EMAGE mouse gene expression spatial database

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EMAGE:31662

Pvalb parvalbumin ( MGI:97821)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000449_11	euxassay_000449_01	euxassay_000449_02	euxassay_000449_03	euxassay_000449_04

euxassay_000449_05	euxassay_000449_06	euxassay_000449_07	euxassay_000449_08	euxassay_000449_09

euxassay_000449_10	euxassay_000449_12	euxassay_000449_13	euxassay_000449_14	euxassay_000449_15

euxassay_000449_16	euxassay_000449_17	euxassay_000449_18	euxassay_000449_19	euxassay_000449_20

euxassay_000449_21	euxassay_000449_22	euxassay_000449_23	euxassay_000449_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 19 weak expression: see section 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1778
Entity Detected:	Pvalb, parvalbumin ( MGI:97821)
Sequence:	sense strand is shown >T1778 TGGCCTCGAGCCAGATTCGGCACGAGGGCCCAGCTTTTCTGTATAGGCTCCGGCCTCTGCTCATCCAAGT TGCAGGATGTCGATGACAGACGTGCTCAGCGCTGAGGACATCAAGAAGGCGATAGGAGCCTTTGCTGCTG CAGACTCCTTCGACCACAAAAAGTTCTTCCAGATGGTGGGCCTGAAGAAAAAGAACCCGGATGAGGTGAA GAAGGTGTTCCATATTCTGGACAAAGACAAAAGTGGCTTCATTGAGGAGGATGAGCTGGGGTCCATTCTG AAGGGCTTCTCCTCAGATGCCAGAGACTTGTCTGCTAAAGAAACAAAGACGCTTCTGGCCGCTGGAGACA AGGATGGGGACGGCAAGATTGGGGTTGAAGAATTCTCCACTCTGGTGGCTGAAAGCTAAGTGGCGCTGAC TGCTTGGGTCCCCCACCTCTCCATCCCCAACGCCCCATCTCAGTCCTTCTCGCGGCCCTCCTGAGTTTCT GTTCAGTTTGTTTGTGTTATTTTTTACTCCCCCATCCTCTATGGCCCTCGGATGACG
Notes:	The probe template was PCR amplified from IMAGE:493697 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:493697 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000449 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source