EMAGE mouse gene expression spatial database

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EMAGE:31604

Fam26e family with sequence similarity 26, member E ( MGI:2143897)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003077_11	euxassay_003077_12	euxassay_003077_13	euxassay_003077_14	euxassay_003077_15

euxassay_003077_16	euxassay_003077_17	euxassay_003077_18	euxassay_003077_19	euxassay_003077_20

euxassay_003077_21	euxassay_003077_22	euxassay_003077_23	euxassay_003077_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

moderate

regional

moderate expression: see section 10 18

thymus primordium

strong

regional

strong expression: see section 13 17 moderate expression: see section 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4703
Entity Detected:	Fam26e, family with sequence similarity 26, member E ( MGI:2143897)
Sequence:	sense strand is shown >T4703 AGGCAAACGTCTTTCCCTCTTCGCGTGTGTGTGTGTGTGTGTGTGTGTGTCTCCAACATGGATGCCTTTC AGAGCATCTTAAAATTCTTTCTTAACCAAAAAACTGCTATTGGCTACAGCTTCATGGCTTTGCTCACCGT GGGTAGCGAACGTCTCTTCTCGCTGGTGGCATTTAAGTGCCCCTGCAGCGTTGAGAATACTGCCTATGGG CTGGTTTTTCTCTTCGCCCCTGCCTGGGTGTTACTGATCCTTGGCTTCTTTCTGAATAACAAGGCATGGA GACTCTTCACTGGCTGCTGTATGAACCCAAAAAAAATATTTCCCAGGAGACGCTGCTGCCGCTTCTTCTA CGTCCTGGGCCATATCATACTGAGTTCACTGGTGGCTCCGGTGATGTGGCTCTCTGTGGCTTTGCTTAAT GGGACGTTTTATGAATGTGCTATGAGCGGGACAAGAAGCACAAGGCTCCTGGAGATGATTTGCAAGGGCA AGCCCAAAGAGTGCTGGGAAGAACTGCACAAAGTCTCCTGTGGTAAAAGTAGCATGGCCGCTATGGAGAG TGAAGAAGTGAGGCTATCCCTGCAGGCCCAGTCTCAGATCCTAGGATGGTGCCTGATTTGTTCGGCGTCC TTCCTCTCCCTGCTGACCACTTGTTATGCACGCTGCAGATCTAAAGTCAGCTATCTGCAGCTGAGTTTTT GGAAGACATATGCACAAAGGGAGAAGGAGCAACTGGAAAACAAACTCCTGGAATGTGCTAACAAGTTAAG TGAGAGGAACCTCAAATGCTTTTTTGAAAACAAGAAGCCAGATCCCTTTCCCATGCCCTCATTTGGTGCC TGGGAGGCTGCATCCGAGCTACACTCCTTCCATCAAGACCGTGAGCACTACAGCACCCTCCACAAAGTGG TAGATGATGGTATGGAACAAACCCCGCAAGAGGAAGAAACAACAATGATCCTTGTGGGTACTGCCCAGAG TTTGTGAATCATCTACCATTCCTGGCTCAGATATCTAGTGGTACACTCATTCGGAGTTGAGTTTTCTGCT GTTTCCATGACAACCTGAGCCTCTGCATTTTCTCACCATCAGAGCTTCTTTGGAAGATAACACAGTGGGA AACTGTTCTGAAGCCTCGAAGAGCAATGTCTGGATGTGCTCAGACTGATTGATGCTAAGTGATGACAGAC ACTTTGAGCTGGTGGAGGGTTGTGTCCAGGAGGTCCAACAGTTGATCCAAGCTCTTAAGATTTTATAACT CAGCCCTTAGAGTCTGGCAAGATGGTCTGCCACTATTTGTAAGCATCTGTCATGCTATTCTGGGAGAGGT CTGTGCCCAGTGGTATCCACATAGTGGCCATCAGGAACCTTTCAGAAATATATCCAACACCTTTTAAGTT GGAAGGAATTCTGCCATTTAATTAACATTCCTTTCTATAGAATACCTCTATTCCCAAGACTCACTAAATT GAAAGAATATAATATCTAAAAATCAGAGGTTCCTGCATTTACCCCAATTTCTGAAAGTCTGCCTGGAAGA GGGCTGCCACATAACTGCTGTGGGTGATAGGTACTTATACCATCACAGGACAATTTCTACATTCAAAAGA CAATCACATAAGGCATAATACAAGACTGCATTGATATAAAGACAAGTATGTTAATATTGTATATTAAAGT AGTTTTTCACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5031155 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5031155 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003077 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source