EMAGE mouse gene expression spatial database

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EMAGE:31478

Atp2a1 ( MGI:105058)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005763_01	euxassay_005763_02	euxassay_005763_03	euxassay_005763_04	euxassay_005763_05

euxassay_005763_06	euxassay_005763_07	euxassay_005763_08	euxassay_005763_09	euxassay_005763_10

euxassay_005763_11	euxassay_005763_12	euxassay_005763_13	euxassay_005763_14	euxassay_005763_15

euxassay_005763_16	euxassay_005763_17	euxassay_005763_18	euxassay_005763_19	euxassay_005763_20

euxassay_005763_21	euxassay_005763_22	euxassay_005763_23	euxassay_005763_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 17 18 19 20 21 22 23 24

upper arm mesenchyme

moderate

regional

moderate expression: see section 01 02 03

foot

moderate

regional

moderate expression: see section 13 14 21 22

upper leg mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 20 21

vertebral axis musculature

strong

regional

strong expression: see section 17 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23 24

tail mesenchyme

strong

regional

strong expression: see section 17 moderate expression: see section 14 15 16 18 19 20

forearm mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

tongue

strong

regional

strong expression: see section 17 moderate expression: see section 13 14 weak expression: see section 15 16 18 19

lower leg mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7351
Entity Detected:	Atp2a1, ( MGI:105058)
Sequence:	sense strand is shown >T7351 CGTGGAAAGGAGAAGAATCTGGGGGGGGCAAGCAAGAAAAAGGAAGGAGCCCAGATCAACAGGCAGGTGG ATAAGCTGAAGAAGTCCCCCCACAAGTGAGAGGCCCCTAAGAAGAAACACATCACCCCCTGGCCCCCAGG AAGGGAGCACAATGGAGGCCGCGCACTCCAAGTCCACAGAGGAATGTTTGTCCTATTTCGGGGTGAGCGA GACCACAGGCCTTACCCCAGACCAAGTTAAGCGACATCTGGAAAAATACGGCCCCAATGAGCTCCCTGCT GAGGAAGGCAAGTCTCTGTGGGAGTTGGTGGTAGAGCAGTTCGAAGACCTGCTTGTGCGGATTCTTCTCC TGGCTGCCTGCATTTCCTTTGTGCTGGCCTGGTTTGAGGAAGGCGAAGAAACCGTCACTGCCTTTGTGGA ACCTTTTGTCATTCTCTTGATCCTCATTGCCAACGCCATTGTGGGGGTTTGGCAGGAACGGAATGCAGAG AACGCTATCGAAGCGCTGAAAGAATATGAACCCGAGATGGGAAAGGTCTATCGGGCTGACCGAAAGTCAG TGCAAAGGATCAAGGCTCGGGACATCGTTCCTGGGGACATCGTGGAGGTGGCCGTTGGGGACAAAGTCCC TGCAGACATCCGCATCCTCTCCATCAAATCCACCACCCTCCGGGTCGACCAGTCCATCCTGACAGGAGAG TCTGTGTCTGTCATCAAGCACACAGACCCTGTCCCTGACCCCCGAGCTGTCAACCAGGACAAGAAGAACA TGCTCTTCTCAGGTACCAACATCGCAGCCGGCAAGGCTGTAGGCATCGTGGCCACCACTGGTGTGAGTAC TGAGATTGGCAAGATCCGAGACCAGATGGCTGCCACCGAGCAGGACAAGACTCCACTGCAGCAGAAGCTT GACGAGTTTGGGGAGCAGCTGTCCAAGGTCATCTCTCTCATCTGTGTGGCTGTCTGGCTTATCAACATTG GCCACTTCAACGATCCTGTCCATGGGGGCTCCTGGTTCCGGGGTGCCATTTACTACTTCAAGATTGCTGT GGCCTTGGCTGTGGCGGCCATCCCAGAAGGGCTTCCTGCTGTCATCACCACCTGCTTGGCTTTGGGTACC CGCCGGATGGCAAAGAAGAACGCCATCGTGAGGAGTCTGCCCTCTGTGGAGACCCTGGGCTGTACCTCTG TCATCTGTTCTGACAAAACAGGGACCCTCACCACCAACCAGATGTCAGTTTGCAAGATGTTCATCATTGA CAAGGTGGATGGGGATGTCTGCTCACTGAACGAGTTCTCCATCACTGGCTCTACTTATGCTCCTGAGGGA GAGGTCTTGAAGAATGATAAGCCAGTCCGAGCAGGGCAGTATGATGGGCTGGTGGAGCTCGCTACCATCT GTGCCCTCTGCAATGATTCCTCCTTGGACTTTAATGAGACCAAAGGTGTCTATGAGAAGGTGGGCGAGGC CACGGAGACTGCTCTCACCACCTTGGTGGAGAAGATGAACGTATTCAACACGGAAGTGAGAAGCCTCTCT AAAGTGGAGCGGGCCAACGCCTGCAATTCGGTGATCCGCCAACTCATGAAGAAGGAATTCACCCTGGAAT TTTCCCGAGACAGGAAGTCTATGTCTGTCTACTGTTCACCAGCTAAGTCCTCACGGGCTGCCGTGGGCAA CAAGATGTTTGTCAAGGGTGCCCCCGAGGGGGTCATCGACCGCTGTAACTATGTGCGTGTTGGCACGACC CGTGTGCCATTGACAGGCCCCGTGAAGGAGAAGATCATGTCTGTGATCAAGGAGTGGGGCACTGGTCGGG ACACACTGCGGTGCCTGGCGCTGGCCACCCGCGACACGCCCCCCAAACGCGAGGAGATGGTTCTGGATGA TTCAGCCAAGTTCATGGAGTATGAGATGGACCTGACGTTTGTTGGTGTTGTGGGCATGTTGGATCCACCC CGCAAGGAGGTCACAGGCTCTATCCAGCTGTGTCGTGATGCTGGGATCCGAGTGATCATGATCACTGGGG ACAACAAGGGTACAGCCATTGCCATCTGCCGACGAATTGGCATCTTTTCGGAGAATGAGGAAGTGACAGA CCGTGCCTACACTGGCCGTGAGTTTGACGATCTGCCCCTGGCCGAGCAGCGGGAGGCCTGCAGACGCGCC TGCTGCTTTGCTCGTGTGGAGCCCTCACACAAGTCCAAGATTGTGGAGTACCTGCAGTCCTACGATGAGA TCACAGCCATGACAGGGGATGGTGTCAATGATGCCCCTGCTCTGAAGAAGGCCGAGATTGGCATTGCCAT GGGATCTGGTACTGCTGTGGCTAAGACGGCCTCTGAAATGGTACTGGCCGATGATAACTTCTCCACCATC GTGGCTGCCGTGGAGGAAGGCCGCGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCT CCAATGTGGGCGAGGTGGTCTGTATCTTCTTGACAGCAGCCTTGGGGCTGCCTGAGGCTCTGATCCCTGT GCAGCTGCTCTGGGTGAACTTGGTGACTGATGGGCTCCCGGCTACTGCCCTGGGATTCAACCCACCTGAC CTGGACATCATGGACCGCCCCCCCAGGAGTCCCAAGGAGCCTCTTATCAGTGGCTGGCTCTTTTTCCGCT ACATGGCAATTGGGGGCTATGTGGGTGCAGCCACTGTAGGAGCAGCTGCCTGGTGGTTTTTGTATGCAGA GGACGGGCCTCATGTCAGCTACCATCAGCTGACTCATTTCATGCAGTGCACTGAGCACAACCCTGAATTC GATGGCCTGGACTGTGAGGTCTTTGAGGCCCCCGAGCCCATGACCATGGCCTTGTCTGTGTTGGTGACCA TCGAGATGTGCAATGCTCTCAACAGCCTGTCTGAGAACCAGTCCCTACTGCGGATGCCACCCTGGGTGAA CATCTGGCTGCTGGGTTCCATCTGCCTGTCCATGTCCCTCCACTTCCTCATCCTCTATGTCGACCCCCTG CCGATGATCTTCAAGCTCCGGGCCCTGGACTTTACCCAGTGGCTCATGGTCCTCAAGATCTCACTGCCAG TCATCGGGCTGGATGAGCTTCTCAAGTTCATTGCTCGGAACTATCTGGAGGGATAACCACCCCCCTCCTC CATGTCTTTGAACCGTGTCACAGATCCAGAAGACGAAAGAAGGAAGTAACCAGCCCTTGTGCCCTGTGTT CCCCACCCCAATAGCCATGCATCTTCAGCCAAAGCTGAGGCACTGACCCTTCTTCCCCCTCAATTCTGTT TATAAATAAAGTCCTCCACCCTCGTCCCCTTCAGCAACCCGCTCTCCCTCCGAACCTTGTAAATCCCCTT CCCGAACTCACGGGGCTTGCAGGCACAAGGCAACTGACTAGCTGATTGCTTATTTATTGAAAATAAATGA CAGAAAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191741 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191741 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005763 same experiment
	EMAGE:30394 same embryo
	EMAGE:30383 same embryo
	EMAGE:30360 same embryo
	EMAGE:30370 same embryo
	EMAGE:31486 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source