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EMAGE:31453

Arl11 ( MGI:2444054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31453 EMAGE:31453 EMAGE:31453 EMAGE:31453 EMAGE:31453
euxassay_006841_01 euxassay_006841_02 euxassay_006841_03 euxassay_006841_04 euxassay_006841_05
EMAGE:31453 EMAGE:31453 EMAGE:31453 EMAGE:31453 EMAGE:31453
euxassay_006841_06 euxassay_006841_07 euxassay_006841_08 euxassay_006841_09 euxassay_006841_10
EMAGE:31453 EMAGE:31453 EMAGE:31453 EMAGE:31453 EMAGE:31453
euxassay_006841_11 euxassay_006841_12 euxassay_006841_13 euxassay_006841_14 euxassay_006841_15
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euxassay_006841_16 euxassay_006841_17 euxassay_006841_18 euxassay_006841_19 euxassay_006841_20
EMAGE:31453
euxassay_006841_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 13 14 weak expression: see section 09
liver right lobe
moderate moderate
regionalmoderate expression: see section 15 16 17 18 19 20 weak expression: see section 12 13 14 21
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 09 15
clavicle
strong strong
regionalstrong expression: see section 15 moderate expression: see section 08 09 weak expression: see section 14 16
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 06 16 17
dorsal root ganglion
weak weak
regionalweak expression: see section 08 09 11 15 16 17 18
submandibular gland primordium
strong strong
regionalstrong expression: see section 15 16 17 moderate expression: see section 06 07 08 09 14
thymus primordium
weak weak
regionalweak expression: see section 10 11 12 13 14
trigeminal v ganglion
weak weak
regionalweak expression: see section 03 04 05 06 07 15 16 17 18 19 20
vibrissa
weak weak
regionalweak expression: see section 04 05 19 20
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 12 13 14 15 16 17 18 19 20 weak expression: see section 07 08 09 10
cervical ganglion
weak weak
regionalweak expression: see section 09 15 16
liver left lobe
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 09 12 weak expression: see section 13
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8189
Entity Detected:Arl11, ( MGI:2444054)
Sequence:sense strand is shown

>T8189
CCCCAATATGGGAGGATGTCTGCCCTAACCACTCGAGAGAGGAAGCAAAAACAGGACAGATTGCAACAGC
CACTACACTTCCTTCATATCCTGCTCTTGGGACATGGCGACCAGCAGAAAGAAGCTTGATTGTGGAGAGG
TGCCCTAGCGGAGGGATGTCTGCCACAGTCCAGGCAACCACAGAGGGTAGGAGCTCAGTGCATGCATGCA
TGGATTTGCATTTCTTCAGAAAAGAGTAGCTGATGTGCGTCCTCAGCCTTTCTGCAGGAGGATTTACTAG
GGGCCATCATGGGCTCTGTGAATTCCAGAGGTCACAAGGCAGAGGCCCAGGTGGTAATGATGGGCCTCGA
CTCTGCTGGCAAGACCACAATTCTGTACAAACTGAAAGGAAACCAGCTGGTGGACACCCTACCCACTGTT
GGTTTTAATGTAGAGCCTCTTGAGGCTCCTGGACATGTGTCACTGACTCTCTGGGACATTGGGGGACAGA
CCCAGCTCAGAGCTACCTGGAAGGACTACCTGGAAGGCATCGACCTCCTTGTGTACGTGCTGGACAGCAC
AGATGAAGCCCGCTTGCCTGAGGCAGTGGCTGAGCTCAAGGAAGTCCTGGAAGACCCCAACATGGCCGGC
GTCCCCTTCTTGGTACTGGCCAACAAGCAGGAGGCTCCTGGTGCTCTGCCGTTGCTTGAAATCAGAAACA
GGCTGGGCCTGGAAGGGTTCCAAAAGCATTGCTGGGAGCTGCGGGCTTGCAGCGCTCTCACAGGACAGGG
GCTGCAGGAAGCCCTGCAGAGCCTGCTGCATTTGCTGAAGTCCCGCTGAAATGCAGTAACCTCTGACTCC
AGGCTGGAGCAGGACAGGGAGCAGGGTCAGGCGGACCCCGTGAGCGGGCAGAACAGCTTATCCTGGCTAT
GAACAGACGGGGCCAGTTGAACCTTGAGAAAAGGAAGCTCAGTTTACCACACGAGAAACCACAATTCTCT
TGGACAGTATATTTTCTTCTCAGGACTTATTCTCAGTGGCAGTTATGCTGAAAATCAACTGTTCTTCAAG
TAACAATAGTCAGAGGGAATATAAAAATTCTCACAATACCCCGGGTAACAGCCCACTACAAAGTGCCAAT
GTAACAAAGTTGGTGACATAGTAATTGACCAAGTTCACAAATGAAAAGAGGTTCCAAAACTGCATCTGAG
TGTCACCTGGCCTATCACTGTCATCTGAGTGTCACCTGGCCTATCACTGTCATCTGAGTGTCATTGGGCC
TATCACTGTCATCAGGAGCAACTCTTCCTGCTGGGCTCTGGGCAGGAGCCACTGTCACTTTTGTACACTT
AGCTGGGCTGACAGCAGTGAGGGGCAGGTCTGCTCCCAGGATGCTGCAGGAATCCTCCCAGAATTCCCCC
TGCTTTCCAACTCCAACTAACTTTTCTCTGCCCTACATCATATGTTGTAAAAAAGTAAAAATAAAACTCC
ACTTATAATCTCAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:864589 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:864589 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006841 same experiment
 EMAGE:29384 same embryo
 EMAGE:31394 same embryo
 EMAGE:31422 same embryo
 EMAGE:31445 same embryo
 EMAGE:31434 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS