Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31498

Mia1 ( MGI:109615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498
euxassay_011988_01 euxassay_011988_02 euxassay_011988_03 euxassay_011988_04 euxassay_011988_05
EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498
euxassay_011988_06 euxassay_011988_07 euxassay_011988_08 euxassay_011988_09 euxassay_011988_10
EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498
euxassay_011988_11 euxassay_011988_12 euxassay_011988_13 euxassay_011988_14 euxassay_011988_15
EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498
euxassay_011988_16 euxassay_011988_17 euxassay_011988_18 euxassay_011988_19 euxassay_011988_20
EMAGE:31498 EMAGE:31498 EMAGE:31498 EMAGE:31498
euxassay_011988_21 euxassay_011988_22 euxassay_011988_23 euxassay_011988_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 5 mesenchyme
moderate moderate
regionalmoderate expression: see section 05 23
sternum
moderate moderate
regionalmoderate expression: see section 12 13
radius
moderate moderate
regionalmoderate expression: see section 01
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 04 19 20 21 23 24
hindlimb digit 4 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 23 24
clavicle
moderate moderate
regionalmoderate expression: see section 05 08 09
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 08 09 10 16 17
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16
tarsus
moderate moderate
regionalmoderate expression: see section 23
nose
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 17 18 19 20 21
otic capsule
moderate moderate
regionalmoderate expression: see section 07 08 09 15 16
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 23 24
tibia
moderate moderate
regionalmoderate expression: see section 01 02 03 23 24
femur
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 08 18 19 20 21 23 24
naris
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18
hand
moderate moderate
regionalmoderate expression: see section 24
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 20 21 22 23 24
cricoid cartilage
moderate moderate
regionalmoderate expression: see section 12 13
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 18 19 20 21 22 23
hindlimb digit 1 mesenchyme
moderate moderate
regionalmoderate expression: see section 05 23
thyroid cartilage
moderate moderate
regionalmoderate expression: see section 12 13
vault of skull
moderate moderate
regionalmoderate expression: see section 01 02 03
fibula
moderate moderate
regionalmoderate expression: see section 02 03 23 24
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 05 23 24
hyoid
moderate moderate
regionalmoderate expression: see section 15 16
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
basioccipital bone
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21 22 23 24
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15
naso-lacrimal duct
moderate moderate
regionalmoderate expression: see section 15
scapula
moderate moderate
regionalmoderate expression: see section 02 03 04 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30069
Entity Detected:Mia1, ( MGI:109615)
Sequence:sense strand is shown

>T30069
GTGTGGTCCCCAGTGCTCCTTGGCATCGTCGTCTTGTCTGTTTTTTCAGGGCCTAGCAGGGCTGATCGAG
CTATGCCCAAGCTGGCTGACTGGAAGCTGTGTGCGGACGAGGAATGCAGCCATCCTATCTCCATGGCTGT
GGCCCTCCAGGACTACGTGGCCCCTGATTGCCGCTTCTTGACTATATATAGGGGCCAAGTGGTGTATGTC
TTCTCCAAGTTGAAGGGCCGTGGGCGCCTTTTCTGGGGAGGCAGTGTGAGTCTTCAGAGAGTGATAATGG
GAAGGGTGTGGGGTTTGTTTGGGTTTGTAAAGCCTTGTTTTCCATATGAAGGGAAGATTTGGGGGGAGGG
GAAAACGAGATTTGGGGGAAGGAAGGACATTTTATTACTTATTGCATTTTTTTTTCTTTAGAAATTACAG
AGAAGCTTAGCGCCAAAGCTCTTTTTCATACCCGGGATACAACAGTGAACAAAGGGGTAGAGGCAGGCAA
CCAGCAAGCCATCAAAAGTGGCAGCAAACATGACATTGTGAGGATTAGCTGTAGTGGCAACATTCTATCC
ATGAGCAAATGGCATTTAAGCTGAGATTCAAGTAAGAGGGAAGATTGTACACAGGCGTTCAGCAAGCATA
AGTGCCGTTTCCCCAGATGGACTTTTAACTTCTTCTCCAGGTTCAGGGAGGTTACTATGGAGACCTGGCA
GCCCGCCTGGGCTATTTCCCCAGTAGCATTGTCCGGGAGGACCTGACTCTGAAACCTGGCAAAATTGATA
TGAAGACAGATCAATGGGATTTCTACTGCCAGTGAGCTCAGCCTACCGCTATCCCTGCAGTTACCCTTCC
GGCTCTATGCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3987973), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17045. Forward Primer - name:017045_F_IRAV20-23_D11_Mia, sequence:GTGTGGTCCCCAGTGCTC; Reverse Primer - name:017045_R_SP6_IRAV20-23_D11_Mia, sequence:TTGCATAGAGCCGGAAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_011988 same experiment
 EMAGE:31209 same embryo
 EMAGE:31495 same embryo
 EMAGE:31494 same embryo
 EMAGE:31890 same embryo
 EMAGE:31497 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS