EMAGE mouse gene expression spatial database

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EMAGE:31613

Col13a1 collagen, type XIII, alpha 1 ( MGI:1277201)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003080_01	euxassay_003080_02	euxassay_003080_03	euxassay_003080_04	euxassay_003080_05

euxassay_003080_06	euxassay_003080_07	euxassay_003080_08	euxassay_003080_09	euxassay_003080_10

euxassay_003080_11	euxassay_003080_12	euxassay_003080_13	euxassay_003080_14	euxassay_003080_15

euxassay_003080_16	euxassay_003080_17	euxassay_003080_18	euxassay_003080_19	euxassay_003080_20

euxassay_003080_21	euxassay_003080_22	euxassay_003080_23	euxassay_003080_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 13 14 16 17 18 19 20 moderate expression: see section 10 11 12 15 21 weak expression: see section 07 08 09 22 23 24

meckel's cartilage

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 21 22 23 24

lower jaw molar

strong

regional

strong expression: see section 08 09 10 11 12 21 22 23 24

upper jaw molar

strong

regional

strong expression: see section 08 09 10 11 12 21 22 23 24

tail mesenchyme

moderate

regional

moderate expression: see section 14 15 18 19

vibrissa

moderate

regional

moderate expression: see section 07 08 10 11 12 24

lower jaw incisor

strong

regional

strong expression: see section 13 14 15 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 13 14 15 16 19 20

