EMAGE mouse gene expression spatial database

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EMAGE:31608

Abat ( MGI:2443582)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001907_01	euxassay_001907_02	euxassay_001907_03	euxassay_001907_04	euxassay_001907_05

euxassay_001907_06	euxassay_001907_07	euxassay_001907_08	euxassay_001907_09	euxassay_001907_10

euxassay_001907_11	euxassay_001907_12	euxassay_001907_13	euxassay_001907_14	euxassay_001907_15

euxassay_001907_16	euxassay_001907_17	euxassay_001907_18	euxassay_001907_19	euxassay_001907_20

euxassay_001907_21	euxassay_001907_22	euxassay_001907_23	euxassay_001907_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 06 12

rest of cerebellum mantle layer

strong

regional

strong expression: see section 16

pons ventricular layer

strong

regional

strong expression: see section 05 07 08 09 10 11 12 13 14 15 16

medulla oblongata alar plate mantle layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 15 16 17 18 19

pons mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 11 12 13

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 17 18 19 20 21

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13

cervical ganglion

moderate

regional

moderate expression: see section 06 07 13

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 06 07

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 06 07

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T605
Entity Detected:	Abat, ( MGI:2443582)
Sequence:	sense strand is shown >T605 GTTGGCCTACTGGTATTCATCATATTGGTTTAAGCATGCTGCCTCCTGAGAGCCACAGTGTCATATACAG ATACTTCCGCAGGTCCTTAGAGTTCAAAGGGTTTTAATCCAGGACATAAGCAGAAATCGCTCTCTTTAGT GAAGGGAGCAGCTGATTTTTTCATTTTCTTGCCACAAGGCATGTTGACAGAAGAATAAAATTCTTTGTAG ACAGTCATCTTCAAATGTACTGTCCGCCCCTTGCCTGGAGACAGTTCTTCCCATTTTAAACAAACAAACA AAATTTAAGGCGTTTATCCACAAGTTCTTGACTCGGGTACAGTGCGTTCCTGAGACACCCTCTTTGGGAG CACAGGCAGTTAGCTGGAAGAACTCCAAGGAAAAGAATTTTCCACAAAACTCAAGAATGTCGAGACTTAA CGAAGGCTGATGGAAACCCAGGAAGCCCAGTGGGCAGCTTCTGCCTAGGGCAGAGCCCACAAACCAGTGC GTCTGTGCAGAACGTCTGGATGGACACCCATC
Notes:	The probe template was PCR amplified from IMAGE:1885823 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885823 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001907 same experiment
	EMAGE:31709 same embryo
	EMAGE:30441 same embryo
	EMAGE:30452 same embryo
	EMAGE:30440 same embryo
	EMAGE:30433 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source