EMAGE mouse gene expression spatial database

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EMAGE:31581

Ccdc67 ( MGI:2443026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008557_01	euxassay_008557_02	euxassay_008557_03	euxassay_008557_04	euxassay_008557_05

euxassay_008557_06	euxassay_008557_07	euxassay_008557_08	euxassay_008557_09	euxassay_008557_10

euxassay_008557_11	euxassay_008557_12	euxassay_008557_13	euxassay_008557_14	euxassay_008557_15

euxassay_008557_16	euxassay_008557_17	euxassay_008557_18	euxassay_008557_19	euxassay_008557_20

euxassay_008557_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

lateral ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 15 16 moderate expression: see section 12 13 14

4th ventricle

strong

regional

strong expression: see section 03 04 05 06 07 08 09 15 16 17 18 moderate expression: see section 01 02 12 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35687
Entity Detected:	Ccdc67, ( MGI:2443026)
Sequence:	sense strand is shown >T35687 GAAACTAGAGGAGTTCCGAGCAAAGTCGAGGGAGTGGGACAAGCAGGAGGTCCTCTACCAGACCCACCTG GTGTCCCTGGATGCTCAGCAGAAGTTGCTCTCTGAAAAGTGCAGCCAGTTTCAGAAACAGGCACAAAATT ACCAAACTCAGCTAAACGGGAAAAAGCAGTGCGCAGAGGACAGCAGCTCTGAGATCCCTCGCCTGGTGTG TGAGTCGGATCCTGGCTGTGAAGCCACTCAAAGAGATGAGTTCATTATCGAGAAGTTAAAGTCGGCCGTG AGTGAAATCGCGCTGAGCAGGAACAAGTTACAAGATGAAAACCAGAAGCTCCTGCAGGAACTGAAGATGT ACCAGAGGCAGTGCCAGGCCATGGAAGCAGGACTGTCAGAGGTGAAGAGTGAGTTACAGTCGCGAGACGA TCTCCTGAGGATTATAGAAATGGAAAGACTGCACTTGCATAGAGAACTGCTGAGAATGGGTGAAGTGCAG ACTGCTCAAGATAACAGGAAAAGAGTTGAGTCATCATATTCCCCTTCTCCTAAAGAAGCAGAAAGGAAAA GGAAGGAGTTGTTTCCAATGGTCTCAGATCAACCCAATCATGAAAAGGAATTAAGCAAGATGAGAAGCCA GCTCTATCAGGAGGAAGGCCTATGCTCCGAGCAGGAACGGTTGAGGAGTGAGATCTCGGAACTCACCCAG GAACTTCACCAGAAGGAGGTCACTAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 72032. Forward Primer - name:072032_F_cDNA_4933401K09Rik, sequence:GAAACTAGAGGAGTTCCGAGCA; Reverse Primer - name:072032_N_SP6_cDNA_4933401K09Rik, sequence:ATAGTGACCTCCTTCTGGTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008557 same experiment
	EMAGE:31892 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source