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EMAGE:31514

Msln ( MGI:1888992)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514
euxassay_001942_01 euxassay_001942_02 euxassay_001942_03 euxassay_001942_04 euxassay_001942_05
EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514
euxassay_001942_06 euxassay_001942_07 euxassay_001942_08 euxassay_001942_09 euxassay_001942_10
EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514
euxassay_001942_11 euxassay_001942_12 euxassay_001942_13 euxassay_001942_14 euxassay_001942_15
EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514 EMAGE:31514
euxassay_001942_16 euxassay_001942_17 euxassay_001942_18 euxassay_001942_19 euxassay_001942_20
EMAGE:31514 EMAGE:31514
euxassay_001942_21 euxassay_001942_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
diaphragm
moderate moderate
regionalmoderate expression: see section 18 19 20 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
trachea
moderate moderate
regionalmoderate expression: see section 10 11
hindgut
strong strong
regionalstrong expression: see section 07 09
foregut-midgut junction
strong strong
regionalstrong expression: see section 07 08 09 10
heart ventricle
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14
stomach
strong strong
regionalstrong expression: see section 02 03 04 05
rectum
strong strong
regionalstrong expression: see section 08
midgut
strong strong
regionalstrong expression: see section 07 08 09 10 11
bladder
strong strong
regionalstrong expression: see section 08 09
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T680
Entity Detected:Msln, ( MGI:1888992)
Sequence:sense strand is shown

>T680
TCCTCGAGNCTGTTGGCCTACTGGACTACTGCAGGTGCTCACAGAAGTTGGACAGCTGCTTTCCCAGGCC
CAAAAGCCCCTTCGTTGTCTCCAAACAGTGGTGTGGGTTGAGGGGTGGGACAAGTGGGGACCTCAGAGTC
ATTGTTATCCACAGACCATGGCCTTGCCAACAGCTCGACCCCTGCTGGGGTCCTGTGGAAGTCCCATCTG
CAGCCGAAGCTTCCTACTGCTTCTCCTTAGTCTTGGGTGGATACCACGTCTGCAGACCCAGACTACAAAG
ACAAGCCAGGAGGCCACACTCCTCCATGCTGTGAACGGTGCCGCTGACTTTGCCAGTCTCCCCACAGGCC
TCTTTCTTGGCCTCACATGTGAGGAGGTATCTGACCTGAGCATGGAACAAGCCAAGGGGCTGGCTATGGC
TGTAAGACAGAAGAACATTACACTCCGGGGACATCAGCTGCGTTGTCTGGCACGTCGCCTTCCTAGGCAC
CTCACCGACGAGGAACTGAATGCTCTTCCACTGGACCTGCTGCTCTTCCTCAACCCAG
Notes:The probe template was PCR amplified from IMAGE:1888713 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1888713 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001942 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS