EMAGE mouse gene expression spatial database

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EMAGE:31603

Scnn1g ( MGI:104695)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005496_01	euxassay_005496_02	euxassay_005496_03	euxassay_005496_04	euxassay_005496_05

euxassay_005496_06	euxassay_005496_07	euxassay_005496_08	euxassay_005496_09	euxassay_005496_10

euxassay_005496_11	euxassay_005496_12	euxassay_005496_13	euxassay_005496_14	euxassay_005496_15

euxassay_005496_16	euxassay_005496_17	euxassay_005496_18	euxassay_005496_19	euxassay_005496_20

euxassay_005496_21	euxassay_005496_22	euxassay_005496_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 19 20 21 moderate expression: see section 22

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 21 22 23

right lung

moderate

regional

moderate expression: see section 12 13 14 15 16 weak expression: see section 10 11 17 18 19 20

kidney calyx

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17

left lung

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 not examined expression: see section 18

bladder

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4761
Entity Detected:	Scnn1g, ( MGI:104695)
Sequence:	sense strand is shown >T4761 CCACGCGTCCGGGCCAGAAGAAGAGGAGCGTCTCCAGACTGTACACAGGCCAGCCGTGACCCTTCAGTTC AGAAAGAACTCTGCAGGCTGCAAAGTCCTGTCCTTACCATGGCGCCTGGAGAGAAGATCAAAGCCAAAAT CAAAAAGAATCTGCCAGTTCGAGGCCCCCAGGCACCGACCATTAAGGACCTGATGCATTGGTACTGCCTG AACACCAACACCCACGGCTGCCGGCGCATCGTGGTGTCCCGAGGCCGCCTTCGGCGCCTGTTGTGGATTG CGTTCACGCTGACAGCAGTGGCCCTCATTATCTGGCAGTGCGCCCTCCTCGTCTTCTCTTTCTACACCGT CTCTGTCTCCATCAAAGTCCACTTCCAGAAACTGGATTTCCCCGCTGTCACTATCTGCAATATCAACCCC TACAAGTACAGTGCTGTGAGTGACCTCCTGACTGACTTGGACAGTGAGACAAAACAGGCCTTGCTGTCCT TGTATGGGGTCAAAGATGTTCTAGACAGTACACCTCGGAAACGCCGGGAAGCAGGATCCATGCGGTCCAC GTGGGAAGGCACACCACCCAGATTTCTCAACCTGATCCCATTGTTGGTCTTCAATGAGAACGAGAAGGGA AAGGCCAGGGACTTCTTCACTGGTCGGAAGCGGAAAATCAGTGGGAAAATCATACACAAGGCTTCTAATG TCATGCACGTTCATGAGTCGAAGAAACTGGTGGGATTTCAGTTGTGCTCAAATGACACCTCTGACTGTGC CACCTACACCTTCAGCTCAGGAATCAATGCCATCCAGGAGTGGTACAAACTTCACTACATGAATATCATG GCACAGGTGCCTCTAGAGAAGAAAATCAACATGAGCTATTCTGCCGAAGAACTGCTGGTGACCTGCTTCT TCGATGGGATGTCCTGTGATGCCAGGAACTTCACTCTTTTCCACCATCCAATGTATGGGAACTGCTACAC GTTCAACAACAGAGAAAACGCCACCATCCTCAGCACCTCCATGGGAGGCAGTGAGTATGGTCTGCAAGTC ATCTTATACATAAACGAAGATGAGTACAACCCCTTCCTGGTGTCATCCACTGGAGCCAAGGTGCTTGTCC ATCAGCAGAATGAATACCCTTTCATCGAAGACGTGGGGACCGAGATCGAGACAGCAATGTCCACTTCCAT AGGAATGCACCTGACAGAATCCTTCAAGCTGAGCGAACCTTACAGCCAGTGCACAGAGGACGGCAGCGAT GTGCCCGTCACAAACATCTACAATGCTGCCTATTCCCTCCAGATCTGCCTTTACTCATGCTTCCAAACGA AGATGGTGGAAAAATGTGGCTGTGCCCAGTACAGTCAGCCTCTACCTCCAGCAGCCAATTACTGCAACTA CCAGCAACACCCCAACTGGATGTATTGCTACTACCAGCTGTACCAGGCCTTTGTCCGGGAAGAACTGGGC TGCCAATCAGTGTGCAAGCAATCCTGCAGCTTTAAGGAATGGACACTGACCACCAGCTTGGCACAGTGGC CATCTGAGGCTTCTGAGAAATGGTTGCTGAATGTTCTCACCTGGGACCAAAGCCAGCAAATAAACAAAAA GCTCAACAAGACTGACCTGGCCAAGCTCTTGATATTCTACAAAGACCTGAACCAAAGATCCATCATGGAG AGCCCAGCCAACAGTATTGAGATGCTCCTGTCTAACTTTGGTGGACAGCTCGGCCTGTGGATGAGCTGCT CGGTCGTCTGTGTCATCGAAATCATCGAAGTCTTCTTCATTGATTTCTTCTCTATTATTGCCCGCCGCCA GTGGCAGAAAGCCAAGGATTGGTGGGCCCGTAGGCGGACACCACCCTCCACTGAGACTCCCTCCAGCCAG CAGGGTCAGGATAATCCAGCCCTGGATACGGACGATGATCTGCCCACTTTTACCTCTGCTATGCGCCTGC CTCCAGCCCCGGAGGCTCCGGTGCCTGGCACACCACCCCCCAGATACAATACCTTGCGCTTGGACAGTGC CTTTTCCTCCCAGCTCACAGACACTCAGTTGACCAATGAGTTCTAAGATAGGATTGGAAAACAGTCATGG TTTGAGGAGACTCTCTTCCTGACACAAATGGTCCTCCTATCCTCGTTCTGGCTTTTGATACATGTCTAAC AGCCTGCTGTGAACAGGATAGGGCATGGAAATCCACAGAAGGACCTGATGGGCCCTGCCCCAACACTCAT AACTTCCAGCATGAGCTATATCCTGCAACCTGGACTCAGCACTTATAAGCTAAAGAGCCAAAGGTAATGA ATGACCTGGGCCAACAGTGAGCTCTTCCTGGGGTCCTGAAAGAGAATGGTGTTTCAGAGCCCCAAGCTCA GGAGTTTGCCCACAATAACCTGGCCCAGCTTGTACCTCAAGCACATGATCTTGGGTAGCAAGGCTGGCCA GCTATTGCCTGATGGGTGTGACAGAAGAAATTAGCACCATCCTTGGATCCCAGCAGTTGGTTGCTGACCT CAGTCATTGACACTGGGACAAGGTGTCCTCTGTGTCCTCAGACAGACAGTTGGCCCAGAGCCTGGGGATC GTGTTTGGAGAGGGCACAGTAGAGAGAGGACAAGGGGTGTGGTAAGATGTCCCCACTCTGAAGCAGGGTG GGGCAGTAGAGAAGGGCCAGTTAAAGGGCATAGCTGGACACTCCTGCCAAATGGTACTCCTATCCTAATC TCAGCTCGAGTGGTATGCTGCGAGCTGTACTAAGCACTTACTCTAGGGCATAACAAACACCAGCCTACAG GCCCAATCCTTTATTTTGTCTTTGAACTGAGAGTATGTGTATGTGTGAGAATGTGTGTATGAATGTGTAT ATATGTATGTGTTTATAATTTTTATATTGCTAATATATACATATATATATATCAAAAAGATAATATTTTG TGACTTGTGAAAATTATAAGATTCAAATGCTAGTGTTTATAAATAAAGTTTTATTGATAATCAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5042876 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5042876 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005496 same experiment
	EMAGE:30528 same embryo
	EMAGE:31648 same embryo
	EMAGE:31594 same embryo
	EMAGE:29399 same embryo
	EMAGE:30478 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source