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EMAGE:31652

Kdelr2 ( MGI:1914163)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652
euxassay_004155_01 euxassay_004155_02 euxassay_004155_03 euxassay_004155_04 euxassay_004155_05
EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652
euxassay_004155_06 euxassay_004155_07 euxassay_004155_08 euxassay_004155_09 euxassay_004155_10
EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652
euxassay_004155_11 euxassay_004155_12 euxassay_004155_13 euxassay_004155_14 euxassay_004155_15
EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652 EMAGE:31652
euxassay_004155_16 euxassay_004155_17 euxassay_004155_18 euxassay_004155_19 euxassay_004155_20
EMAGE:31652
euxassay_004155_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
axial skeleton lumbar region
moderate moderate
regionalmoderate expression: see section 11 13 14 15 16 17 18 19
mandible
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20
sternum
moderate moderate
regionalmoderate expression: see section 12 13
radius
moderate moderate
regionalmoderate expression: see section 01 02
axial skeleton thoracic region
moderate moderate
regionalmoderate expression: see section 08 11 12 13 14 15 16 17 18 19
ulna
moderate moderate
regionalmoderate expression: see section 01 02
palatal shelf
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
humerus
moderate moderate
regionalmoderate expression: see section 01 02
fibula
moderate moderate
regionalmoderate expression: see section 04 05 06
cranium
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
clavicle
strong strong
regionalstrong expression: see section 08 09 16 17
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 18 19 21 moderate expression: see section 09 10 11 13 14 15 16 17 20
leg
moderate moderate
regionalmoderate expression: see section 01 02 03
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 16 17 18 19
nose
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
otic capsule
moderate moderate
regionalmoderate expression: see section 05 06 07 08 17 18 19
foot
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 17 18 19
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 08 11 12 13 14 15 16 17 18
tibia
moderate moderate
regionalmoderate expression: see section 04 05 06
femur
moderate moderate
regionalmoderate expression: see section 05 06 07 08
hand
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 17 18 19
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6162
Entity Detected:Kdelr2, ( MGI:1914163)
Sequence:sense strand is shown

>T6162
TCCCTGGCCACGTCGCGCCCGCTCCCGCCATCTTTCGCCGCTTCCTCTCTCCGGGGCTGCTGTCGCCGCG
TCCCGAGTCGCTGCCTAGCTCCGGTGCCGCTGTGCCGCGCCGACCGCCGCCGCCATGAACATCTTCCGGC
TGACTGGGGACCTGTCCCACCTGGCGGCCATCGTCATCCTACTGCTGAAGATCTGGAAGACGCGCTCCTG
TGCTGGGATCTCTGGGAAGAGTCAGCTCCTGTTCGCACTGGTCTTCACGACTCGCTACCTGGACCTTTTC
ACTTCCTTCATTTCCTTATACAACACGTCTATGAAGCTTATCTACATTGCCTGCTCCTATGCCACGGTGT
ACCTGATCTACATGAAATTTAAGGCCACCTATGATGGAAATCACGATACCTTCCGAGTGGAGTTCCTGGT
GGTTCCTGTAGGAGGCCTCTCATTTCTAGTCAATCATGACTTCTCTCCTCTTGAGATCTTATGGACCTTC
TCCATCTACCTGGAGTCCGTGGCCATCCTTCCACAGCTCTTTATGATCAGCAAGACTGGCGAGGCTGAGA
CCATCACCACCCACTACCTCTTCTTCCTGGGCCTCTACCGCGCTCTGTACCTGGTCAACTGGATCTGGCG
CTTCTACTTCGAGGGCTTCTTTGATCTCATTGCTGTGGTGGCTGGTGTCGTCCAGACCATTCTCTACTGC
GACTTCTTCTACTTGTACATTACAAAAGTACTCAAAGGGAAGAAGCTCAGCCTGCCAGCCTAAGTGCCAA
AGACCAGCAGCAGCCTCTGCCCTTCGGGGTGCTCGGACAGGGTCTCACCATGGCGAAGGCGAAGGCGAAG
GCTCGGGATGCTCAGTGGGCAGAAGCAGAGGCTTCTGCGGCAGCCACTCACTGGTGGCTCTTCGAGGATG
CAGAGGAAGAGACCAGGAGGCTTCTGTCTACTGCATCCTGCCTTATCTTTTTTATTACTATGTACAAAGA
TTTTTTTACACAAAGAAACTTAATGCTGTGTTAATAAATTCAGTATGTAGATGAATTGGGACAGTTTCCA
AAAGTGACGATTTTGTGCACAATAAGTACAAGCCTTTTTTATTTTTTGAGAATTTGTTGAGGTGGTCAAT
TCTTTGTGTCTACAATGAAATTATACTCCTTGACACTTGGTAGATTATCCAAACATCTGTCAAATTTCCA
TGCTATTTATTTTCTTTCTTTTCTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTGCCAAAAAGATAAGT
TGTGTTTGTTTGAAATCTGAGACATTGTGTTCCATTGGTGTTTCTGTCCAAAAGAGTCCTCGTTGTCCTG
GAAACCCTTCCTCAGATGTCACACTACATGTCAGGTTCGGGAGGATGACTAGAAAGTCCTAAGGTTTCAT
TACCAAAACTTGAAGGTTCCCAGCAGAAACTCAGCTGATGCTCACCAAACGCTCACCCTTCCCCCGCTGA
GGTGTGGCAGCTTGAGGTTATCCGAGGGTGCTGAGAGCTGCCACACCCAGAGGGTCTGAGCCACCTGCCC
TCTGACTATGGAGAGGGCTGGGGGTGATGGCTGTTTTGGATAGAGGACTCAGCTCCTCTGAGGAATGGAA
CACAAATAGCCACCTGTCACTGTCAGTCCGAAGGATCACTACAACCATGTTAGACTCAAATGTTTTTCTC
ATAGCACTTTTGGTGATAAAATGATTGACAATCTTTTTAATTGGCAACACCAGTTATCATTCCTTACATT
CTTTTTAGTATTTTTTTTAAACTGGGCTCTTGCATGACCTATATTGTGTTAACATCCTAAATAAACATTT
TATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3496891 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496891 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004155 same experiment
 EMAGE:31590 same embryo
 EMAGE:32248 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS