EMAGE mouse gene expression spatial database

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EMAGE:31633

Actr1a ( MGI:1858964)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006567_01	euxassay_006567_02	euxassay_006567_03	euxassay_006567_04	euxassay_006567_05

euxassay_006567_06	euxassay_006567_07	euxassay_006567_08	euxassay_006567_09	euxassay_006567_10

euxassay_006567_11	euxassay_006567_12	euxassay_006567_13	euxassay_006567_14	euxassay_006567_15

euxassay_006567_16	euxassay_006567_17	euxassay_006567_18	euxassay_006567_19	euxassay_006567_20

euxassay_006567_21	euxassay_006567_22	euxassay_006567_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 16 17 18

facial vii ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 19 20 21 22

vagus x ganglion

strong

homogeneous

strong expression: see section 07 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22 23

cervical ganglion

strong

regional

strong expression: see section 08 09 16 17

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 05 06 07 17 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 11 12 15 16

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 05 06 18 19

trigeminal v nerve

strong

regional

strong expression: see section 09 10 17

thoracic ganglion

strong

regional

strong expression: see section 12 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6636
Entity Detected:	Actr1a, ( MGI:1858964)
Sequence:	sense strand is shown >T6636 CGGACGCGTGGGCGGACGCGTGGGTGCGGACCGGGCTGGCAGTTCCTTCCTCGGAGGGAAAGATTCCTCT GCCATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTTGTGATCGACAACGGATCCGGTGTGATTAAAG CTGGCTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGCAGACCCAAACATGTTCG TGTCATGGCAGGAGCCCTGGAAGGTGACATCTTCATTGGCCCCAAAGCGGAGGAGCACCGGGGGCTGCTG TCAATCCGTTACCCCATGGAACATGGCATCGTCAAGGACTGGAACGACATGGAACGCATCTGGCAATACG TCTACTCTAAGGACCAGCTGCAGACTTTCTCAGAGGAGCATCCTGTGCTCCTAACGGAGGCACCTTTAAA CCCTCGAAAAAACCGGGAGCGAGCTGCAGAAGTCTTCTTCGAGACCTTCAATGTGCCGGCCCTCTTCATC TCCATGCAAGCCGTGCTGAGCCTTTATGCCACGGGCAGAACCACAGGCGTGGTGTTGGATTCTGGGGATG GTGTCACCCATGCAGTCCCGATTTATGAAGGCTTCGCCATGCCTCACTCCATCATGCGCATCGACATCGC TGGCCGGGATGTCTCGCGCTTCCTCCGCCTCTACCTACGGAAGGAGGGCTATGATTTCCACTCCTCCTCT GAGTTTGAGATTGTCAAGGCCATAAAGGAAAGAGCTTGCTACCTGTCCATAAACCCCCAGAAGGATGAGA CACTGGAGACAGAGAAGGCTCAGTACTACCTGCCCGATGGCAGCACCATTGAGATTGGCCCTTCCCGGTT CCGGGCCCCTGAGCTGCTGTTCAGGCCGGACTTGATTGGCGAGGAGAGTGAAGGCATCCACGAGGTGCTG GTGTTTGCTATCCAGAAGTCAGACATGGATTTACGGCGTACACTGTTTTCTAACATTGTCCTCTCAGGAG GTTCTACCCTGTTCAAAGGTTTTGGAGACAGGCTACTGAGTGAAGTGAAGAAACTAGCTCCAAAAGACGT GAAGATCAGGATATCTGCACCCCAGGAGAGACTGTATTCCACATGGATTGGAGGTTCTATCCTTGCTTCC CTGGACACCTTTAAGAAGATGTGGGTCTCTAAGAAAGAATATGAAGAAGACGGTGCCCGATCCATCCACA GGAAAACCTTCTAACGTTGCAACATCACCACCTCTCTCTGAAGTTAACTCCACTTTAAAACTCGCTTTCT TGAGTTGGAGTGTTTGAGAGGAACTGCCTGTGTGTGTGAATGAGTGTGTGGGTATGTATGAGTGTGTGAG ACTATCGAGTGCCGTGTGGCCCAGAGATCCTGGGCCCAGAGAGGACATTGAACTACCCACGATGGTGACG GCCTGAGGCCTGGGGTTGACTACTAACTGACCCCTTACAGGAAGAGCTCTGGCAGAGGCCCACTCCTCTC TCAGCCAGGCCTTTGTCTGGCTGTGCGGAACTATGGTTACTACCATAGAGAGACCTTGCTGCTGCCGATC TAGGCTGGGCTGGCCCCACCCCACCCCTCCCCACCCGAGTGCCCTGAGGTCTGAGGCAGCTGCTGTCTCC CCTGCTCATCCTTCTGTGCTGTCCATGCCTCTGGGGGGCGGGGTTAAGTTCTGCCCGGTTTTGTTTCAGT TTGGGTTTGGGAGACTGAAAAAGCAGTCTAGCCGTGGTTAGAGACTGGGTTCAAGAGAGAGCAGGGTACA CTCGTGTCCACCTGGGAACCAGGTAGGGATTAGGACCACACTGGGCATTTGAGGACAGGAGATAGCTTCA GGGTTAATTTACAAGCCCCAGCCACATCCAGGGGCCCTGAGACACTGTAAGGACTGTGGGCTGCAGGTCC TGCTGTTGGCTTCTCCTACGTCCTGGTTTCGATGCCATCATCACGGTTTTATTTATTGACGTGTTTGTTC AGTAAAGGGACTACAGAGAGGGCTCGCTGTCTCCTACCCACCAGTCACCTTGCTTACACTAATGTTTACA GCACCTAGGTTTCCCATAGCATCCAGGCCTCTGTCATTCCCTGCTGATGGAGAACTGACAGTATTCATGG GCCTCAGGACCCTGTGATTGGAGGCTGCACAGGAAGGCACTCCAGCCAGCCTCTTCCTTTCTCCTAACCC CACTTGCCTACTGCCCTGGAACCAACAGGCTGGTTGTGGTTGGATTTTCTAAGACAGAAAGTAGCATCTC TCTGTGGCAGAGGTCCCTTGCGAGGGAGGTGTGTTGGAGATCTAGACCCTTTAAAACAAAGCAAGCTGCA GTTAACCATGTTGCCTTTTGCCACTCTAATTGACCAGTGGACTAAATGGCAGGGAGGCCCCTTCTGGGTT GGTCAGATTGAAGAGAAGGGGCTGACCAAAGTGCCAAGACCTAATGGGTGCCAGCCTTGCTCTCAGAAGT GGTCCAAGGGCCTTTGCGCCTGGTACAAGCTAGGGTGAGCCTGTCTTCACCGGCACCCTCACCCGTGACA CCAAGTCTCACCTCAGTTCCAGATTCCAGACAGTATTCTTTCTCCGCCATCCTATGACCAAAGGCCCCTG CCAGATGTAAAGCGAAGCTGGTGTGTGTGGAATCAGTGTGGCAACCCATGGACTGCAGTGCGCATAACCA GCTCACCTCTTAGCCCAAGCCTGTCCCTTGCACAGCCTCGCGAAGCCACTTTGCCTGGTGGGGCCCAGTG TACTTAAATAAAGTCGTTCCAATAGGTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599656 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599656 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006567 same experiment
	EMAGE:31924 same embryo
	EMAGE:29629 same embryo
	EMAGE:29620 same embryo
	EMAGE:31600 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source