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EMAGE:31452

Acsbg1 acyl-CoA synthetase bubblegum family member 1 ( MGI:2385656)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_012058_01 euxassay_012058_02 euxassay_012058_03 euxassay_012058_04 euxassay_012058_05
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euxassay_012058_11 euxassay_012058_12 euxassay_012058_13 euxassay_012058_14 euxassay_012058_15
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euxassay_012058_16 euxassay_012058_17 euxassay_012058_18 euxassay_012058_19 euxassay_012058_20
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euxassay_012058_21 euxassay_012058_22 euxassay_012058_23 euxassay_012058_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
temporal bone
moderate moderate
regionalmoderate expression: see section 01 weak expression: see section 03 04 05 18 21 22
vault of skull
moderate moderate
spottedmoderate expression: see section 01 02 weak expression: see section 03
humerus
moderate moderate
regionalmoderate expression: see section 01 weak expression: see section 02 03 20 21 22 23 24
fibula
moderate moderate
regionalmoderate expression: see section 02 weak expression: see section 04
pelvic girdle skeleton
weak weak
regionalweak expression: see section 09 16
rib
weak weak
regionalweak expression: see section 03 04 05 06 15 16 17 18 19 20 21
axial skeleton
weak weak
regionalweak expression: see section 04 05 06 07 08 09 12 13 14
testis
strong strong
regionalstrong expression: see section 05 06 07 17 18
viscerocranium
weak weak
regionalweak expression: see section 18 19 20
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 03 04 05 07 08 09 10 11 12 15 16 17 18 19 20 21
basioccipital bone
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14
basisphenoid bone
weak weak
regionalweak expression: see section 10 11 13 14
tibia
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 03
scapula
moderate moderate
regionalmoderate expression: see section 01 weak expression: see section 02 03 19 20
femur
weak weak
regionalweak expression: see section 03 04 05 06 18 19 20 21 22
exoccipital bone
weak weak
regionalweak expression: see section 04 17 18
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 weak expression: see section 03 04 06 07 08 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30693
Entity Detected:Acsbg1, acyl-CoA synthetase bubblegum family member 1 ( MGI:2385656)
Sequence:sense strand is shown

>T30693
CCAACCAGTGCTGTGTGCTCGTCTACACATCAGGCACCACGGGAAACCCCAAGGGTGTGATGCTGAGTCA
AGACAATATCACATGGACAGCACGGTATGGCAGTCAGGCTGGGGACATCCAGCCAGCAGAAGTGCAGCAG
GAGGTAGTGGTCAGCTACTTGCCTCTCAGCCACATCGCTGCCCAGATCTACGACCTGTGGACAGGCATCC
AGTGGGGAGCCCAGGTCTGCTTTGCAGATCCGGACGCCCTGAAGGGGACCCTGGTAAACACACTGCGGGA
GGTGGAGCCCACATCCCACATGGGAGTGCCGCGTGTGTGGGAGAAGATCATGGAAAGGATCCAGGAGGTG
GCCGCTCAGTCTGGCTTCATCCGCCGCAAGATGCTGCTGTGGGCCATGTCAGTCACCTTGGAACAGAACC
TTACCTGCCCTAGCAATGACCTGAAGCCCTTCACCAGCAGACTGGCGGATTACCTAGTGTTAGCCAGGGT
CCGTCAGGCTCTGGGCTTTGCCAAGTGTCAGAAAAACTTCTACGGAGCAGCTCCGATGACGGCAGAGACG
CAGCGCTTCTTCCTGGGCCTCAACATCCGCCTGTATGCAGGCTATGGCCTCAGCGAGAGCACAGGCCCCC
ACTTCATGTCCAGCCCCTACAACTATCGGCTTTACAGCTCCGGCAGGGTGGTACCCGGCTGCCGGGTGAA
GCTGGTGAATCAGGATGCAGATGGCATCGGTGAGATCTGCCTGTGGGGCCGCACCATCTTCATGGGTTAT
CTGAACATGGAGGACAAAACCTGTGAGGCCATTGATTCGGAAGGCTGGCTGCACACAGGTGACATGGGCC
GCCTGGATGCTGACGGCTTCCTCTACATCACCGGGCGCCTCAAAGAATTAATCATCACTGCGGGCGGCGA
GAACGTGCCCCCAGTGCCTATCGAGGAGGCCGTGAAGATGGAGCTGCCCATCATCAGCAGTGCCATGCTG
ATCGGGGACCAGAGGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6832261), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59625. Forward Primer - name:059625_F_IRAV118_d06_Lpd, sequence:CCAACCAGTGCTGTGTGC; Reverse Primer - name:059625_R_SP6_IRAV118_d06_Lpd, sequence:CTTCCTCTGGTCCCCGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012058 same experiment
 EMAGE:31432 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS