EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31555

Ace2 ( MGI:1917258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005038_01	euxassay_005038_02	euxassay_005038_03	euxassay_005038_04	euxassay_005038_05

euxassay_005038_06	euxassay_005038_07	euxassay_005038_08	euxassay_005038_09	euxassay_005038_10

euxassay_005038_11	euxassay_005038_12	euxassay_005038_13	euxassay_005038_14	euxassay_005038_15

euxassay_005038_16	euxassay_005038_17	euxassay_005038_18	euxassay_005038_19	euxassay_005038_20

euxassay_005038_21	euxassay_005038_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 07 08 09 10 11

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 14 15

renal medullary interstitium

strong

regional

strong expression: see section 08 09 10 14 15 16 17 18 19 20 21 moderate expression: see section 07

adrenal cortex

strong

regional

strong expression: see section 08 09 moderate expression: see section 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7474
Entity Detected:	Ace2, ( MGI:1917258)
Sequence:	sense strand is shown >T7474 CATATTGGTCCAGCAGCTTGTTTACTGTTCTCTTCTGTTTCTTCTTCTGCTTTTTTTTTCTTCTCTTCTC AGTGCCCAACCCAAGTTCAAAGGCTGATGAGAGAGAAAAACTCATGAAGAGATTTTACTCTAGGGAAAGT TGCTCAGTGGATGGGATCTTGGCGCACGGGGAAAGATGTCCAGCTCCTCCTGGCTCCTTCTCAGCCTTGT TGCTGTTACTACTGCTCAGTCCCTCACCGAGGAAAATGCCAAGACATTTTTAAACAACTTTAATCAGGAA GCTGAAGACCTGTCTTATCAAAGTTCACTTGCTTCTTGGAATTATAATACTAACATTACTGAAGAAAATG CCCAAAAGATGAGTGAGGCTGCAGCCAAATGGTCTGCCTTTTATGAAGAACAGTCTAAGACTGCCCAAAG TTTCTCACTACAAGAAATCCAGACTCCGATCATCAAGCGTCAACTACAGGCCCTTCAGCAAAGTGGGTCT TCAGCACTCTCAGCAGACAAGAACAAACAGTTGAACACAATTCTGAACACCATGAGCACCATTTACAGTA CTGGAAAAGTTTGCAACCCAAAGAACCCACAAGAATGCTTATTACTTGAGCCAGGATTGGATGAAATAAT GGCGACAAGCACAGACTACAACTCTAGGCTCTGGGCATGGGAGGGCTGGAGGGCTGAGGTTGGCAAGCAG CTGAGGCCGTTGTATGAAGAGTATGTGGTCCTGAAAAACGAGATGGCAAGAGCAAACAATTATAACGACT ATGGGGATTATTGGAGAGGGGACTATGAAGCAGAGGGAGCAGATGGCTACAACTATAACCGTAACCAGTT GATTGAAGATGTAGAACGTACCTTCGCAGAGATCAAGCCATTGTATGAGCATCTTCATGCCTATGTGAGG AGGAAGTTGATGGATACCTACCCTTCCTACATCAGCCCCACTGGATGCCTCCCTGCCCATTTGCTTGGTG ATATGTGGGGTAGATTTTGGACAAATCTGTACCCTTTGACTGTTCCCTTTGCACAGAAACCAAACATAGA TGTTACTGATGCAATGATGAATCAGGGCTGGGATGCAGAAAGGATATTTCAAGAGGCAGAGAAATTCTTT GTTTCTGTTGGCCTTCCTCATATGACTCAAGGATTCTGGGCAAACTCTATGCTGACTGAGCCAGCAGATG GCCGGAAAGTTGTCTGCCACCCCACAGCTTGGGATCTGGGACACGGAGACTTCAGAATCAAGATGTGTAC AAAGGTCACAATGGACAACTTCTTGACAGCCCATCACGAGATGGGACACATCCAATATGACATGGCATAT GCCAGGCAACCTTTCCTGCTAAGAAACGGAGCCAATGAAGGGTTCCATGAAGCTGTTGGAGAAATCATGT CACTTTCTGCAGCTACCCCCAAGCATCTGAAATCCATTGGTCTTCTGCCATCCGATTTTCAAGAAGATAG CGAAACAGAGATAAACTTCCTACTGAAACAGGCATTGACAATTGTTGGAACACTACCGTTTACTTACATG TTAGAGAAGTGGAGGTGGATGGTCTTTCGGGGTGAAATTCCCAAAGAGCAGTGGATGAAAAAGTGGTGGG AGATGAAGCGGGAGATCGTTGGTGTGGTGGAGCCTCTGCCTCATGATGAAACATACTGTGACCCTGCATC TCTGTTCCATGTTTCTAATGATTACTCATTCATTCGATATTACACAAGGACCATTTACCAATTCCAGTTT CAAGAAGCTCTTTGTCAAGCAGCTAAGTATAATGGTTCTCTGCACAAATGTGACATCTCAAATTCCACTG AAGCTGGGCAGAAGTTGCTCAAGATGCTGAGTCTTGGAAATTCAGAGCCCTGGACCAAAGCCTTGGAAAA TGTGGTAGGAGCAAGGAATATGGATGTAAAACCACTGCTCAATTACTTCCAACCGTTGTTTGACTGGCTG AAAGAGCAGAACAGAAATTCTTTTGTGGGGTGGAACACTGAATGGAGCCCATATGCCGACCAAAGCATTA AAGTGAGGATAAGCCTAAAATCAGCTCTTGGAGCTAATGCATATGAATGGACCAACAACGAAATGTTCCT GTTCCGATCATCTGTTGCATATGCCATGAGAAAGTATTTTTCAATAATCAAAAACCAGACAGTTCCTTTT CTAGAGGAAGATGTACGAGTGAGTGATTTGAAACCAAGAGTCTCCTTCTACTTCTTTGTCACCTCACCCC AAAATGTGTCTGATGTCATTCCTAGAAGTGAAGTTGAAGATGCCATCAGGATGTCTCGGGGCCGCATCAA TGATGTCTTTGGCCTGAATGATAACAGCCTGGAGTTTCTGGGGATTCACCCAACACTTGAGCCACCTTAC CAGCCTCCTGTCACCATATGGCTGATTATTTTTGGTGTTGTGATGGCACTGGTAGTGGTTGGCATCATCA TCCTGATTGTCACTGGCATCAAAGGTCGAAAGAAGAAAAATGAAACAAAAAGAGAAGAGAACCCTTATGA CTCGATGGACATTGGAAAAGGAGAAAGCAATGCAGGATTCCAAAACAGTGATGATGCTCAGACTTCCTTT TAGCAAAGCACTTGTCATCTTCCTGTATGTAAATGCTAACTTCATAGTACACAAAATATGAGAGTATACA CATGTCATTAGCTATCAAAACTATGATCTGTTCAGTAAACGTTGTCCAAAGAGCATCAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236529 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236529 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005038 same experiment
	EMAGE:31859 same embryo
	EMAGE:31505 same embryo
	EMAGE:29948 same embryo
	EMAGE:30163 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source