EMAGE mouse gene expression spatial database

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EMAGE:31648

B7galt7 ( MGI:2385269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 23 24 weak expression: see section 06 07 08 22

nucleus pulposus

strong

regional

strong expression: see section 10 11 12 13 14 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4725
Entity Detected:	B7galt7, ( MGI:2385269)
Sequence:	sense strand is shown >T4725 GCTTGAGGACGCACAGCTGCGAGGAGGGAGTCCGGGCAGGGCTTTACCCGAGGACCCCCAGAGCTGGCGG AAGCTGGACCCAGAGCCCCACTGGTGGCCCTTTCCCTGGGCCGGGTCATGCGTTGCCGCAAGTGCCAGCT CTGCCTGTCAGCACTGCTCACACTCCTGGGCCTCAAAGTATACATCGAGTGGACATCCGAGTCCTGGCTT AAAAAGGCTGAACCCCGGGGCGCTCTGCCCAGTCCCACACCACCCAATGCTGAGCCCACTCTGCCCACCA ACCTCTCAGCACGCCTGGGTCAGACTGGCCCACTGTCCTCTGCTTACTGGAACCAGCAGCAGCGGCAGCT GGGAGTCCTGCCCAGTACGGACTGTCAGACTTGGGGGACTGTTGCTGCCTCGGAGATCTTGGACTTCATC CTGTACCCCCAGGAGCTTCGGCGCTTCTTGCTGTCGGCGGCCTGTAGGAGCTTTCCACTATGGCTGCCTG CAGGAGAAGGCAGCCCTGTGGCCAGCTGCTCTGATAAGGATGTACCCTACTTGCTACTGGCTGTCAAATC AGAACCAGGACACTTTGCAGCACGGCAGGCTGTGAGGGAGACCTGGGGCAGCCCAGTTGCTGGGACCCGG TTGCTCTTCCTGCTGGGGTCCCCCCTAGGAATGGGGGGGCCTGACTTAAGATCACTGGTGACGTGGGAAA GCCGGCGCTATGGTGACCTACTGCTCTGGGACTTCCTGGATGTTCCCTACAACCGGACACTCAAGGACCT GCTGCTGCTGACCTGGCTGAGCCACCACTGCCCCGATGTCAATTTTGTCCTGCAGGTTCAGGATGATGCC TTTGTGCACATCCCAGCCCTACTGGAGCACCTGCAGACTCTGCCACCCACCTGGGCCCGCAGCCTCTACC TGGGTGAGATCTTCACCCAGGCCAAACCGCTCCGCAAGCCCGGAGGACCCTTCTATGTGCCGAAGACCTT CTTTGAAGGGGACTATCCAGCCTATGCGAGTGGAGGTGGCTATGTAATCTCAGGACGCCTGGCACCCTGG CTGCTGCAGGCGGCAGCTCGCGTGGCACCCTTCCCCTTTGATGATGTCTACACTGGCTTCTGCTTCCGTG CCCTGGGCTTAGCACCCCGTGCCCATCCAGGCTTCCTCACAGCCTGGCCAGCAGAACGTACCAGGGACCC CTGCGCCGTGCGAGGCCTGCTCTTGGTGCATCCAGTCAGCCCTCAGGACACCATTTGGCTCTGGAGACAT CTGTGGGTCCCAGAGCTCCAGTGCTGACCGGCAGAGACAAGCTGGGGTGGGTGGGTGCTGACCTGGCCTG AGTCTCTCCTAGAGACAAGCTGGGGTGGGTGGGGCTGACCTGGCCTGAGTCTCTCCTAAACTCTTCCTAG CCAAGGTGGCAGACTGTGTTTATCTACTTTATGGTTTTGAAAAATGTGTCCTTCCTACTCCAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5037295 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5037295 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005487 same experiment
	EMAGE:30528 same embryo
	EMAGE:31603 same embryo
	EMAGE:31594 same embryo
	EMAGE:29399 same embryo
	EMAGE:30478 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source