EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31619

Srgn serglycin ( MGI:97756)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003087_01	euxassay_003087_02	euxassay_003087_03	euxassay_003087_04	euxassay_003087_05

euxassay_003087_06	euxassay_003087_07	euxassay_003087_08	euxassay_003087_09	euxassay_003087_10

euxassay_003087_11	euxassay_003087_12	euxassay_003087_13	euxassay_003087_14	euxassay_003087_15

euxassay_003087_16	euxassay_003087_17	euxassay_003087_18	euxassay_003087_19	euxassay_003087_20

euxassay_003087_21	euxassay_003087_22	euxassay_003087_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15

liver lobe

moderate

regional

moderate expression: see section 04 05 06 07 08 11 12 13 14 15 18 19 21 22 23 weak expression: see section 01 02 03 09 10 16 17 20

dorsal grey horn

moderate

regional

moderate expression: see section 11 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4733
Entity Detected:	Srgn, serglycin ( MGI:97756)
Sequence:	sense strand is shown >T4733 CCACGCGTCCGGTGGAGCCGAGCTGGTCAGGATGCAGGTTCCCGTCGGCAGCAGGCTTGTCCTGGCTCTC GCCTTCGTCCTGGTTTGGGGATCTTCAGTGCAAGGTTATCCTGCTCGGAGAGCCAGGTACCAGTGGGTCC GCTGCAAACCGAATGGCTTTTTTGCGAACTGCATCGAGGAGAAGGGACCACAGTTTGACCTAATAGATGA ATCCAATAACATCGGCCCTCCCATGAATAATCCTGTTTTGATGGAAGGACCCTCAAAAGATTTCATCTCC AATTATGATGACTATGGGTCAGGTTCGGGCTCCGGCTCTGGCTCCGGCTCTGGCTCGGGTTCCGGCTCCG GAAGTGGCTTCCTAGGTGACATGGAATGGGAATACCAGCCAACAGATGAAAGCAATATTGTCTATTTCAA CTATAAGCCTTTTGACAGGATTCTCACTGAGCAAAACCAAGACCAACCAGAAGACGATTTTATTATATGA ATGTGACGGTCTCTGTCTCCCCACCTCCATGTGGAACAATGTATTCAGTATACTTAGTGTACCACGTTTA AATGACCAGTCTCAGGATAAAGAGTTTTACAGAAAATTTAAAATGCCTGGAAAAGACTCTTGAATCCTGT TACCCCTTTCCTCATTAACTCATAAGGAATTATGCTTTAATGCTGTTACCTATCTTGTTGTTCTGGAAAA TGCCTGCATTTATGTGTATTGAATCAACATTTAAGAAATTAACACACACCCCCATTATTATACAATAACT TTCAAAGCCATACTGGTTTTGAAAATTTTAATTTGATAGCAAGTTGATGAACAATCTTTCATACCTAAAG TGTTCAGGAACCCAACTCGCATTGTGAATTACAAATATATTCCTTTATGTGATTAAAAAGAAAATAAAGT GAAACACAGTGGCCTCCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5038568 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5038568 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003087 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source