EMAGE mouse gene expression spatial database

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EMAGE:31568

Aire autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) ( MGI:1338803)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

outer ear

moderate

regional

moderate expression: see section 01 02 03 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55002
Entity Detected:	Aire, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) ( MGI:1338803)
Sequence:	sense strand is shown >T55002 GGAATTCAGACCATGGCAGCTTCTGTCCAGAGAGCTGTGACCGTGGCCTCTGGGGATGTTCCAGGAACCC GAGGGGCCGTGGAAGGGATCCTTATCCAGCAGGTGTTTGAGTCAGGAAGATCCAAGAAGTGCATTCAGGT TGGGGGAGAGTTTTATACACCCAACAAGTTCGAAGACCCCAGTGGCAATTTGAAGAACAAGGCCCGGAGT GGTAGCAGCCTAAAGCCAGTGGTCCGAGCCAAGGGAGCCCAGGTCACTATACCTGGTAGAGATGAGCAGA AAGTGGGCCAGCAGTGTGGGGTTCCTCCCCTTCCATCCCTCCCCAGTGAGCCCCAGGTTAACCAGAAGAA CGAGGATGAGTGTGCCGTGTGCCACGACGGAGGTGAGCTCATCTGTTGTGACGGCTGTCCCCGGGCCTTC CACCTGGCTTGCCTGTCCCCACCTCTGCAGGAGATCCCCAGTGGCCTCTGGAGATGCTCCTGCTGCCTCC AGGGCAGAGTCCAACAGAACCTGTCCCAGCCTGAGGTGTCCAGGCCCCCGGAGCTACCTGCAGAGACCCC GATCCTCGTGGGACTGAGGTCAGCTTCAGAGAAAACCAGGGGCCCATCCAGGGAGCTCAAAGCCAGCTCT GATGCTGCTGTCACATATGTGAACCTGCTGGCCCCGCACCCTGCAGCTCCTCTGCTGGAGCCTTCAGCAC TGTGCCCTCTACTGAGTGCTGGGAATGAGGGGCGGCCAGGTCCAGCACCAAGCGCGCGATGCAGTGTGTG TGGCGATGGCACCGAGGTGTTGCGGTGTGCACACTGTGCCGCTGCCTTCCACTGGCGCTGCCACTTCCCG ACGGCCGCCGCCCGGCCGGGGACCAATCTCCGCTGCAAATCCTGCTCTGCAGACTCGACTCCCACGCCAG GCACACCGGGCGAAGCTGTACCCACCTCTGGGCCCCGTCCAGCACCTGGGCTTGCCAAGGTAGGGGACGA CTCTGCTAGTCACGACCCTGTTCTACATAGGGACGACCTGGAGTCCCTCCTCAATGAGCACTCATTTGAC GGCATCCTGCAGTGGGCCATCCAGAGCATGTCACGCCCGCTGGCCGAGACACCACCCTTCTCTTCCTGAT GACAGGTGGCCCAGGAAGGGGTGGGCAGCACAGCATTGGCTCCCTCCCCACCCAGCCCCATCGGATGAGG CACTCTGTTCTGAGAGGCCTGGGCTGATTAGGACCAAGAGCTGGCAGGTTCTGGCCTGCTGGACTCAGCT TGCAGATGGCCCTGATCTTTGTAGAGATGCAAGGCCACCCCATATCCTGGAATTAAAGTCACTTCTATGT ACAAAAAAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_019679 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source