EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31594

Gpam glycerol-3-phosphate acyltransferase, mitochondrial ( MGI:109162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005492_01	euxassay_005492_02	euxassay_005492_03	euxassay_005492_04	euxassay_005492_05

euxassay_005492_06	euxassay_005492_07	euxassay_005492_08	euxassay_005492_09	euxassay_005492_10

euxassay_005492_11	euxassay_005492_12	euxassay_005492_13	euxassay_005492_14	euxassay_005492_15

euxassay_005492_16	euxassay_005492_17	euxassay_005492_18	euxassay_005492_19	euxassay_005492_20

euxassay_005492_21	euxassay_005492_22	euxassay_005492_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 13

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

single cell

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 08 09 10 11

pons ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 16 moderate expression: see section 17 18

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4747
Entity Detected:	Gpam, glycerol-3-phosphate acyltransferase, mitochondrial ( MGI:109162)
Sequence:	sense strand is shown >T4747 CCACGCGTCCGAGCGCTGGCAGCTGCGTGCGCCCAGGCGCGAGCTGCCGGGCGGGTGGAGAGGAGCGCGG TGCGCTGTGCGCGGTGCGGGCTGCGGCTGCAGACGCCGCTGGGCCGGAGCGCGGGGTCAGCACATGGTTT GGGACTTGCACGTTCTGCCATGGAGGAGTCTTCAGTGACAGTTGGCACAATAGACGTTTCTTATCTGCCC AGTTCATCGGAATACAGCCTTGGCCGATGTAAACACACCAGTGAGGACTGGGTTGACTGTGGGTTCAAAC CTACCTTCTTCAGATCTGCAACACTGAAATGGAAGGAGAGCCTTATGAGCCGGAAGAGGCCCTTCGTGGG AAGGTGCTGCTATTCCTGCACGCCACAGAGCTGGGAAAGGTTTTTCAACCCCAGTATCCCATCTCTGGGT TTGCGGAATGTTATTTATATCAATGAAACGCACACAAGGCACAGAGGATGGCTGGCGAGACGGCTGTCTT ACATCCTTTTTGTTCAAGAGCGAGACGTCCATAAGGGCATGTTTGCCACCAGTGTTACTGAGAATGTACT GAGCAGCAGCAGAGTCCAAGAGGCAATTGCTGAAGTGGCTGCGGAGTTGAACCCAGATGGATCTGCCCAG CAGCAGTCCAAAGCCATCCAGAAGGTGAAAAGGAAAGCCAGGAAGATCCTCCAGGAGATGGTCGCCACCG TCTCCCCAGGGATGATCAGGCTGACTGGCTGGGTGTTACTAAAGCTCTTCAACAGCTTCTTCTGGAACAT TCAGATTCACAAGGGTCAACTCGAGATGGTCAAGGCTGCAACTGAGACGAACCTGCCGCTCTTGTTTCTG CCGGTGCACAGATCCCACATTGACTACCTGTTGCTCACCTTCATCCTCTTTTGCCACAACATCAAGGCGC CGTACATCGCCTCGGGCAATAATCTCAACATCCCCGTCTTCAGTACCTTGATTCACAAGCTTGGGGGCTT TTTCATAAGACGGAGGCTCGATGAAACCCCAGATGGACGCAAAGACATTCTGTACAGAGCGTTGCTCCAT GGGCATGTAGTTGAACTCCTCCGACAGCAGCAGTTCCTGGAGATCTTCCTGGAAGGCACCCGCTCCCGCA GTGGCAAGACCTCCTGTGCCCGGGCAGGGCTCCTCTCAGTGGTAGTGGATACTCTGTCGTCCAACACCAT CCCCGACATCCTCGTCATACCCGTGGGCATCTCGTATGATCGCATAATCGAAGGTCACTACAATGGCGAA CAGTTGGGAAAGCCCAAGAAGAACGAGAGCCTCTGGAGTGTGGCGAGAGGCGTTATCAGAATGCTGCGGA AAAACTACGGCTACGTCCGAGTGGATTTTGCACAGCCATTTTCCTTGAAGGAATATTTAGAAGGCCAGAG TCAGAAACCTGTATCTGCCCCCCTTTCTCTGGAGCAAGCACTGTTACCAGCGATCCTTCCTTCAAGACCG AATGATGTTGCTGATGAACATCAAGACCTATCCAGTAACGAGTCCAGAAACCCAGCAGACGAAGCCTTCC GACGAAGGCTGATTGCAAACCTGGCTGAGCACATTCTCTTCACCGCCAGCAAGTCCTGCGCTATCATGTC CACCCACATTGTGGCCTGTCTGCTCCTCTACAGACACAGGCAGGGAATCCATCTCTCCACGCTTGTGGAA GACTTCTTTGTGATGAAGGAGGAAGTCCTAGCTCGCGATTTCGACCTAGGCTTCTCCGGGAATTCAGAAG ATGTCGTCATGCATGCTATTCAGCTTCTGGGGAACTGTGTCACAATCACCCACACGAGCAGGAAAGATGA GTTTTTTATTACTCCCAGCACAACTGTCCCGTCAGTCTTTGAACTCAACTTCTACAGCAATGGCGTACTT CATGTGTTCATCATGGAAGCCATCATAGCTTGCAGCATCTATGCAGTCCTGAATAAGAGGTGCTCTGGAG GGTCCGCTGGAGGCCTCGGCAACCTGATCAGCCAGGAGCAGCTGGTGAGGAAGGCCGCCAGCCTGTGCTA CCTTCTCTCTAACGAAGGTACCATTTCTCTGCCCTGCCAGACTTTTTACCAAGTTTGTCATGAGACAGTT GGCAAGTTCATCCAGTATGGCATTCTCACAGTGGCAGAGCAAGATGACCAGGAAGATGTCAGTCCTGGCC TTGCAGAACAGCAGTGGGACAAGAAGCTTCCGGAACTGAACTGGAGAAGTGACGAGGAAGATGAAGACAG TGACTTTGGTGAGGAGCAGCGAGATTGCTATCTCAAGGTGAGCCAGTCCAAGGAGCACCAGCAATTCATC ACCTTTCTTCAGAGGCTTCTAGGTCCCCTGCTAGAAGCCTACAGCTCTGCTGCCATCTTTGTCCACAACT TCAGCGGTCCAGTTCCCGAGTCTGAGTACCTGCAGAAACTGCACAGGTACCTTATCACCAGGACGGAAAG GAACGTTGCCGTGTACGCTGAGAGTGCCACATACTGTCTCGTGAAGAACGCTGTGAAAATGTTTAAGGAC ATCGGGGTTTTCAAAGAGACCAAGCAAAAGCGAGTGTCTGTTCTAGAACTGAGCAGTACTTTCCTACCTC AGTGCAACCGGCAGAAACTCCTAGAGTATATTCTGAGTTTTGTGGTGCTGTAGCGATCTCTCAGCACCTA TCTCGAGCGAGGAGCTGATGAGTCCCTTGCAGCCTCCAGTCTGACCCAGAGTGGCAGGTATCCTGGCATG GCCCAGCCTGCCCTACCCCGAGGAGACTTCCAGGAAGACACGTGCCTTCTGCCCCCGTGGACCTGCGCTC CTCCCAACACAGACGCAGTGGCCTGCGCAGAGCACTCAGGGCCAGTGCCATCTGTGATTCATGATCACTA GGTTATAGGTGAAATCTCAGTAAGTTATTTTGGAGTTTATTAAAGGTTCACATTTTAAGTACAACTTTTC AGGCTAGTTACTGTGATGGACATTGAGGTGTTTGTAGAACTGACTCTTATATAGTAAGTAATAATGTTTC CTTTAAAATAATTGGGCCATCCATTCTCTGTCTCCATTATCGCTGTCAACAGAAGTTATGGTTTGTATGT GAGCCCTTTCCTCTCAACTGCTTTACTAAGGAGCAATGTTGTCCTGCGCCGGCACACTCAAGATGTATCT GAGTAGCTGCCTGAAAGCAGATGACACTGCAGTCAGGAGGTGAGAGGCTGAGGAAAGAGCCAGCATCAGG AGGTGACTCTGGTTGGCAAGGGTGAGCTGTGCTTGGCCAGGAAGGTGGCATTAAAGGCTGTGCTCACGTG ACAAGAGTGCCTTTGAGCTGTAATGACCAGTGTTTAGAGTCCCTGTTGCTGATACCTTTGAGTGATTGAC ACCTGCTGCTTTTGAGGAGCTCGGCTGCAGTGATCACTGGCGTGTGGCACTCTGGCTACAAACTGCTGAC CCAGGGGTTCCAGACACTGCTCACTCACTGGGTGCTGTGCTGTGGCTAGTGTATGGCTTGTACCCAGATG GCGCACTGTGCAGCCTGTGTAGCATGCACAGTGACCCAGCTGAGTGGCAGGTGGTTGGAGGCAAGGACAT TTATGTGGGCCACATACCCAAACTTCCTGCCATGTAAAGGCGAGTGATTGTGAAAGCACCTCTGTTGTAG TGGGTAGGTGCCTTTCTCATGAGCGGGGAGCATAACTGTTAGTTATTTGTGGTGATTATGGCAGATTTCA AGATCACGTGACAATGCAGTCTCTAATTGTTCACTGTCCATATATATGATTTTACGGAATTATGAATTTT TCTGATGAGTGTTGTATAGATGATTTGAATTGATAAAAGTATTAGGGATGAGGTTTGTAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5041120 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5041120 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005492 same experiment
	EMAGE:30528 same embryo
	EMAGE:31648 same embryo
	EMAGE:31603 same embryo
	EMAGE:29399 same embryo
	EMAGE:30478 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source