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EMAGE:31639

Tyro3 ( MGI:104294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
cerebral cortex marginal layer
moderate moderate
regionalmoderate expression: see section 05 18 19 weak expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17
medulla oblongata basal plate
moderate moderate
regionalmoderate expression: see section 05
vagus x ganglion
weak weak
regionalweak expression: see section 05 06 15 16
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 12 13 15 16 17 moderate expression: see section 09 10 11 18 19
naris
weak weak
regionalweak expression: see section 14
seminiferous cord
strong strong
regionalstrong expression: see section 03 04 05 06 17 18
pons
moderate moderate
regionalmoderate expression: see section 03 04 05 17 18 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35011
Entity Detected:Tyro3, ( MGI:104294)
Sequence:sense strand is shown

>T35011
GAATGGGAAGAAGTGATTCCTGAGGACCCTGGGGAAGGCCCCCTAGGACCTTATAAGCTGTCCTGGGTCC
AAGAAAATGGAACCCAGGATGAGCTGATGGTGGAAGGGACCAGGGCCAATCTGACCGACTGGGATCCCCA
GAAGGACCTGATTTTGCGTGTGTGTGCCTCCAATGCAATTGGTGATGGGCCCTGGAGTCAGCCACTGGTG
GTGTCTTCTCATGACCATGCAGGGAGGCAGGGCCCTCCCCACAGCCGCACATCCTGGGTGCCTGTGGTCC
TGGGCGTGCTCACCGCCCTGATCACAGCTGCTGCCTTGGCCCTCATCCTGCTTCGGAAGAGACGCAAGGA
GACGCGTTTCGGGCAAGCCTTTGACAGTGTCATGGCCCGAGGGGAGCCAGCTGTACACTTCCGGGCAGCC
CGATCTTTCAATCGAGAAAGGCCTGAACGCATTGAGGCCACATTGGATAGCCTGGGCATCAGCGATGAAT
TGAAGGAAAAGCTGGAGGATGTCCTCATTCCAGAGCAGCAGTTCACCCTCGGTCGGATGTTGGGCAAAGG
AGAGTTTGGATCAGTGCGGGAAGCCCAGCTAAAGCAGGAAGATGGCTCCTTCGTGAAAGTGGCAGTGAAG
ATGCTGAAAGCTGACATCATTGCCTCAAGCGACATAGAAGAGTTCCTCCGGGAAGCAGCTTGCATGAAGG
AGTTTGACCATCCACACGTGGCCAAGCTTGTTGGGGTGAGCCTCCGGAGCAGGGCTAAAGGTCGTCTCCC
CATTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50110. Forward Primer - name:050110_F_cDNA_Tyro3, sequence:GAATGGGAAGAAGTGATTCCTG; Reverse Primer - name:050110_N_SP6_cDNA_Tyro3, sequence:GGAATGGGGAGACGACCTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006322 same experiment
 EMAGE:31616 same embryo
 EMAGE:32100 same embryo
 EMAGE:31572 same embryo
 EMAGE:31573 same embryo
 EMAGE:30469 same embryo
 EMAGE:30898 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS