EMAGE mouse gene expression spatial database

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EMAGE:31534

BC037034 ( MGI:2385896)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007006_01	euxassay_007006_02	euxassay_007006_03	euxassay_007006_04	euxassay_007006_05

euxassay_007006_06	euxassay_007006_07	euxassay_007006_08	euxassay_007006_09	euxassay_007006_10

euxassay_007006_11	euxassay_007006_12	euxassay_007006_13	euxassay_007006_14	euxassay_007006_15

euxassay_007006_16	euxassay_007006_17	euxassay_007006_18	euxassay_007006_19	euxassay_007006_20

euxassay_007006_21	euxassay_007006_22	euxassay_007006_23	euxassay_007006_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 03 04 23 24 moderate expression: see section 01 02

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 18 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13 16 17

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v nerve

strong

regional

strong expression: see section 17 moderate expression: see section 10 11

thoracic ganglion

moderate

regional

moderate expression: see section 13 15 17

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 09 18

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

cervical ganglion

moderate

regional

moderate expression: see section 10 17 18

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4282
Entity Detected:	BC037034, ( MGI:2385896)
Sequence:	sense strand is shown >T4282 CCACGCGTCCGCACCCCTTTCATATAACCCCTAGCTGCCTGTGGAAGAACCGATTGTGTCCACAGATGAA GTCATCTTCCCACTCACCGTTTCACTGGATAGACTGCCCCCAGGGACACCCAAGGCCAAGATTGTAGTGA CCGTGTGGAAGCGGGAGGTCGAGGCACCAGAAGTCAGAGATCAAGGCTACCTGCGCTTGCTACAGACTCG ATCTCCTGGGGAGACTTTCCGTGGAGAACAGAGCGCCTTCAAGGCCCAAGTGAGCACCCTATTGACGCTG CTGCCCCCTCCAGTTCTGAAATGCCGGCAGTTCACTGTGGCTGGAAAGCACTTGACCGTGCTCAAGGTGC TGAATAGCTCTTCCCAGGAAGAGATCTCCATCTGGGATATCCGAATCCTCCCCAACTTCAATGCCAGTTA TCTACCTGTCATGCCTGATGGCTCTGTGCTGCTGGTGGACAATGTCTGTCACCAGTCTGGGGAAGTCTCC ATGGGATCCTTCTGTCGGCTTCCTGGAACATCTGGCTACTTCCCCTGTCCACTTAGTGCCCTGGAGGAAC ACAACTTCCTGTTCCAGCTGAGAGGGGGTGAGCAGCCCCCTCCTGGGGCCAAGGAGGGCCTTGAAGTCCC CTTGATTGCCGTGGTTCAGTGGTCCACGCCGAAGCTGCCCTTCACCCAGAGCATTTACACCCACTACCGC CTGCCCAGTGTCCGCCTAGACCGCCCCTGCTTTGTGATGACTGCTTCTTGTGAGTCCCCTGTTCGGACCT ACGAGCGTTTCACTGTCACCTACACGCTGCTCAACAACCTCCAAGACTTCCTTGCTGTGAGGCTTGTGTG GACCCCGGAACATGCACAGGCTGGAAAGCAGCTGTGTGAGGAGGAGCGCCGGGCCATGCAGGCAGCCCTA GACTCCATCGTCTGTCACACACCCCTCAATAATCTTGGCTTCTCCCGGAAGGGCAGCGCGCTTACCTTCA GTGTGGCCTTCCAGGCCCTGAGGACGGGGCTCTTTGAGCTGAGCCAGCACATGAAACTGAAGCTGCAGTT CACTGCCAGCGTGTCCCACCCTCCGCCTGAAGCCCGGCCCCTGTCCCGAAAGAGCAGCCCCAGCAGTCCT GCTGTCCGAGACCTGGTGGAGAGACACCAGGCCAGCCTGGGCCGTTCTCAATCTTTCTCCCACCAGCAGC CTTCCCGTAGCCACCTCATGAGGTCAGGCAGTGTGATGGAACGCAGGGCCATCACACCCCCTGTGGCCTC TCCCGTCGGCCGTCCCCTCTACCTGCCCCCCGACAAGGCTGTGCTTTCTCTTGATAAGATTGCCAAGCGT GAGTGTAAAGTCCTGGTGGTGGAGCCCGTCAAGTAGCACCGTTTGCTAGCTCAACCCCGGACAGGACACG CCAGAAACCCTAAGCCCCAGAGGGTCCCTTGTTCCTAGGCTGTACCTCCCACTGTGGGCAGAGAGGCCTT GCAGGGCTGCCTGTCTGTGTCTGCTGGTGAGGGATGAGGGCAGAAGCTGTGAAATCACAGTGTGGCTGCA GCTCAGACAACAGTATTCCATAAGTTCGGGGGGGGGGGGGTGCGCGAGGGGAGGGCTGGCCCCACCCCAG CAGGGCAAGGGCTGCGGGAGCTCCCTTGTCCTGTCCTTCGTCCAGTTTACAGAGTATCCTCTCTCACTCG TGTTCAAGCCTCCCTGCCACTGCCGCTGCTGTTCCTTTGCACTCACCCGCAGGGCTGAGATTAGAGGGTG GTAGTGGTAAAGGGACCCAGGACAATGACTCTCCTGTCTCAGGGGTTGGATGGCCTCTTTCTGCCCCTCA TGTTTACGTTGGCAGCCTGAAGCACTTTAAGTTCTGTTTTTGTTTTCTGTTTTTGTCTGTTCCTGTAACT TATTCTCTGACTACATCTTCCAGCTGAAGTAAAACTATTAAAAGCTGTTTGGGAGGGAGAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:4505093 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4505093 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007006 same experiment
	EMAGE:30160 same embryo
	EMAGE:31849 same embryo
	EMAGE:31750 same embryo
	EMAGE:29579 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source