EMAGE mouse gene expression spatial database

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EMAGE:31647

Stc1 stanniocalcin 1 ( MGI:109131)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003067_01	euxassay_003067_02	euxassay_003067_03	euxassay_003067_04	euxassay_003067_05

euxassay_003067_06	euxassay_003067_07	euxassay_003067_08	euxassay_003067_09	euxassay_003067_10

euxassay_003067_11	euxassay_003067_12	euxassay_003067_13	euxassay_003067_14	euxassay_003067_15

euxassay_003067_16	euxassay_003067_17	euxassay_003067_18	euxassay_003067_19	euxassay_003067_20

euxassay_003067_21	euxassay_003067_22	euxassay_003067_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23

renal cortex

moderate

regional

moderate expression: see section 05 06 07 08 09 15 16 17 18 weak expression: see section 19

kidney pelvis

moderate

regional

moderate expression: see section 17

tail mesenchyme

moderate

regional

moderate expression: see section 11 12 14 15 16

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 10 11 16 18 19 20 21 22 23

tongue

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

axial skeleton

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 11

axial skeleton tail region

moderate

regional

moderate expression: see section 13 14

foot mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 weak expression: see section 19 20

testis

moderate

regional

moderate expression: see section 04 05 06 07 18 19 20

dorsal grey horn

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4651
Entity Detected:	Stc1, stanniocalcin 1 ( MGI:109131)
Sequence:	sense strand is shown >T4651 CCACGCGTCCGCTAAGCTTTCAGTATATCCAGATCCACATCTTCACTCAAGCCGGGAGAGGGAAAGAGGA AAGGGGGGGAGGAAAAAAAAAAGCCAACAACTTAGCGGAAACTTCTCAGAGAATGCTCCAAAACTCAGCA GTGATTCTGGCGCTGGTCATCAGTGCAGCTGCAGCGCACGAGGCGGAACAAAATGATTCTGTGAGCCCCA GAAAATCCCGGGTGGCGGCTCAAAATTCAGCTGAAGTGGTTCGCTGCCTCAACAGTGCCCTGCAGGTTGG CTGCGGGGCTTTTGCATGCCTGGAAAACTCCACATGTGACACAGATGGGATGTACGACATTTGTAAATCC TTCTTGTACAGTGCTGCTAAATTTGACACTCAGGGAAAAGCATTTGTCAAAGAGAGCTTAAAGTGCATCG CCAATGGGATCACCTCCAAGGTATTCCTTGCCATTCGGAGGTGTTCGACTTTCCAGAGGATGATCGCCGA GGTGCAGGAGGACTGCTACAGCAAGCTTAACGTTTGCAGCATCGCCAAGCGCAACCCGGAAGCCATCACT GAAGTCATACAGCTGCCCAATCACTTCTCCAACAGATACTACAACAGACTTGTCCGAAGCCTTCTGGAAT GTGATGAAGACACGGTCAGTACAATCAGAGACAGCCTGATGGAGAAGATCGGGCCCAACATGGCCAGCCT CTTCCACATCCTGCAGACAGACCACTGTGCCCAGACACACCCCAGAGCTGACTTCAATAGGAGGCGCACA AATGAGCCACAGAAGCTGAAAGTCCTCCTCAGGAACCTCCGAGGTGAGGGGGACTCTCCCTCACACATCA AACGCACCTCCCAAGAGAGTGCGTAAGCAGGGAGAGGTATTCACAGCCTCACCAAACTAATAGCATTTTA GGGGTGTTGACACACCAACTTTGAGTGTACTGTGCCTGGTTTGATTTTTTTTAAGTAGTACCTATTTTCT ATCCCCCCGTTAAAGAAAAATTGCATGAAACTAGGCTTCCATAATCAATATCCCAACATTCTGCAATGAC AGCATTCTTACCAACAGAATACATGTGTGGTCATTCTGCCTCTCCTCAAGAGAGAATGTACCCTCTTCCA TCCCCCCTCTCTCTGAATTCTTTTCCCAGATCCCTATCTACTCTCCGCAAACACAAACCATTCATGTAAC TACCCAGTCATTGTAATCTGAAAATGTAGATCCCTTTAGAATGGTCACCTGGTAGAGTTAGCCAATACAA AACAACTTTATTTAAATGCATGTCTTAAATGCTCATAAATATGTTAAATGGAATTCGTGTTATGAATCTG TGCTGGCCATGGACGAATATGAATGTCATGTTTGAATTCTTGATCTCTAACGAGTCTTATGGTCTCTATC CTCCAATGTCTAATTTCCTTTCTGACATATTTACCAAATTGCTCAAACCTGGTTCTCCAACCAGACTTTG AGCTAGCATCTTCTTGCATCTAATGAAAAACAAAAAAGCTAACATCTTTATGTACTGTAACTGCTCAGAG CTTTAAAAGTATCTTTAACAATTGTCTTAAAAAACGGAGAATCTTAAGGTCTAACTGTGGAATATAAATA GCTGAAAACTATTGTACTGTACATAAATTCCAGAGGACTCTGCTTAACAGAGCAGTCTATAATAACTTTA TTGCATATAGATTTAGTTTTGTACCTTAGCTTTATTTTCCTTTTCCTGGGAATGGAATAACTATCTCACT TCCAGATATCCACATTCATGCTCCTTGTGGCCTTTTTTATAACTAAGGGGGTAGAAGTAGTTTTAACTCA ACATCAGAACTTAAGATGGGCCTATACTTGACAGGAAAACCCAACAGGTTATCTGAAGGACCCCAGGTAA GACGTTAATCTCCCAGCCCACCTCAACCCGGAGGCTACGTTTGACTTAGATGTATCCTGAAACAGCTCTG TCACATCCAACTGGGAATAACAAGAATCAAAAAGACCATCCCTTTGGGCTTGGACCACTTGGTGTGACAA GGCCTACTATCCCCTTGGAAGTGGCAGTTCTTGGCTCATCGCCTTCCATCAGTGCCTGGCACTCTGGTAA ATGATGGAGTGGGATATTGTTCCACTAAGCCAATCAGGAATGAGTCAATCAGCCTTTGGGTCTTTAGTCC GGGAAACTTGGGCTTAAGGGGGTATGAATAACCCTGGGCTGTCCAGCCTTAATAGACTCCTCTTACATCT TTTGTCCTGTAACATGCTGCCTGCCAAAGTAGTCCTGGCAGCTGGACCATCTCTGTAGGATCTTTAAAAA AAAAGAAAAAAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010981 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010981 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003067 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source