EMAGE mouse gene expression spatial database

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EMAGE:31664

Npy ( MGI:97374)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000446_01	euxassay_000446_02	euxassay_000446_03	euxassay_000446_04	euxassay_000446_05

euxassay_000446_06	euxassay_000446_07	euxassay_000446_08	euxassay_000446_09	euxassay_000446_10

euxassay_000446_11	euxassay_000446_12	euxassay_000446_13	euxassay_000446_14	euxassay_000446_15

euxassay_000446_16	euxassay_000446_17	euxassay_000446_18	euxassay_000446_19	euxassay_000446_20

euxassay_000446_21	euxassay_000446_22	euxassay_000446_23	euxassay_000446_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

moderate

regional

moderate expression: see section 14 15 23 weak expression: see section 13

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 05 14 not examined expression: see section 22

medulla oblongata basal plate

moderate

regional

moderate expression: see section 22 weak expression: see section 06

diencephalon

moderate

regional

moderate expression: see section 08 12 14 23 weak expression: see section 13 not examined expression: see section 15

midbrain mantle layer

strong

regional

strong expression: see section 04 moderate expression: see section 14 weak expression: see section 05 15 16

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 08 09 11 12

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 05 06 08 11 12 13 not examined expression: see section 22

pons mantle layer

moderate

regional

moderate expression: see section 16 weak expression: see section 04 08 12 13 15 17 22 23

dorsal grey horn

moderate

regional

moderate expression: see section 06 07 08 09 10 11 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1751
Entity Detected:	Npy, ( MGI:97374)
Sequence:	sense strand is shown >T1751 TGGCCTCGAGCCAGATTCGGACGAGGCGCTCTCCGCATCCCACCGGTGGATCTCTTCTCTCACAGAGGCA CCCAGAGCAGAGCACCCGCCGCTCAGCGACGACTGCCCGCCCGCCACGATGCTAGGTAACAAGCGAATGG GGCTGTGTGGACTGACCCTCGCTCTATCTCTGCTCGTGTGTTTGGGCATTCTGGCTGAGGGGTACCCCTC CAAGCCGGACAATCCGGGCGAGGACGCGCCAGCAGAGGACATGGCCAGATACTACTCCGCTCTGCGACAC TACATCAATCTCATCACCAGACAGAGATATGGCAAGAGATCCAGCCCTGAGACACTGATTTCAGACCTCT AATGAAGGAAAGCACAGAAAACGCCCCCAGAACAAGGCTTGAAGACCCTTCCATGTGGTGATGGGAAATG AAACTTGTTCTCCCGACTTTTCCAAGTTTCCACCCTCATCTCATCTCATCCCCTGAAACCAGTCTGCCTG TCCCACCAATGCATGCCACCACTANGC
Notes:	The probe template was PCR amplified from IMAGE:482891 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:482891 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000446 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source