EMAGE mouse gene expression spatial database

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EMAGE:31503

2810002O09Rik ( MGI:1919595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006388_01	euxassay_006388_02	euxassay_006388_03	euxassay_006388_04	euxassay_006388_05

euxassay_006388_06	euxassay_006388_07	euxassay_006388_08	euxassay_006388_09	euxassay_006388_10

euxassay_006388_11	euxassay_006388_12	euxassay_006388_13	euxassay_006388_14	euxassay_006388_15

euxassay_006388_16	euxassay_006388_17	euxassay_006388_18	euxassay_006388_19	euxassay_006388_20

euxassay_006388_21	euxassay_006388_22	euxassay_006388_23	euxassay_006388_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 15 16

anterior abdominal wall musculature

weak

regional

weak expression: see section 05 06 07 08 16 17 18 19

physiological umbilical hernia

weak

regional

weak expression: see section 11 12 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 16 17 18 19 weak expression: see section 09 10 11 12 13 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35087
Entity Detected:	2810002O09Rik, ( MGI:1919595)
Sequence:	sense strand is shown >T35087 AAAGGAGCATCAACTTCTAGCGATGCCCAAGACCAGGGAGCTGAAAAGGGAGCCAAGAATAAGACAACTG AGGCAACAGAAGGACCAACATCAGAGCCTCCCTTATCAGGCCCAGGTAGGCTGAAGAAAACTGCCATGAA ACTCTTTGGTGGCAAGAAGGGAATCTGTACTCTGCCTAGTTTCTTTGGGGGTGGACGAAGCAAAGGTTCT GGGAAAGTCAGCTCTAAGAAAAGTCTAAACAAGAGTAAGACTCACGATGGTCTAAGTGAAGCGTCTCAGG GACCTGAAGATGTTGTCATTGAAGAAACAGACCTCTCTACACCTTTGTCCAAGTCATCTGCTCAATTTCC TAGCTCCCAGAGTGCCAATGGAGCGTTGGAGATAGGCTCTAAACATAAGACATCTGGGACTGAAGCCATA GAAAAAGCTGGAGTTGAGAAGGTACCCTCTGTGCACAAGCCGAAGAAAAGCCTAAAAAGCTTCTTTAGCA GTATCCGCCGTCACCGGAAGGGCAAGACTTCTGGGGCTGACCAAAGTGTGCCAGGGGCCAAGGAACTTGA GGGGGCAAGAACCAGGTCTCATGAGCATGTGAGTTCAATCTCTCTGCCCAGCTCTGAGGAGATATTCCGA GACACTAGAAAGGAAAATGCCAAACCCCAGGATGCCCCGGACCAAAAATGTCTCCAGCACAAGTACATTT TTCACCAACTACTGAGAAGGCAGCTTGTAAAAATCCAGAAAAACTCACAAGGACCTGTGCCTCAGAGTTC ATGCAGCCCAAGCCTGTTTTTGAAGGTGGTAGTCTAGAGGAGCCCCCACAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68227. Forward Primer - name:068227_F_cDNA_2810002O09Rik, sequence:AAAGGAGCATCAACTTCTAGCG; Reverse Primer - name:068227_N_SP6_cDNA_2810002O09Rik, sequence:GTGTGGGGGCTCCTCTAGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006388 same experiment
	EMAGE:29270 same embryo
	EMAGE:29273 same embryo
	EMAGE:29276 same embryo
	EMAGE:29268 same embryo
	EMAGE:29504 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source