGGTGGAAATGCTAGCAGTCCACCCA
TGCCCTATCATTTCAATGTAGAAGCCAGGCTAAAGCATAGTGCCTTCCTAATGAAAGAGGTAACACCACT
ATGCGTGTTTTTCCTAAAATACCATAGCACTGTCAGCGACTTGGGTGCTCCTAAAAAAATTCGCTTTCAG
ATGACAGATTGTTTACCTTTCAAATGCTGATTTTTTTTCTTTCAAAATGTTAGTTTGATATCTGTTCATT
ATTTATATTAAATACTGGTTGATATTTAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:851727 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:851727 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006844 same experiment
 EMAGE:29384 same embryo
 EMAGE:31394 same embryo
 EMAGE:31422 same embryo
 EMAGE:31453 same embryo
 EMAGE:31434 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS