EMAGE mouse gene expression spatial database

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EMAGE:31609

Epas1 endothelial PAS domain protein 1 ( MGI:109169)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003082_11	euxassay_003082_12	euxassay_003082_13	euxassay_003082_14	euxassay_003082_15

euxassay_003082_16	euxassay_003082_17	euxassay_003082_18	euxassay_003082_19	euxassay_003082_20

euxassay_003082_21	euxassay_003082_22	euxassay_003082_23	euxassay_003082_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4706
Entity Detected:	Epas1, endothelial PAS domain protein 1 ( MGI:109169)
Sequence:	sense strand is shown >T4706 CTCGGACCTGCGAGCACTAAAGACCTTTCACACCTGCCCGGGCGACAGAGAGCTGCGGAGGGCCACAGCA AAGAGAGCGGCTGCAGCCCCTACGGGGTTAAGGAACCCAGGTGCTCCGGGTCTCGGAGGGCCACGGCGAC AATGACAGCTGACAAGGAGAAAAAAAGGAGCAGCTCAGAGCTGAGGAAGGAGAAATCCCGTGATGCCGCG AGGTGCCGGCGCAGCAAGGAGACGGAGGTCTTCTATGAGTTGGCTCATGAGTTGCCCCTGCCTCACAGTG TGAGCTCCCACCTGGACAAAGCCTCCATCATGCGCCTGGCCATCAGCTTCCTTCGGACACATAAGCTCCT GTCCTCAGTCTGCTCTGAAAATGAATCTGAAGCTGAGGCCGACCAGCAAATGGATAACTTGTACCTGAAA GCCTTGGAGGGTTTCATTGCTGTGGTGACCCAAGACGGTGACATGATCTTTCTGTCGGAAAACATCAGCA AGTTCATGGGACTTACCCAGGTAGAACTAACAGGACACAGCATCTTTGACTTCACTCATCCTTGCGACCA TGAGGAGATCCGTGAGAACCTGACTCTCAAAAACGGCTCTGGTTTTGGGAAGAAGAGCAAAGACGTGTCC ACCGAGCGTGACTTCTTCATGAGGATGAAGTGCACAGTCACCAACAGAGGCCGGACTGTCAACCTCAAGT CGGCCACCTGGAAGGTCCTGCACTGCACCGGGCAAGTGAGAGTCTACAACAACTGCCCCCCTCACAGTAG CCTCTGTGGCTCCAAGGAGCCCCTGCTGTCCTGCCTTATCATCATGTGTGAGCCAATCCAGCACCCATCC CACATGGACATCCCCCTGGACAGCAAGACTTTCCTGAGCCGCCACAGCATGGACATGAAGTTCACCTACT GTGACGACAGAATCTTGGAACTGATTGGTTACCACCCCGAGGAGCTACTTGGACGCTCTGCCTATGAGTT CTACCATGCCCTGGATTCGGAGAACATGACCAAAAGTCACCAGAACTTGTGCACCAAGGGGCAGGTGGTA TCTGGCCAGTACCGGATGCTAGCCAAACACGGAGGATATGTGTGGCTGGAGACCCAGGGGACGGTCATCT ACAACCCCCGCAACCTGCAGCCTCAGTGTATCATGTGTGTCAACTATGTGCTGAGTGAGATCGAGAAGAA CGACGTGGTGTTCTCCATGGACCAGACCGAATCCCTGTTCAAGCCACACCTGATGGCCATGAACAGCATC TTTGACAGCAGTGACGATGTGGCTGTAACTGAGAAGAGCAACTACCTGTTCACCAAACTGAAGGAGGAGC CCGAGGAACTGGCCCAGTTGGCCCCCACCCCAGGAGATGCCATTATTTCTCTCGATTTCGGAAGCCAGAA CTTCGATGAACCCTCAGCCTATGGCAAGGCCATCCTTCCCCCGGGCCAGCCATGGGCCGCGGGGCTGAGG AGCCACAGTGCCCAGAGCGAGTCCGGGAGCCTGCCAGCCTTCACTGTGCCCCAGGCAGGCACCCCAGGGA ACACTACACCCAGTGCTTCAAGCAGCAGTAGCTGCTCCACGCCCAGCAGCCCTGAGGACTACTATTCATC CTTGGAGAATCCCTTGAAGATCGAAGTGATTGAGAAGCTTTTCGCCATGGACACGGAGCCGAGGGACCCG GGCAGTACCCAGACGGACTTCAGTGAACTGGATTTGGAGACCTTGGCACCCTACATCCCTATGGACGGCG AGGACTTCCAGCTGAGCCCCATCTGCCCAGAGGAGCCGCTCATGCCAGAGAGCCCCCAGCCCACCCCCCA GCACTGCTTCAGTACCATGACCAGCATCTTCCAGCCGCTCACCCCGGGGGCCACCCACGGCCCCTTCTTC CTCGATAAGTACCCGCAGCAGTTGGAAAGCAGGAAGACAGAGTCTGAGCACTGGCCCATGTCTTCCATCT TCTTTGATGCTGGGAGCAAAGGGTCCCTGTCTCCATGCTGTGGCCAGGCCAGCACCCCTCTCTCTTCTAT GGGGGGCAGATCCAACACGCAGTGGCCCCCGGATCCACCATTACATTTCGGCCCTACTAAGTGGCCTGTG GGTGATCAGAGTGCTGAATCCCTGGGAGCCCTGCCGGTGGGGTCATCGCAGTTGGAACCTCCGAGCGCCC CGCCTCATGTCTCCATGTTCAAGATGAGGTCTGCAAAGGACTTCGGGGCCCGAGGTCCATACATGATGAG CCCAGCCATGATCGCCCTGTCCAACAAGCTGAAGCTAAAGCGGCAGCTGGAGTATGAGGAGCAAGCCTTC CAAGACACAAGCGGGGGGGACCCTCCAGGCACCAGCAGTTCACACTTGATGTGGAAACGTATGAAGAGCC TCATGGGCGGGACCTGTCCTTTGATGCCTGACAAGACCATCAGTGCGAACATGGCCCCCGATGAATTCAC CCAAAAATCTATGAGAGGCCTGGGCCAGCCACTGAGACACCTGCCACCTCCCCAGCCACCATCTACCAGG AACTCAGGGGAGAACGCCAAGACTGGGTTCCCGCCACAGTGCTATGCCTCCCAGTTCCAGGACTACGGTC CTCCAGGAGCTCAAAAGGTGTCAGGCGTGGCCAGTCGACTGCTGGGGCCATCGTTCGAGCCTTACCTGTT GCCGGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGTGCCTGGAAGCTCCACACTCCTGCAGGGG AGAGACCTTCTCAGAGCTCTGGACCAGGCCACCTGAGCCAGGGCCTCTGGCCGGGCATGCCCCTGCCTGC CCCGCCGTCTTGACCTGCCAGCTTCACTTCCATCTGTGTTGCTATTAGGTATCTCTAACACCAGCACACT TCTTACGAGATGTACTCAACCTGGCCTACTGGCCAGATCACCAAGCAGTGGCCTTTATCTGACATGCTCA CTTTATTATCCATGTTTTAAAAATACATAGTTGTTGTACCTGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5032291 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5032291 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003082 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source