EMAGE mouse gene expression spatial database

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EMAGE:31612

Mmp8 matrix metallopeptidase 8 ( MGI:1202395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003084_01	euxassay_003084_02	euxassay_003084_03	euxassay_003084_04	euxassay_003084_05

euxassay_003084_06	euxassay_003084_07	euxassay_003084_08	euxassay_003084_09	euxassay_003084_10

euxassay_003084_11	euxassay_003084_12	euxassay_003084_13	euxassay_003084_14	euxassay_003084_15

euxassay_003084_16	euxassay_003084_17	euxassay_003084_18	euxassay_003084_19	euxassay_003084_20

euxassay_003084_21	euxassay_003084_22	euxassay_003084_23	euxassay_003084_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 21 22 23 24

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 05 moderate expression: see section 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 21 22 23

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 04 22 23

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 05 21 22 23 24

clavicle

strong

regional

strong expression: see section 11 12 17 18

axial skeleton

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

nose

moderate

regional

moderate expression: see section 09 10 11 12 13 14 17 18 19 20 21 22

testis

strong

regional

strong expression: see section 06 07 08 09 21 22 23 24

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 04 05 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4707
Entity Detected:	Mmp8, matrix metallopeptidase 8 ( MGI:1202395)
Sequence:	sense strand is shown >T4707 CAGTGATTGGCAGGGGATGCTGTGAGCTTGAGGGCACTTGCCAGAGGGGCAGAGGAAGGCAGGAGAGGTT GTCTTGGAAGATAAGATTATGTTTCGCCTGAAGACACTTCCATTACTGATCTTCCTCCACACACAGCTTG CCAATGCCTTCCCAGTACCTGAACACCTGGAAGAGAAAAATATAAAAACTGCTGAGAATTACCTACGAAA ATTCTACAACTTACCAAGCAATCAATTCCGGTCTTCGAGGAATGCCACGATGGTTGCAGAGAAGCTTAAA GAGATGCAGCGCTTCTTCAGCTTAGCAGAGACTGGGAAGTTAGATGCAGCTACAATGGGGATTATGGAAA TGCCTCGATGTGGAGTGCCTGACTCTGGTGATTTCTTGCTAACCCCAGGAAGTCCCAAGTGGACACACAC TAACCTGACCTACAGGATTATAAACCATACGCCACAGCTGTCAAGGGCTGAAGTGAAAACAGCAATTGAG AAAGCTTTTCACGTCTGGAGTGTAGCATCGCCCCTGACCTTCACCGAGATCTTACAGGGAGAAGCAGACA TCAACATTGCTTTCGTCTCAAGAGACCATGGTGACAATTCTCCATTTGATGGACCCAATGGAATCCTTGC CCATGCCTTTCAACCAGGCCAAGGTATTGGAGGAGATGCTCATTTTGATTCAGAAGAAACGTGGACTCAA GATTCCAAAAATTACAACCTATTTCTCGTGGCTGCTCATGAATTTGGACATTCTTTGGGACTCTCTCACT CCACTGATCCTGGTGCCTTGATGTACCCAAACTATGCTTACAGGGAACCCAGCACCTATTCACTACCTCA AGATGATATCAATGGCATTCAGACAATCTATGGACCTTCAGACAACCCCATCCAACCTACTGGGCCCAGC ACACCCAAAGCCTGTGACCCCCACCTGAGATTTGATGCTACTACCACACTCCGTGGGGAGATTTACTTCT TTAAAGACAAGTACTTCTGGAGACGGCATCCTCAGCTGAGAACAGTTGACCTCAATTTCATATCTCTGTT CTGGCCCTTCCTACCCAACGGTCTTCAGGCTGCTTATGAAGATTTTGACAGAGACCTAGTTTTCCTATTT AAAGGCAGACAGTACTGGGCTCTAAGTGGCTATGACCTGCAGCAAGGTTACCCCAGAGATATATCCAACT ATGGATTCCCAAGGAGTGTCCAAGCCATTGATGCAGCTGTTTCCTATAACGGGAAGACATACTTCTTCAT AAACAACCAGTGCTGGAGATATGACAATCAAAGAAGATCCATGGACCCAGGTTACCCCAAAAGCATACCA AGCATGTTCCCAGGAGTAAACTGTAGAGTCGATGCAGTTTTCCTGCAGGACTCCTTCTTCCTCTTCTTCA GTGGACCACAATATTTTGCATTTAATTTTGTCAGTCACAGAGTCACTAGAGTTGCAAGAAGCAATTTATG GCTTAACTGTTCATAGAGTTGAGCAATGTCAAATGTCCTTGCACGCACCCTATGAGGACAAAAAGGGAAG CTCAGTCTGTATACTCATCCCACGTGTCCTGCCCAGACTTTCCTCAGTGGTAACTAACTCTGCAGCCCTC TTCGAAGAACCAGCGTTTGTAATCTGGCATTCCCAGCCATGGCTAACTCCTTCTGCATATTCCGTCCCTG GTTCGTGAATGCATCACAAAGAGAGCAAGCCGTTTACTACCTTACTCTTCACTATTTCATTATCTGCTCT TCTGCCTTCTAAAACCTTTTGTTTTATTACTTGCTTATCTAGCTTCAAATGTTCCTAAATCTCTTCAAAC AGGGAATCTAGCATTGGATTTTCATTACAAATGAAGATTATAAACACATAGATATTATCCCCTTATCTGT ACAGAAAAAAAGTGCTGTTATTTCCTCCGTTGGAAAAATATGAAAAATATGTAATGTGTGTTGAATCATG CCCCTCTGCATGCCAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5032770 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5032770 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003084 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source