EMAGE mouse gene expression spatial database

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EMAGE:31465

Osr1 odd-skipped related 1 (Drosophila) ( MGI:1344424)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002499_01	euxassay_002499_02	euxassay_002499_03	euxassay_002499_04	euxassay_002499_05

euxassay_002499_06	euxassay_002499_07	euxassay_002499_08	euxassay_002499_09	euxassay_002499_10

euxassay_002499_11	euxassay_002499_12	euxassay_002499_13	euxassay_002499_14	euxassay_002499_15

euxassay_002499_16	euxassay_002499_17	euxassay_002499_18	euxassay_002499_19	euxassay_002499_20

euxassay_002499_21	euxassay_002499_22	euxassay_002499_23	euxassay_002499_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 5 mesenchyme

strong

regional

strong expression: see section 08 09 20 21 22

knee mesenchyme

strong

regional

strong expression: see section 03 04 05 24

midbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 08 09 10 19 21 22

interdigital region between hindlimb digits 1 and 2 mesenchyme

strong

regional

strong expression: see section 06 07 08 09 20 21 22 23

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower leg mesenchyme

strong

regional

strong expression: see section 04 05 24

bladder

moderate

regional

moderate expression: see section 12 13 14 15 16

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 06 09 10 19 21 22

spinal cord meninges

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

upper lip

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

lower lip

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

interdigital region between hindlimb digits 2 and 3 mesenchyme

strong

regional

strong expression: see section 06 07 08 09 20 21 22 23

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 09 19 21 22

interdigital region between hindlimb digits 3 and 4 mesenchyme

strong

regional

strong expression: see section 06 07 08 09 20 21 22 23

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 15 16 18 19 20 21 22 23 24

tongue

weak

regional

weak expression: see section 10 11 12 13 14 15 16

diencephalon meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

foot mesenchyme

strong

regional

strong expression: see section 09 20

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 06 08 09 10 19 20 21 22

interdigital region between hindlimb digits 4 and 5 mesenchyme

strong

regional

strong expression: see section 07 08 09 20 22 23

body-wall mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 24

tail mesenchyme

strong

regional

strong expression: see section 13 14 15 16 17 18

glans of male genital tubercle

strong

regional

strong expression: see section 12 13 14 16 17 18 moderate expression: see section 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5098
Entity Detected:	Osr1, odd-skipped related 1 (Drosophila) ( MGI:1344424)
Sequence:	sense strand is shown >T5098 CCACGCGTCCGGTTCTCGCCGGCACTGGTTCAGATTCAGAAAGGGAGCCAGGAGCGAGGCTCAGCAGACC CCGGCGGGTAGAACTCCGGGGCTGTGAAGCGCTCCCGCGTGTCCGACTCTTGGAGTCACCGCAGCTACAG AAGCGTCAGAAGTCTAGTTCGCCAGGGGTGCCAGTCGGCTCCAGAGTCTCTGGTCACTCAAGTCCAGCGC AGCGACCCTCACAGACGCGCTCTGCCCTGGGACCGCGGCGGAACAAGATATTTGAAATCATTGCGGTTCC CAGCGACAGAAATGGGCAGCAAAACCTTGCCAGCACCGGTACCCATTCACCCATCCCTGCAGCTTACCAA TTACTCTTTCCTTCAGGCAGTGAATGGCCTGCCCACAGTGCCCTCGGACCACCTGCCCAACCTGTATGGT TTCAGCGCTCTTCATGCGGTGCATCTGCACCAATGGACGCTGGGCTACCCAGCCATGCACTTGCCTCGAT CCTCTTTTTCTAAAGTGCCAGGTGCGGTGTCCAGCCTGATGGACGCTCGCTTCCAGCTGCCAGCCTTCCC CTGGTTTCCCCACGTCATTCACCCTAAGCCAGAGATCACCGCGGGAGGCAGCGGCGCAGCACTTAAGACC AAGCCTCGCTTTGATTTCGCTAACCTGGCCTTGGCTGCCACACAAGAGGATCCCACCAAGCTTGGCCGAG GGGAGGGCCCTGGCTCCCCTGCTGGTGGGCTGGGCGCCCTCCTGGACGTGACCAAGCTATCCCCAGAAAA GAAGCCAACAAGGGGACGCCTGCCTTCCAAGACCAAGAAGGAATTCGTTTGCAAGTTCTGTGGCCGCCAC TTCACTAAGTCCTATAACCTACTTATCCACGAGCGGACGCACACTGATGAGCGACCTTACACCTGTGACA TCTGCCACAAAGCTTTCCGGAGGCAAGACCACCTACGGGACCACAGATATATTCACTCCAAAGAGAAGCC TTTCAAGTGTCAAGAGTGTGGGAAAGGATTCTGCCAGTCCCGGACTCTCGCTGTCCACAAGACGCTACAC TCACAAGTGAAGGAGCTCAAAACCTCCAAGATCAAATGCTAAATAAACAGAGCCTGCGGGGCGCCAGGAA CCTGGGGCGTGCTGCCGCCTTCTCTACCAGAGGGACCCAAGCTGAAGGCAACTCTGCGGGCAGCGGGAGG GGCTTTCGGGATCTTCCTTCCACAGCAACAAAATCCCTTGGGTCCTTTGCGCTTGCGGCGCTCCTGAGCC TTGGCCCGGGCCGTGACTCCTACAGCCTGGGCGGCTCCCCTAGGACGCGGCTAAAACTTGGCCAAAAGGC GGTACTCAACTCACACGCCGGAAGGAAAACTGCATTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323948 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323948 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002499 same experiment
	EMAGE:29539 same embryo
	EMAGE:29282 same embryo
	EMAGE:29395 same embryo
	EMAGE:29551 same embryo
	EMAGE:29279 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source