glans of male genital tubercle

moderate

regional

moderate expression: see section 14 15 16 17 18

rib

strong

regional

strong expression: see section 04 05 06 07 08 09 10 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4673
Entity Detected:	Col13a1, collagen, type XIII, alpha 1 ( MGI:1277201)
Sequence:	sense strand is shown >T4673 CCCACGCGTCCGGCGCGGCTCACTGCAGCCACGACCCACCTCTGCCCATGGGGCCCTGCATGCGTGCCCC TTCGCCCGGGGACTGACACCCGTTCTCCCCGGAGACACAAAACACCCAGAAGGACCCCGAGCGTCGCACC AGCCCGAGAACTCTGCAGAAGAGAGAAGGGAAAGAGTGGGGAGCGGGGCCAGATCCCCGAACTTGGAACA TTCTTCGAAATAACTTTTTTCTTACTTTGGCGTACTCCGATCGCGTCTGCTGGTGGCTCTTGGCTGCTCC CGACTCCGACTGCTCATTTTCGGTCCTCTTTGGCGAGCAAAGAAAAGGGACGTCCTCTAGCCACGTTAAC CCCCTTTCCCCTTGCCTCTCAGTTTGGATCGCGCCTCTTGGATCTCGTTTTTATTTATTCTATTTATTTA TTTATTGGCTCTCAAGACGCGAGGGCATGGTGGCGGAGCGCACCCGCAAAGCGGCAGCCTCTGGCAGCCG CGGCCCTGGGGAGCTGGGCGCTCCGGGGCCCGGGACAGTGGCGCTGGCTGAGCAGTGCGCTAGGCTGCCG AGCCCCGGGTGCTGCGGACTGCTGGCTCTAGCGCTGTGTTCGCTGGCGCTCAGCCTGCTCGCCCACTTTC GGACCGCGGAGCTGCAGGCCCGGGTGCTGCGCTTGGAAGCAGAGCGCGGGGAGCAGCAGATGGAGAAAGC TATTTTGGGACGAGTCAACCAACTGCTGGACGAGAAATGGAAGTTCTACTCGCGTAGGCGCCGGGAAGCC CCGAAGATGTCTCCAGGATGTAACTGCCCACCAGGACCTCCTGGTCCCACTGGAAGACCTGGACTCCCAG GGGACAAGGGTGCCATCGGGATGCCTGGACGTGTGGGTGCCCCTGGAGATGCGGGGATGTCCATCGTCGG GCCTCGAGGCCCCCCTGGCCAGCCTGGTACCCGAGGTTTCCCTGGATTCCCGGGTCCCATTGGTCTTGAC GGCAGGCCGGGCCACCCCGGACCTAAAGGGGAGATGGGCCTGGTGGGTCCTCGAGGACAACCGGGACCTC AGGGACAAAAGGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACAGGGAGTACCCAGGCGGCATGCTAGC AGCTCTGCGCTCCAACCCGATAATGTCCCTAAAGGGGGAACAAAGTCAGACTGGCATCCAAGGCCCTCCA GGGCCACCGGGCCCCCCAGGACCAAGTGGCCCTCTGGGACACCCAGGACTGCCAGGGCCTATAGGGCCAC CTGGTTTACCGGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTATCATGGCCGCAAGGGAGA GCGGGGCATGCCAGGAATGCCAGGCAAGCACGGAGCCAAGGGGGTTCCTGGGATTGCCGTGGCTGGGATG AAGGGTGAGCCAGGGACGCCAGGAACCAAGGGTGAGAAGGGTGCAGCAGGCTCCCCTGGCCTCCTGGGGC AGAAGGGAGAAAAAGGTGATGCTGGCAATGCCATCGGAGGAGGCAGGGGGGAGCCTGGCCCCCCGGGGCT CCCTGGGCCCCCTGGGCCAAAGGGAGAAGCTGGTGTTGATGGCCAGGCTGGGCCCCCAGGACAGCAAGGA GACAAGGGGCAGCCAGGAGCAGCTGGTGAACAGGGACCCTCTGGCCCCAAGGGTGCCAAGGGTGAACCCG GCAAAGGAGAGATGGTGGATTATAATGGCAGCATCAACGAGGCGCTTCAGGAGATCAGGACGCTGGCCTT GATGGGGCCTCCTGGTCTTCCTGGACAAACAGGCCCACCCGGACCTCCAGGGACTCCAGGCCAAAGGGGG GAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGACAAGGGGCCTCGAGGAAAACCAGGAGATGGGG CCGGCTGGTCCTCAAGGACCCCCAGGAAAGGATGGGCCTCCAGGAATGAAGGGAGAAGTCGGACCCCCAG GAAGTCCAGGGGAGAAAGGGGAGACAGGACAAGCAGGCCCCCAGGGAGAGAAAGGAGAAGCTGGGGAGAA AGGCGACCCGGGAGCAGAGGTTCCTGGGCCGCCAGGGCCAGAGGGTCCTCCAGGACCTCCGGGCCTCCAA GGTTTTCCTGGACCAAAGGGAGAAGCAGGCCTGGAGGGCAGTAAAGGAGAGAAGGGCTCCCAGGGAGAAA AAGGAGACCGGGGGCCTCTGGGATTGCCTGGTACTCCAGGACCAATCGGGGTTCCAGGCCCAGCCGGACC AAAGGGCGAAAGGGGCAGCAAAGGCGACCCAGGGATGACGGGACCAACGGGAGCAGCTGGGCTGCCTGGT TTACATGGACCACCCGGGGACAAAGGAAACCGGGGGGAGAGGGGGAAGAAGGGCTCTAGAGGGCCTAAAG GGGACAAGGGGGACCAAGGAGCACCTGGACTAGACGCCCCCTGCCCGCTGGGAGAAGATGGCTTACCAGT CCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTGGGACTGGCCTGTGTGTGTTTGTATATAGGATATTT ATTTTTATACAGTTTTCACCTTTTGAAAATGCCCAAACTATGATGCATCTTACAGATCATTAAGAAAGAA AGAAGAAAAGCCTGCAGATTTTGTACAGGAAATAACCCCTTTCCCTTTTTGTTTACAAGACATTTTTGTA TAAATCTGTTTCTAATACACTTTCTGTCTGTGCGTCTGCTCCTAATGTTGCATGATATTTGTGCACACTT ACTAAATATCACAGCTTCATAAATTATTGTGTTCCAGTGCCAAAGGGGGCCCACCAGCACAGAGGTGCTG TGAGCTTGTGTGTGAATTCACACACACACACAAAAAAAGTGGAGGTCGGTGGTGTTTGCCAGACTACATG TGTCTGACAATATTTTACATTTTAACTGACGGTCATTATTATAAGAAAAAAATAATAAAACGTGGCAATT CAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5025449 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5025449 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003080 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source