EMAGE mouse gene expression spatial database

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EMAGE:31541

Bgn biglycan ( MGI:88158)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005068_01	euxassay_005068_02	euxassay_005068_03	euxassay_005068_04	euxassay_005068_05

euxassay_005068_06	euxassay_005068_07	euxassay_005068_08	euxassay_005068_09	euxassay_005068_10

euxassay_005068_11	euxassay_005068_12	euxassay_005068_13	euxassay_005068_14	euxassay_005068_15

euxassay_005068_16	euxassay_005068_17	euxassay_005068_18	euxassay_005068_19	euxassay_005068_20

euxassay_005068_21	euxassay_005068_22	euxassay_005068_23	euxassay_005068_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 11 12 moderate expression: see section 06 07 08 09 10 13 14 15 16 17 18

spinal cord meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14

lateral ventricle choroid plexus

strong

regional

strong expression: see section 11 moderate expression: see section 07 08 09 10 16 17 18 20 21

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7489
Entity Detected:	Bgn, biglycan ( MGI:88158)
Sequence:	sense strand is shown >T7489 CGGACGCGTGGGCGAACTGCCCAGGAGCGAGCAGCTGCTCCCGGTTGGCCCTGACGGACAGACAAACCGA CAGCCTGACAACCTAGTCCACCAACTAAGCAGCCTGCACCTGGCTGCTTGTCCCTCCCCAGGAACATTGA CCATGTGTCCCCTGTGGCTACTCACCTTGCTGCTGGCCCTGAGCCAGGCCTTGCCCTTTGAGCAGAAGGG TTTCTGGGACTTCACCTTGGATGATGGGCTGCTCATGATGAATGATGAGGAGGCTTCAGGTTCAGACACC ACTTCAGGTGTCCCCGACCTGGACTCTGTCACACCTACCTTCAGTGCCATGTGTCCTTTCGGTTGCCACT GCCACCTGCGGGTTGTTCAGTGCTCTGACTTGGGTCTGAAGACTGTGCCCAAGGAGATCTCACCTGACAC CACACTGCTAGACCTGCAGAACAATGACATTTCTGAGCTTCGCAAGGATGACTTCAAAGGCCTCCAGCAC CTCTACGCCCTGGTCTTGGTAAACAATAAGATCTCCAAGATCCATGAGAAGGCCTTTAGCCCTCTGCGGA AGCTGCAAAAACTCTACATCTCCAAGAACCACCTGGTGGAGATTCCTCCCAACCTGCCCAGCTCCCTGGT AGAACTACGAATCCATGACAACCGTATCCGCAAAGTGCCCAAGGGCGTGTTCAGCGGGCTCCGGAACATG AACTGCATTGAGATGGGCGGGAATCCCCTGGAGAACAGTGGCTTTGAACCAGGAGCCTTTGATGGCCTGA AGCTCAATTACCTGCGCATCTCAGAGGCCAAGCTCACTGGCATCCCCAAAGATCTCCCTGAGACCCTGAA CGAACTTCACCTGGACCACAACAAAATCCAGGCTATTGAGTTGGAGGACCTACTTCGATACTCCAAGCTG TACAGGTTGGGCTTAGGTCACAATCAGATTCGGATGATTGAGAATGGGAGCCTGAGTTTTCTGCCTACCC TGAGGGAACTTCACTTGGACAACAACAAGCTGTCCCGGGTGCCTGCTGGCCTCCCAGATCTCAAGCTCCT CCAGGTTGTCTATCTGCACTCCAACAACATCACCAAGGTGGGCATCAATGACTTCTGTCCTATGGGCTTC GGAGTCAAGAGGGCCTACTATAATGGCATCAGCCTCTTCAACAACCCTGTGCCCTACTGGGAAGTGCAGC CTGCCACCTTCCGCTGCGTTACTGACCGCCTGGCCATCCAATTTGGAAATTATAAGAAGTAGAGGCAGTG GTTGCCACCATGGTGGCCTTGGTGAGAGTCTCTGAGGAACATAGCCAGATGAAGAAGCAACACCTTTGTT CCCCAATATTAACTCACTGCCCCACCACAGCTTCCCCCTGACTCCTAAGCATGCATATATGCACATGGCC TGGCCCTCTCACCCATTCCCCTCAACCTTTGAAATTTAACATTCATCAACCATGTCCACTCAGAGACTCC CTATAAATCTTTCTTCTTGCTCATCCTGAAACTCAGATGTTTTTGGCAAGAGGGGCTAGGAAAGATGGAT AGAGCACACTGCCACCGCCATTGTTCCATCCAGGCATGTGTTCCTCCTCTTCCTTGCTCATGTCTGACTT CCAGCTCTCCTGGGCTCTGCTTGCTGCCCTTATCCTCTGGTGTTCTCTCTTCAACAAGTTCACTACCTGT CAATCCCAGCTACAACCTGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACCCTGTTATGCTTCCT GACACTTTTCTTCCTTCTGGAGTTATTGACCTGTCCCCTTCCATCTCTGGACCTAGGTCATATTTCTCCA TCTTTGTCTCTTTCTGTATCTCCTTGCCTATATCTCTGTCTGTCTCTATTTCTGTCTCTCTGTCTCTGTG TATCTCTCTATCTTTGTATCTGTCTCTCTCTGACAACACACACACACACACACACACACACACACACACA CACACGGATCATCTGCCCCAGGCTGCTTTCTGCTTCACAGGTCTCTAGCCAGTCCCTCCACAAACAAATA TGGGGCAACTATCTTCCTGATTGCCCTACCCAGAACTTGACCCCCCAACCCTGGAGGAAGCTGGAAGGTG GAGGCCCAGAATCCTGTCCATTTTGTCCAGGAAAGGGTTCATACTCTGCTATCAAGACGAGGATCAAGGA GCTTCCTAGCCCCTGGAGAGGCTCAGCAGGCCATCAGAGCCGCCAGAACCAGTTTGCATTGGCCCCTGCT CTCTCCCCAAGATGGCTAGGTCCCCTCCCTCACCCCTGGGTCCCTGATGTGGTAGGAGGTGATGGTCAGT TGCACCCAGCAAGAGGGAGTGCTGCTTATGAGGTCAGTTGTCTCTCAATTAAAGAAACACTGTGCAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4237268 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4237268 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005068 same experiment
	EMAGE:30616 same embryo
	EMAGE:30594 same embryo
	EMAGE:31899 same embryo
	EMAGE:30424 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source