EMAGE mouse gene expression spatial database

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EMAGE:31502

Farslb ( MGI:1346035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006146_01	euxassay_006146_02	euxassay_006146_03	euxassay_006146_04	euxassay_006146_05

euxassay_006146_06	euxassay_006146_07	euxassay_006146_08	euxassay_006146_09	euxassay_006146_10

euxassay_006146_11	euxassay_006146_12	euxassay_006146_13	euxassay_006146_14	euxassay_006146_15

euxassay_006146_16	euxassay_006146_17	euxassay_006146_18	euxassay_006146_19	euxassay_006146_20

euxassay_006146_21	euxassay_006146_22	euxassay_006146_23	euxassay_006146_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cranial musculature

strong

regional

strong expression: see section 17 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23 24

stomach

moderate

regional

moderate expression: see section 06 08

metanephros

moderate

regional

moderate expression: see section 04 05

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 05

trigeminal v nerve

moderate

regional

moderate expression: see section 09

axial musculature

strong

regional

strong expression: see section 01 02 03 04 05 17 18 19 20 21 moderate expression: see section 14 15 16

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 10 11 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 18 moderate expression: see section 06

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

trigeminal v ganglion

strong

regional

strong expression: see section 17 18 moderate expression: see section 05 06 07 08 16 19 20 21 weak expression: see section 02 03 04

renal cortex

moderate

regional

moderate expression: see section 06 07 08 09 14 15 16 17 18 19

vibrissa

moderate

regional

moderate expression: see section 08 09 10 11 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 12 13 17 18 moderate expression: see section 11 14 15 16 19 20

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7534
Entity Detected:	Farslb, ( MGI:1346035)
Sequence:	sense strand is shown >T7534 GGCGCAGTGTCCACGACTCGCCATGCCGACTGTCAGCGTAAAGCGAGACCTGCTCTTCCAAGCCCTGGGC CGGACCTACACTGATGAAGAGTTTGACGAACTGTGTTTTGAATTTGGTCTGGAACTTGATGAAATTACTT CTGAGAAGCAAATCATAAGCAAGGAACAAGGTCATGGGAAGGCACAGGGGGCCTCAGATGTTGTGCTTTA CAAGATTGACGTCCCTGCCAACAGATACGACCTCCTGTGTCTGGAAGGACTGGCTCGGGGCCTCCAGGTC TTCAAAGAAAGGATCAAAGCTCCGGTATATAAGCGAGTGATGCCCAAGGGAGACATCCAAAAGCTGGTTA TCACAGAAGAGACAGCCAAAGTGCGCCCTTTTGCTGTAGCAGCAGTTCTCCGGAATATCAAGTTCACTAA AGACCGATATGACAGCTTCATTGAACTTCAGGAGAAACTACACCAGAATATCTGCAGGAAAAGAGCGTTG GTTGCTATTGGAACTCACGACTTGGACACTTTATCAGGCCCATTTACTTACACCGCGAAACGTCCTTCAG ATATCAAATTCAAGCCTCTGAATAAGACCAAGGAGTATACAGCTTGTGAATTAATGAACATATACAAGAC TGACAACCACCTTAAACATTATTTACATATCATTGAAAGTAAGCCCCTGTATCCAGTTATCTATGACAGC AATGGTGTGGTCCTGTCGATGCCTCCGATTATAAATGGGAATCATTCAAAAATAACAGTAAATACTAGGA ATATATTTATTGAATGCACAGGGACTGACTTCACTAAGGCAAAAATCGTGCTTGACATTATTGTCACCAT GTTCAGTGAACACTGTGAAAATCAGTTTACAGTTGAAGCAGTTGAAGTAGTTTCTCCTAATGGAAAATCA AGTACCTTTCCAGAGCTGCCTTACAGAAAGGAGATGGTGAGAGCTGATTTAATTAACAAGAAAGTTGGAA TCAGAGAAACTCCTGCAAATCTCGCCAAGCTCCTGACCAGGATGTGTCTGAAGTCAGAAGTCATAGGTGA CGGCAATCAGATTGAGGTTGAAATTCCTCCGACCAGAGCAGATGTCATCCATGCATGTGACATTGTGGAG GACGCAGCTATTGCTTATGGCTATAACAACATTCAGATGACTCTGCCGAAAACGTACACCATAGCTAATC AATTTCCTCTCAACAAGCTCACCGAGCTCCTCAGACTTGACATGGCTGCGGCTGGCTTCACCGAAGCACT GACTTTTGCTCTGTGCTCCCAAGAAGATATTGCTGACAAGCTTGGCCTGGATATCTCTGCAACAAAGGCA GTGCACATAAGTAATCCCAAAACCGCTGAGTTCCAGGTGGCGCGCACTACCCTTCTTCCTGGCCTTCTAA AGACCATTGCAGCAAACCGGAAGATGCCCCTTCCTCTCAAACTGTTTGAAATCTCTGACGTTGTAGTAAA AGATTCTGGCAAAGATGTAGGTGCGAAAAACTACAGGCATCTCTGTGCTGTTTATTACAGCAAGACTCCC GGGTTTGAGATCATCCATGGCTTGCTGGACAGAATTATGCAGCTGCTCGATGTGCCTCCTGGTGAGGAAA GCGGGGGCTACATGATCAAAGCGTCTGCAGGTTCTGCATTTTTCCCTGGACGGTGTGCTGAGATCTTTGT TGGGGGTCAAAGCATTGGGAAGCTAGGCGTCCTTCATCCTGATGTTATCACCAAATTTGAGCTGACCATG CCCTGTTCCTCTCTGGAAATCAATATTGAGCCCTTTTTGTGAGGTTGGTCTCTGAGTGTCCCTTTCTGCT CCTGTGGTGTCCTTCAGCCCTCTTTTACAGCACATATCCATTTGTGCGTTAATGTTTAATAAAGTGAAAT GTGCTATCTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239906 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239906 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006146 same experiment
	EMAGE:31895 same embryo
	EMAGE:31785 same embryo
	EMAGE:29950 same embryo
	EMAGE:31535 same embryo
	EMAGE:31897 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source