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EMAGE:31595

BC006965 ( MGI:2384955)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595
euxassay_005257_01 euxassay_005257_02 euxassay_005257_03 euxassay_005257_04 euxassay_005257_05
EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595
euxassay_005257_06 euxassay_005257_07 euxassay_005257_08 euxassay_005257_09 euxassay_005257_10
EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595
euxassay_005257_11 euxassay_005257_12 euxassay_005257_13 euxassay_005257_14 euxassay_005257_15
EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595 EMAGE:31595
euxassay_005257_16 euxassay_005257_17 euxassay_005257_18 euxassay_005257_19 euxassay_005257_20
EMAGE:31595 EMAGE:31595 EMAGE:31595
euxassay_005257_21 euxassay_005257_22 euxassay_005257_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
saccule
strong strong
regionalstrong expression: see section 09 10
inner ear
strong strong
regionalstrong expression: see section 03
utricle
strong strong
regionalstrong expression: see section 02 08 09 10 19 20 21 22
sternum
strong strong
regionalstrong expression: see section 12 13 14
posterior semicircular canal
strong strong
regionalstrong expression: see section 03 04 05 06 07 23
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 16 17
nose
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
cochlea
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 18 19 20 21 22 23
superior semicircular canal
strong strong
regionalstrong expression: see section 03 04 05 06 07 23
pancreas
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
submandibular gland primordium
strong strong
regionalstrong expression: see section 16 17 18 19 20 21
lateral semicircular canal
strong strong
regionalstrong expression: see section 03 04 05 06 07 23
naso-lacrimal duct
strong strong
regionalstrong expression: see section 05
naris
strong strong
regionalstrong expression: see section 11 12 13 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5836
Entity Detected:BC006965, ( MGI:2384955)
Sequence:sense strand is shown

>T5836
CACGCGTCGGCCACTCCACCTCGGGCGCTCTGAAGCCGGCCGCATGGAGACTGGGGGACGGTGATGGCCA
AGGGACAGTGGAAAAATGGCAGGGCAGACCCCCTTGGATTTGCCCACGTGGAGTTTGGATATTTACCTGG
CTTCTTTGTCAAGTCTTGGGCCTGATGCAGGTAAGTGTGGCGGGGCCCTTGGAGATCTGTTGGAGCAGTT
GCAAGGGGCCACTGTAGACCAGCAATCGCCATGGCTGCAGCATTGGTGCCAAGCAAGAAGTCCCATCATG
TTCCACACACCAAGGCTGAGGAGGCATAGAGTTACCTGCTGCAGATGAGTAGAGTAGCAGCCCTCTGTCC
TTCCATGAAAGGAGACTCCATGGGAGACAATGGTACGTTTCTGTGCGGTCACTAGCACCCTCATGACTTG
CTTACTCTTAACACTTACTTTTTTAACAGGTTGCAGACGTATGGCCACCGGTTCATGTAGTCAACAGGTC
AGGGGGGCTGTTGCCACCGAAGGAAGGGGGATAGTGCTGACAAGACTGATGGTAATGATGACATCTAACG
TACATGGAGCAGCTCTATCTGCCAGACACTAAGCTCACACTGCAAGTGCCTTCTCCCAGAAGACTTGATA
CCGGGATATCCTGAGAGCCAAATCAAAAGCTGCTTTCCACCAGGAGCTTAAGGGAGCACTGAAGAAGACC
AAAGGAACTGTGACTCCCTAAGCTCAGGGAATAGTCAATAAATCTCTTTGCTGGCCCGCTGGCATAGGCT
TGTAGTCTCAGCGACTTATGAGTTGAGGCAGAAGGATAAGTAGTTCAAGGCCAGCCTTGTCAACTTACCA
AAACTGTCTCAAATTTTTGAAAAAGAAAAGCAGAGTAGGGAATATCAGGCAAGGATAGAATGCTTACCCA
GCATGCACCAGGTCACCGAGTCAATCCCTAGAACTGAAGAGAAAAGTGATCTCCTCTTTAAAAGGAACTA
TTTTCTGACTATCCTGGAAATGGATGGTTCTAGAAGAGTTTCATACTCAACCCTGAGACTTGTGGGAAAT
CTCAGATTTGGAGTCTTCGTAGCTAACCACTTCAGGTATAGTGTCCCCAACTGGGATGACAGATAGGAAC
CTCACAGTGTCCCCAGTCTCATGTTCCTTTTGCCTTAGGAAATCCTTGGGGTCTAAAACTGCACCAAGTA
TAAGAGATAACTCCCCTTACCTCCTTGGAGATGAGGATTGGTACCATTATGTACAGACTTTAGGCAGGGT
GCTCAAGTCCTCCCTCACCCATCTCTTCTTGTCTAACCTAAGGGATAAAGAGCTTGCTTTCAAATGTTAT
TAACACTGGGACAATGGGCAAGAGAACACTAATGTTTCGTGATGCATGAAATAAAAAGAAATTTACATTT
AAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3153901 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3153901 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005257 same experiment
 EMAGE:30142 same embryo
 EMAGE:31240 same embryo
 EMAGE:30234 same embryo
 EMAGE:30211 same embryo
 EMAGE:30226 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS