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EMAGE:31597

Bambi BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) ( MGI:1915260)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597
euxassay_003079_01 euxassay_003079_02 euxassay_003079_03 euxassay_003079_04 euxassay_003079_05
EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597
euxassay_003079_06 euxassay_003079_07 euxassay_003079_08 euxassay_003079_09 euxassay_003079_10
EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597
euxassay_003079_11 euxassay_003079_12 euxassay_003079_13 euxassay_003079_14 euxassay_003079_15
EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597
euxassay_003079_16 euxassay_003079_17 euxassay_003079_18 euxassay_003079_19 euxassay_003079_20
EMAGE:31597 EMAGE:31597 EMAGE:31597 EMAGE:31597
euxassay_003079_21 euxassay_003079_22 euxassay_003079_23 euxassay_003079_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 06 07 08 09 10 18 20
clavicle
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 18 19 20
rib
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 21 22 23 24
male reproductive system
moderate moderate
regionalmoderate expression: see section 13
foot mesenchyme
strong strong
regionalstrong expression: see section 03 04
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 08 09 20
viscerocranium
moderate moderate
regionalmoderate expression: see section 09 10 11 19 20 weak expression: see section 08
bladder
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
meckel's cartilage
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24 moderate expression: see section 03
lower jaw molar
strong strong
regionalstrong expression: see section 06 07 08 09 19 20 21 22
upper jaw molar
strong strong
regionalstrong expression: see section 06 07 08 09 19 20 21 22
basisphenoid bone
strong strong
regionalstrong expression: see section 02 05 06 moderate expression: see section 22
hand mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23
femur
strong strong
regionalstrong expression: see section 05 06 moderate expression: see section 22 23 24
vibrissa
strong strong
regionalstrong expression: see section 06 22 23
upper lip
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 08
foot skin
strong strong
regionalstrong expression: see section 05 06 22 23 24
lower jaw incisor
strong strong
regionalstrong expression: see section 11 12 13 16 17
upper jaw incisor
strong strong
regionalstrong expression: see section 11 12 16 17
glans of male genital tubercle
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17
exoccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 23 24 moderate expression: see section 22 weak expression: see section 07 08
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 23 24 moderate expression: see section 09 14 19 20 21 22 weak expression: see section 07 08
lower lip
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4648
Entity Detected:Bambi, BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) ( MGI:1915260)
Sequence:sense strand is shown

>T4648
GTGGTTACGTCCAGTTCCCGTCCGCTGTTCGTGGCTTGTGCGCGGGCTGGCGGGGCGAGAGGACGACCGC
CGAGGAGCGCGCGGGTCCGGGAACGTGTCCTCGGTAGTCTTAGTCCCGCTCAATGCTTCGGCCCCCGCCC
GGACACTGACGGCGCCCGGGCTCGTGCTCGGCGCGCCCCGGGGGTCGTAGTGCAGCCGCGCCTGGCTCCC
GGAGCGACGGGAGCGCGAGGCGTCAATGGATCGCCACTCCAGCTACTTCTTCATCTGGCTGCAGCTGGAG
CTCTGCGCCATGGCTGTGCTGCTCACCAAAGGAGAGATCAGATGCTACTGTGATGCTGCTCACTGTGTGG
CAACAGGTTACATGTGTAAGTCTGAGCTTAGTGCCTGCTTCTCCAGACTTCTCGATCCTCAGAACACCAA
TTCTCCACTCACCCACGGCTGCCTGGACTCACTTGCAAGCACGGCAGACATCTGCCGAGCCAAACAGGCC
CAAAACCACTCTGGCCCTGCCATGCCCACTTTGGAATGCTGTCACGAAGACATGTGCAATTATCGAGGAC
TGCATGACGTCCTCTCTCCTTCCAAGAGCGAAGCCTCAGGACAAGGAAACCGGTATCAGCATGACAGCAG
CAGAAACCTCATCACTAAGATGCAGGAGCTGACTTCCTCCAAAGAACTGTGGTTCCGCGCAGCTGTGATA
GCGGTTCCCATTGCTGGCGGACTGATCTTGGTGCTGCTCATTATGTTGGCCTTGCGAATGCTTCGAAGTG
AGAACAAGAGGCTGCAGGATGAGCGGCAGCAGATGCTGTCCCGTCTGCACTACAGCTTTCACGGACACCA
TTCCAAGAAGGGGCAAGTTGCAAAGTTGGACTTGGAGTGCATGGTGCCTGTCAGTGGGCAGGAGAACTGC
TGTCTGACCTGTGACAAAATGCGGCAAGCAGAGCTCAGTAATGAGAAGATCCTCTCCCTTGTGCACTGGG
GCATGTACAGTGGTCATGGAAAGCTGGAATTCATATGACAGTCATGTCTGAACTATAAATGGAACCCTTG
AAGGCCTTCTGAGTTCTGCTGACAGGAGCACTTTTATCTGGAGACAACTAACTCGCTTAGTCATCTTGGA
GAAACAAAATGACCTCTGAAAAGAGAATCTTGGGTATTTCTTCTGAAGGATTATTTGCACAGATTCTACA
GTCAAATGGATTAATTTGCTTTAAAGTTATAAAAAGCAAAGAGAGGACTCTGCACACTTTCCCAGGGTTC
TTTGTGTCCTAGGGAGCTGCAGCTGACTGAAGTAAAAACACTGTGCTCTCTGTAAGCTCCTACATCCTCA
TTTATTGTAAAGATTTTTTAAAATATATATATTTTTGTCCGAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5009872 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5009872 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003079 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS