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EMAGE:31544

Pcp4 ( MGI:97509)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544
euxassay_006129_01 euxassay_006129_02 euxassay_006129_03 euxassay_006129_04 euxassay_006129_05
EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544
euxassay_006129_06 euxassay_006129_07 euxassay_006129_08 euxassay_006129_09 euxassay_006129_10
EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544
euxassay_006129_11 euxassay_006129_12 euxassay_006129_13 euxassay_006129_14 euxassay_006129_15
EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544 EMAGE:31544
euxassay_006129_16 euxassay_006129_17 euxassay_006129_18 euxassay_006129_19 euxassay_006129_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 04 10 11
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 03 04 05 14 15 16
rest of cerebellum mantle layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 09 10 11 12 13 14 16 17 moderate expression: see section 01 07 15
rectum
weak weak
regionalweak expression: see section 08 09 10 11
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 13 14
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 05 10 weak expression: see section 07 09
esophagus
moderate moderate
regionalmoderate expression: see section 06 07
vagus x ganglion
strong strong
homogeneousstrong expression: see section 03 13 14
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 10 17 18 19 moderate expression: see section 07 08 09 11 12 13 14 15 16 20
trigeminal v ganglion
moderate moderate
spottedmoderate expression: see section 02 03 04 05 06 14 15 16 17 18
tibia
moderate moderate
regionalmoderate expression: see section 01
femur
moderate moderate
regionalmoderate expression: see section 02
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 13 14 weak expression: see section 06 07 08 15 16
cervical ganglion
moderate moderate
regionalmoderate expression: see section 03 04 12
naris
moderate moderate
regionalmoderate expression: see section 13
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain mantle layer
strong strong
gradedstrong expression: see section 09 moderate expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 15 16
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12
mandible
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 15 16 17 18 19
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 07
stomach
weak weak
regionalweak expression: see section 05
kidney calyx
moderate moderate
regionalmoderate expression: see section 02 03 04 05 11 12 13
fibula
moderate moderate
regionalmoderate expression: see section 01
rib
moderate moderate
regionalmoderate expression: see section 19 20 weak expression: see section 01 02 03 04 05 06 07 10 11 12 14 15 16 17 18
pons ventricular layer
strong strong
regionalstrong expression: see section 09 moderate expression: see section 08
pons mantle layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 08 12 13 14 15 not examined expression: see section 09
midgut
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 14 15
dorsal root ganglion
moderate moderate
spottedmoderate expression: see section 02 03 05 06 07 08 09 10 11 12
hypothalamus
strong strong
regionalstrong expression: see section 06 07 08 11 12 13 14 moderate expression: see section 05 09 15
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 04 05 11 12 moderate expression: see section 03 06 07 08 09 10 13 14
maxilla
moderate moderate
regionalmoderate expression: see section 03 04 05 06 15 16 17 19 20
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 10 12 13 14 moderate expression: see section 05 06 07 08 09 11 15 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9661
Entity Detected:Pcp4, ( MGI:97509)
Sequence:sense strand is shown

>T9661
GCGCTCTCGGATGTCTGCAGGCGTTCAGACAGGTGAATGCCTCTCATTGGTTGGCGGGAGGCATGATGCC
ACCCAGCAGCTCTTTTTAAGAGAAAAGCCAGGACCAGGGAAGCAGCCACCCCTGAGCATTGCCTTCTGAG
CTGTTCTGTGGGACCTAGCTGCGGAGTCAGGCCAACATGAGTGAGAGACAAAGTGCCGGAGCGACCAACG
GAAAAGACAAGACGTCAGGAGATAATGATGGGCAGAAGAAAGTCCAAGAAGAATTTGATATCGACATGGA
TGCACCAGAGACAGAGCGTGCAGCTGTGGCCATTCAGTCTCAGTTCAGAAAATTCCAGAAGAAAAAGGCA
GGATCACAGTCCTAGTGGTGAAGCTGCTTCCTGGTCCACCTGAAGACACCAAGTTCAACCACCATCCATC
AAGAAATGAAAAGAACAATACCCTAGAGAGAAGTCATCCTCACTCAAGACACACCCTGCTACAAACCTGA
AATGCATGAAGAGAAACCCATAGTATTTATGCCCCCTATAGGCAGGTATCCACAGTAAAATTGTGAGTAG
CTTAATCTGTTTATCTCCATTACAATTCCTCTGCAACTATTTTCCTTGATGTTGTAATAAAAAGGAGGTA
AGATGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6593949 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6593949 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006129 same experiment
 EMAGE:29607 same embryo
 EMAGE:29588 same embryo
 EMAGE:29612 same embryo
 EMAGE:29603 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS