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EMAGE:31626

Runx3 runt related transcription factor 3 ( MGI:102672)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626
euxassay_019644_01 euxassay_019644_02 euxassay_019644_03 euxassay_019644_04 euxassay_019644_05
EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626
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EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626
euxassay_019644_11 euxassay_019644_12 euxassay_019644_13 euxassay_019644_14 euxassay_019644_15
EMAGE:31626 EMAGE:31626 EMAGE:31626 EMAGE:31626
euxassay_019644_16 euxassay_019644_17 euxassay_019644_18 euxassay_019644_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 08 09 10
humerus
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 03
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 07 08 09 10
clavicle
strong strong
regionalstrong expression: see section 09 10 14 15 16 moderate expression: see section 05 06 07 08
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 10 weak expression: see section 11 12
forelimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 03 04 05
axial skeleton
moderate moderate
regionalmoderate expression: see section 06 07 08 10 14 15 16
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 15 16 17
viscerocranium
strong strong
regionalstrong expression: see section 19 moderate expression: see section 18
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 09 10
thymus primordium
strong strong
single cellstrong expression: see section 13 moderate expression: see section 10 11 12
trigeminal v ganglion
strong strong
single cellstrong expression: see section 03 04 05 06 07 08 15 16 17 18 19
tibia
strong strong
regionalstrong expression: see section 05 moderate expression: see section 04
femur
moderate moderate
regionalmoderate expression: see section 03 06 07 weak expression: see section 04 05 08
vibrissa
strong strong
regionalstrong expression: see section 05 06 moderate expression: see section 07 08 18 19
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
orbito-sphenoid
strong strong
regionalstrong expression: see section 03 04 05 19 moderate expression: see section 01 02 18
cornea epithelium
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 04
mandible
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08
fibula
strong strong
regionalstrong expression: see section 05 moderate expression: see section 03 04
rib
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 04 05 06 07 08 09 17 18 19
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08
dorsal root ganglion
strong strong
single cellstrong expression: see section 07 08 09 10 11 12 13 14 15 16
forelimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05
basioccipital bone
moderate moderate
regionalmoderate expression: see section 10 11 12 13
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 10 11 12 13
scapula
moderate moderate
regionalmoderate expression: see section 19
maxilla
strong strong
regionalstrong expression: see section 03 05 06 07 08 09 13 14 15 16 17 18 19
lower jaw incisor
strong strong
regionalstrong expression: see section 10 11 12
upper jaw incisor
strong strong
regionalstrong expression: see section 10 11 12
glans of male genital tubercle
moderate moderate
regionalmoderate expression: see section 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55171
Entity Detected:Runx3, runt related transcription factor 3 ( MGI:102672)
Sequence:sense strand is shown

>T55171
AACTTCCTCTGCTCCGTGCTGCCCTCGCACTGGCGCTGCAACAAGACGCTGCCGGTCGCCTTCAAGGTGG
TGGCCCTGGGGGATGTGCCGGATGGAACGGTGGTGACCGTGATGGCCGGCAATGATGAGAACTACTCCGC
CGAGCTGCGCAACGCTTCCGCTGTCATGAAGAACCAAGTGGCCAGGTTCAACGACCTTCGATTCGTGGGC
CGCAGTGGGCGAGGGAAGAGTTTCACGCTCACAATCACCGTGTTCACCAACCCTACCCAAGTGGCTACCT
ACCACCGAGCCATCAAGGTCACTGTGGATGGACCCCGGGAACCCCGACGGCACCGGCAGAAGATAGAAGA
CCAGACCAAGGCCTTCCCCGACCGCTTTGGAGACCTGCGCATGCGTGTAACACCAAGCACACCCAGCCCC
CGTGGCTCTCTCAGCACCACGAGCCACTTCAGCAGCCAGCCCAAGACCCCAATCCAAGGCTTCTTAAACC
TGAACCCCTTCTCCGACCCCCGCCAGTTTGACCGCTCCTTCCCTACGCTGCAGAGCCTCACAGAGAGCCG
CTTCCCGGACCCCAGGATGCACTACCCGGGAGCCATGTCTGCCGCCTTCCCCTACAGCGCCACACCATCG
GGCACCAGCCTGGGCAGCCTGAGCGTGGCGGGCATGCCGGCCAGCAGCCGCTTCCACCACACCTACCTCC
CTCCGCCCTACCCCGGGGCCCCACAGAGCCAGAGCGGGCCCTTTCAGGCCAACCCCGCGCCCTACCACCT
CTTTTACGGCGCCTCCTCCGGCTCCTACCAGTTCTCCATGGCAGCCGCGGGAGGTGGTGAGCGCTCGCCC
ACCCGCATGCTGACCTCCTGCCCCAGCGGCGCTTCGGTGTCAGCAGGCAACCTCATGAACAACTTCCTCT
GCTCCGTGCTGCCCTCGCACTGGCGCTGCAACAAGACGCTGCCGGTCGCCTTCAAGGTGGTGGCCCTGGG
GGATGTGCCGGATGGAACGGTGGTGACCGTGATGGCCGGCAATGATGAGAACTACTCCGCCGAGCTGCGC
AACGCTTCCGCTGTCATGAAGAACCAAGTGGCCAGGTTCAACGACCTTCGATTCGTGGGCCGCAGTGGGC
GAGGGAAGAGTTTCACGCTCACAATCACCGTGTTCACCAACCCTACCCAAGTGGCTACCTACCACCGAGC
CATCAAGGTCACTGTGGATGGACCCCGGGAACCCCGACGGCACCGGCAGAAGATAGAAGACCAGACCAAG
GCCTTCCCCGACCGCTTTGGAGACCTGCGCATGCGTGTAACACCAAGCACACCCAGCCCCCGTGGCTCTC
TCAGCACCACGAGCCACTTCAGCAGCCAGCCCAAGACCCCAATCCAAGGCTTCTTAAACCTGAACCCCTT
CTCCGACCCCCGCCAGTTTGACCGCTCCTTCCCTACGCTGCAGAGCCTCACAGAGAGCCGCTTCCCGGAC
CCCAGGATGCACTACCCGGGAGCCATGTCTGCCGCCTTCCCCTACAGCGCCACACCATCGGGCACCAGCC
TGGGCAGCCTGAGCGTGGCGGGCATGCCGGCCAGCAGCCGCTTCCACCACACCTACCTCCCTCCGCCCTA
CCCCGGGGCCCCACAGAGCCAGAGCGGGCCCTTTCAGGCCAACCCCGCGCCCTACCACCTCTTTTACGGC
GCCTCCTCCGGCTCCTACCAGTTCTCCATGGCAGCCGCGGGAGGTGGTGAGCGCTCGCCCACCCGCATGC
TGACCTCCTGCCCCAGCGGCGCTTCGGTGTCAGCAGGCAACCTCATGAAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019644 same experiment
 EMAGE:31519 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS