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EMAGE:31458

Mgll ( MGI:1346042)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 09 10 11 12
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 11 12 13 14
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 12 13 14
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
dorsal grey horn
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 11 12 13
esophagus
moderate moderate
regionalmoderate expression: see section 09 10
pancreas
moderate moderate
regionalmoderate expression: see section 06 07 08 09
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 12 13 14
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 02 03 04 05 15 16 17 18 19
cerebral cortex marginal layer
strong strong
homogeneousstrong expression: see section 04 05 06 07 08 09 moderate expression: see section 03
medulla oblongata alar plate ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 11 12 13 14
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 07 15 16 17 18
hindgut
moderate moderate
regionalmoderate expression: see section 09 10 11 12
telencephalon marginal layer
strong strong
homogeneousstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20
liver lobe
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4585
Entity Detected:Mgll, ( MGI:1346042)
Sequence:sense strand is shown

>T4585
CGCGCGCAGTAGTCTGGCTCTAGCCCGCCGCAGACCCGGACCCGCGTGGTGCTCGGGGAACGTGACAGCG
GCGCTCGTGGCCCGGGGCCTAAAGACCCCTGCAGGATGCCTGAGGCAAGTTCACCCAGGCGAACTCCACA
GAATGTTCCCTACCAGGACCTGCCTCACCTGGTCAATGCAGACGGACAGTACCTCTTTTGTAGATACTGG
AAGCCCAGTGGCACACCCAAGGCCCTCATCTTTGTGTCCCATGGAGCTGGGGAACACTGTGGCCGTTATG
ATGAGCTGGCTCATATGTTGAAGGGGCTGGACATGCTGGTATTTGCCCATGACCATGTTGGCCATGGGCA
GAGTGAGGGAGAGAGGATGGTGGTGTCGGACTTCCAAGTTTTTGTCAGAGATGTGCTGCAACACGTGGAC
ACCATCCAGAAGGACTACCCCGACGTCCCCATCTTCCTCCTGGGCCACTCCATGGGCGGTGCCATCTCCA
TCCTAGTGGCTGCAGAGAGGCCAACCTACTTTTCTGGCATGGTCCTGATTTCACCTCTGGTCCTTGCCAA
TCCGGAATCTGCATCGACTTTGAAGGTCCTTGCTGCCAAACTGCTCAATTTTGTCCTGCCAAATATGACC
TTGGGGCGCATTGACTCCAGCGTGCTGTCTCGGAACAAGTCGGAGGTTGACCTGTACAACTCTGACCCAC
TCGTCTGCCGAGCAGGGCTGAAGGTGTGCTTTGGCATACAGCTGCTGAATGCCGTCGCAAGAGTGGAGCG
AGCAATGCCCAGGCTGACACTGCCATTCCTGCTGCTGCAGGGTTCTGCTGACCGGCTTTGCGACAGCAAA
GGTGCCTACCTGCTCATGGAATCATCCCGGAGTCAGGACAAAACACTCAAGATGTATGAAGGTGCCTATC
ACGTCCTCCACAGGGAGCTTCCGGAAGTGACCAACTCCGTCCTCCATGAAGTAAACTCGTGGGTGTCTCA
CAGGATAGCAGCAGCAGGAGCTGGGTGTCCACCCTGAACATGCTGGCCAGGCAACCAGCTCATGGTCTGG
GGGGAGGCAGGCAGAGGAAGGGCAGTGCTGGCTGGCTCAAAAAAAAAAAAAAAAATCACAAATTGGAGGA
ACCCTTAGTACCCTAGTGCAACTTGCTTTGGGAAAAAAAAAAAGGATCATTTGTCATATAGATGGCTACT
GGCCACTGATCTACTACCTTAAGGAGCAGGCACTTTATGAAGGAGGGAGTATATTTTCTACACAGAGACT
GTGTGGAAATATCCTTTGAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4986023 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4986023 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002003 same experiment
 EMAGE:29538 same embryo
 EMAGE:31868 same embryo
 EMAGE:29541 same embryo
 EMAGE:30546 same embryo
 EMAGE:30545 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS