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EMAGE:31474

Ntng2 ( MGI:2159341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474
euxassay_014227_01 euxassay_014227_02 euxassay_014227_03 euxassay_014227_04 euxassay_014227_05
EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474
euxassay_014227_06 euxassay_014227_07 euxassay_014227_08 euxassay_014227_09 euxassay_014227_10
EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474
euxassay_014227_11 euxassay_014227_12 euxassay_014227_13 euxassay_014227_14 euxassay_014227_15
EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474 EMAGE:31474
euxassay_014227_16 euxassay_014227_17 euxassay_014227_18 euxassay_014227_19 euxassay_014227_20
EMAGE:31474 EMAGE:31474 EMAGE:31474
euxassay_014227_21 euxassay_014227_22 euxassay_014227_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
intermediate grey horn
weak weak
regionalweak expression: see section 11 12 13 14 15
vestibulocochlear viii ganglion
strong strong
single cellstrong expression: see section 20 21 moderate expression: see section 07 09 10 weak expression: see section 18 19
metanephros
strong strong
regionalstrong expression: see section 07 08 09 16 17 18
rest of cerebellum mantle layer
strong strong
regionalstrong expression: see section 08 09 17 18 moderate expression: see section 06
glossopharyngeal ix ganglion
moderate moderate
single cellmoderate expression: see section 09 weak expression: see section 18 19
pons mantle layer
strong strong
regionalstrong expression: see section 08 11 12 moderate expression: see section 07 10 16 17
cochlea
strong strong
regionalstrong expression: see section 22 weak expression: see section 04 05 06 08 09 10 20 21
hypothalamus mantle layer
strong strong
regionalstrong expression: see section 13 14 15 16 17
dorsal root ganglion
strong strong
single cellstrong expression: see section 09 10 17 18 moderate expression: see section 11 13 14 weak expression: see section 08 15 16
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 09 11 13 14 15 moderate expression: see section 16 17 18
trigeminal v ganglion
strong strong
single cellstrong expression: see section 09 10 17 20 21 22 moderate expression: see section 05 06 07 08 weak expression: see section 04 18 19
telencephalon mantle layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
midbrain mantle layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38709
Entity Detected:Ntng2, ( MGI:2159341)
Sequence:sense strand is shown

>T38709
GTAATGAGTGTGATGCCTCCAATCCCGACCTGGCCCACCCGCCCCGGCTTATGTTTGACAGGGAAGATGA
GGGACTGGCTACCTACTGGCAAAGCGTCACGTGGAGTCGCTACCCCAGTCCACTAGAGGCCAACATCACC
CTCTCATGGAACAAGAGCGTGGAGTTGACAGACGACGTGGTGGTGACTTTTGAGTATGGCCGGCCCACGG
TCATGGTCCTCGAGAAGTCCCTGGACAATGGCCGCACCTGGCAGCCCTACCAGTTCTATGCAGAGGACTG
CATGGAGGCCTTCGGCATGTCTGCCCGACGTGCCCGAGACATGTCACCCTCCAGCGCCCACCGGGTGCTC
TGCACCGAGGAGTACTCACGCTGGGCAGGGTCCAAGAAAGAGAAGCATGTGCGCTTTGAGGTAAGGGACC
GCTTTGCCATCTTTGCCGGCCCTGACCTGCGTAACATGGACAACCTGTACACGAGGATGGAGAGCGCCAA
GGGCCTCAAGGAGTTCTTCACCTTCACTGACCTGCGCATGCGCCTGCTGCGTCCTGCGCTGGGTGGCACC
TACGTGCAGCGAGAGAACCTCTACAAGTACTTCTATGCCATCTCCAATATCGAAGTCATTGGCAGGTGTA
AGTGCAACCTGCATGCCAACCTGTGCACAGTGCGAGAGGGCAGCCTGCAGTGTGAGTGTGAACACAACAC
CACGGGCCCCGACTGTGGCAGGTGCAAGAAGAACTTCCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104262. Forward Primer - name:104262_F_cDNA_Ntng2, sequence:GTAATGAGTGTGATGCCTCCAA; Reverse Primer - name:104262_N_SP6_cDNA_Ntng2, sequence:CGGAAGTTCTTCTTGCACCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_014227 same experiment
 EMAGE:30362 same embryo
 EMAGE:30183 same embryo
 EMAGE:30375 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS