EMAGE mouse gene expression spatial database

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EMAGE:31495

Gpr19 ( MGI:892973)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011984_01	euxassay_011984_02	euxassay_011984_03	euxassay_011984_04	euxassay_011984_05

euxassay_011984_06	euxassay_011984_07	euxassay_011984_08	euxassay_011984_09	euxassay_011984_10

euxassay_011984_11	euxassay_011984_12	euxassay_011984_13	euxassay_011984_14	euxassay_011984_15

euxassay_011984_16	euxassay_011984_17	euxassay_011984_18	euxassay_011984_19	euxassay_011984_20

euxassay_011984_21	euxassay_011984_22	euxassay_011984_23	euxassay_011984_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 04 17 18 19 20 21 22 23

vault of skull

moderate

homogeneous

moderate expression: see section 01

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 04 20 21 22 23 24

fibula

moderate

homogeneous

moderate expression: see section 01 02 03 23 24

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 09 16 17 weak expression: see section 08

rib

moderate

homogeneous

moderate expression: see section 01 02 20 21 22 23 weak expression: see section 03 04 05 06 07 08 09 14 15 16 17 18 19

axial skeleton

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15

nose

moderate

homogeneous

moderate expression: see section 08 09 10 18 19 20

foot

moderate

homogeneous

moderate expression: see section 05

meckel's cartilage

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 19 20 21 weak expression: see section 12

basioccipital bone

moderate

homogeneous

moderate expression: see section 01 02 03 04 17 18 19 20 21 22 23 24

basisphenoid bone

moderate

homogeneous

moderate expression: see section 09 13 14 15 weak expression: see section 10 11

tibia

moderate

homogeneous

moderate expression: see section 01 02 03 23 24

scapula

moderate

homogeneous

moderate expression: see section 01 02 03 04 20

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22 23 24 weak expression: see section 08

naris

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 16

nasal septum

moderate

homogeneous

moderate expression: see section 14 15

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 07 08 09 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30071
Entity Detected:	Gpr19, ( MGI:892973)
Sequence:	sense strand is shown >T30071 CACCGCCAGCATCTTCTTTGGCGCTTTGTGGTTGTTCTCCATCTTTGGCAATTCCCTGGTATGTCTGGTC ATCCACCGGAGCCGGAGGACTCAGTCCACCACCAACTACTTCGTGGTGTCCATGGCGTGTGCTGACCTTC TCATCAGCGTGGCCAGCACGCCGTTTGTCGTGCTGCAGTTCACCACCGGGAGGTGGACCCTGGGTAGCGC CATGTGCAAGGTGGTCCGCTACTTCCAGTACCTCACCCCCGGCGTGCAGATCTACGTGCTGCTCTCCATC TGCATCGACCGCTTCTATACCATCGTCTACCCGCTGAGCTTCAAGGTGTCCCGAGAGAAGGCCAAGAAAA TGATTGCAGCCTCCTGGATCTTGGACGCGGCCTTCGTGACGCCTGTCTTCTTTTTCTACGGCTCTAACTG GGATAGTCACTGTAACTACTTCCTCCCTCCCTCCTGGGAGGGAACTGCCTATACCGTCATCCACTTCTTG GTGGGCTTCGTGATTCCCTCCATCCTCATAATCCTGTTTTACCAGAAGGTCATAAAGTATATCTGGAGAA TAGGCACGGACGGGCGGACGCTGAGAAGGACGATGAACATTGTCCCCAGGACAAAGGTGAAAACGGTCAA GATGTTCCTGCTCTTGAACCTAGTGTTCCTGTTCTCCTGGCTACCTTTCCATGTGGCTCAGCTCTGGCAT CCCCATGAGCAAGACTACAAGAAGAGCTCCCTTGTCTTCACGGCAGTCACGTGGGTATCTTTCAGCTCTT CAGCCTCGAAACCCACTCTGTACTCTATTTATAATGCCAATTTTCGGAGAGGGATGAAAGAGACATTCTG CATGTCCTCGATGAAATGCTACCGCAGCAATGCCTACACCATCACGACCAGCTCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3989077), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17225. Forward Primer - name:017225_F_IRAV20-23_D15_Gpr19, sequence:CACCGCCAGCATCTTCTT; Reverse Primer - name:017225_R_SP6_IRAV20-23_D15_Gpr19, sequence:CGTGAGCTGGTCGTGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011984 same experiment
	EMAGE:31209 same embryo
	EMAGE:31494 same embryo
	EMAGE:31890 same embryo
	EMAGE:31498 same embryo
	EMAGE:31497 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source