EMAGE mouse gene expression spatial database

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EMAGE:31617

Sorcs1 VPS10 domain receptor protein SORCS 1 ( MGI:1929666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005027_01	euxassay_005027_02	euxassay_005027_03	euxassay_005027_04	euxassay_005027_05

euxassay_005027_06	euxassay_005027_07	euxassay_005027_08	euxassay_005027_09	euxassay_005027_10

euxassay_005027_11	euxassay_005027_12	euxassay_005027_13	euxassay_005027_14	euxassay_005027_15

euxassay_005027_16	euxassay_005027_17	euxassay_005027_18	euxassay_005027_19	euxassay_005027_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

regional

moderate expression: see section 01 02 03 19 20

spinal cord mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08

olfactory cortex ventricular layer

strong

regional

strong expression: see section 09 10 13 14

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 01 02 03 08 09 10 11 12 13 14

urethra of male

moderate

regional

moderate expression: see section 09 10

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11

pons mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13

bladder

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11

hypothalamus mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 08 09 10

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 12 13 14 15 16 17

frenulum

moderate

regional

moderate expression: see section 09 10 12 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18

telencephalon marginal layer

moderate

regional

moderate expression: see section 09

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 10 12 13 14 15 16 17 18 19 20

midbrain mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

glans of male genital tubercle

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6512
Entity Detected:	Sorcs1, VPS10 domain receptor protein SORCS 1 ( MGI:1929666)
Sequence:	sense strand is shown >T6512 GTTTGCAGCCGTTCAAGAATGGAACCAGAACGACACATACAACCTGTACATCTCAGACACACGAGGAGTC TACTTCACACTGGCCTTGGAGAATGTGCAGAGCAGCAGAGGCCCTGAAGGCAACGTCATGATTGACCTTT ATGAGCAGGGATAAAGGGAATGTTCTTGGCTAACAAGAAGATTGACAACCAAGTGAAGACTTTCATCACT TACAACAAAGGCAGAGACTGGCGGTTGCTGCAGGCTCCAGATGCAGATCTAAGGGGGGACCCTGTGCACT GTTTGCTGCCCTACTGTTCGCTACACCTTCACCTGAAGGTTTCTGAGAATCCCTACACATCCGGGATCAT TGCCAGCAGAGACACAGCCCCAAGTATTATAGTTGCATCAGGTAACATAGGCTCTGAGTTGTCAGACAGT GACATCAGCATGTTTGTATCTTCAGATGCAGGGAATACTTGGAGGCAGATTTTTGAAGAAGAGCACAGCA TTTTGTACCTTGACCAAGGTGGAGTCCTTGTTGCTATGAAACACACATCTCTCCCAATTCGACACCTTTG GTTGAGTTTTGATGAAGGGAGATCTTGGAGCAAATACAGCTTTACATCCATTCCACTCTTTGTGGATGGA GTCCTGGGGGAGCCTGGAGAAGAGACACTAATCATGACAGTGTTTGGACACTTCAGTCACCGTTCCGAAT GGCAGCTGGTCAAAGTGGACTACAAGTCCATTTTTGATAGACGCTGTGCTGAAGAAGACTACAGACCCTG GCAGTTGCATAGTCAGGGGGAAGCATGCATCATGGGAGCCAAGAGGATATACAAGAAGAGAAAGTCTGAG CGCAAGTGTATGCAAGGAAAGTATGCGGGAGCAATGGAGTCTGAGCCTTGTGTTTGTACAGAGGCAGACT TTGACTGTGACTATGGCTATGAGAGACATAGTAATGGACAATGCCTGCCAGCATTTTGGTTCAACCCATC CTCACTGTCAAAAGACTGCAGCTTGGGACAGAGTTACCTCAACAGCACTGGGTATAGGAAGGTGGTCTCC AATAATTGTACTGATGGAGTGAGGGAGCAGTATACCGCCAAACCACAGAAGTGTCCAGGGAAGGCCCCTC GTGGGCTGCGGATTGTCACTGCAGATGGGAAACTGACAGCAGAGCAGGGCCACAATGTCACTCTCATGGT GCAGCTGGAAGAGGGTGATGTGCAGCGGACACTCATCCAAGTGGACTTCGGAGATGGCATTGCAGTATCG TATGTCAACCTCAGCTCTATGGAAGACGGGATCAAGCACGTATATCAGAATGTGGGCATTTTCCGAGTGA CCGTGCAGGTGGACAACAGTCTGGGGTCTGACAGCGCCGTCCTGTACTTACATGTAACTTGTCCCCTGGA GCATGTGCACCTGTCTCTTCCTTTTGTCACAACAAAGAACAAAGAGGTCAACGCAACTGCAGTGCTTTGG CCCAGCCAAGTGGGCACTCTCACTTACGTGTGGTGGTATGGGAACAACACAGAGCCCCTGATCACCTTGG AGGGAAGCATATCGTTCAAGTTTACTTCTGAAGGAATGAATACTATCACAGTCCAAGTCTCTGCTGGGAA TGCCATACTGCAAGACACAAAGACCATTGCGGTATACGAGGAATTTCGGTCTCTTCGCTTGGCCTTTTCT CCAAACTTGGATGACTACAACCCTGACATCCCGGAGTGGAGGAGGGACATCAGCCGAGTCATCAAAAAGT CTCTGGTGGAAGCCACAGGGATTCCCAGCCAGCATATCCTGGTGGCGGTGTTACCTGGCTTACCCACTGC TGCAGAGCTCTTTGTTTTGCCCTACCAAGATGGAACCAGAGAAAACAAAAGGTCGTCTGAGGACCTGGAG CAGATATCTGAAGTTCTAATCCACAAACTCAACCAAAACTTGGTGCACTTCGAGCTGAAGCCTGGGGTCC AAGTTCTTGTCCACGCAGCTCACCTGACAGCAGCTCCACTGGTGGACCTCACTCCTACCCACAGCGGATC TGCCATGCTGATGTTGCTCTCTGTGGTGTTTGTGGGGCTTGCTGTATTCGTCATCTACAAGTTTAAAAGG AAAATTCCCGGGATTAATGTTTATGCCCAGATGCAAAATGAGAAAGAACAAGAGCTGATCAATCCAGTCA GCCACTCTGAAAGCAGGTCCAGTGTCCCTCACCCTGACCTCAGGCGGCCTGGCCAGCTTGTAGATGAGAA GGTGGAATCCCAGCTCCTAGGAGAGTAGCTTTACCCTCCCCTCCCTCCCCTTCTGCTCAACCTGGTGACT CATCTCTCCGATTGCAAAGACCAAGACCAGCAACTCCACCTTCATCGCCAAAGCGGGGATCTGCTGGGGC ACAGTTTGCAATTTAAGGAAAAACCCCCAAAGGCTACAGGAGACCTGCTGATCAGAAGAGAATTTCGCTC TTGTCAAGTGCATCATCCCTCATGACCACTAACTTTATGTTTTGTTTTGTTTTCTTCTTCTGTTGTTCTG TTTCCTATTTTGCCAGGAAGTATTTCCATAGTTGCTGAGAATCAAAGCACAAAAAAAATCCCTACCTATG TAAATGTTTGAATGGAGGACGCCAGTAAAACAAACAAAACAAACAAACAAATAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590084 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590084 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005027 same experiment
	EMAGE:31702 same embryo
	EMAGE:31692 same embryo
	EMAGE:29645 same embryo
	EMAGE:31700 same embryo
	EMAGE:31688 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source