EMAGE mouse gene expression spatial database

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EMAGE:31684

Car12 carbonic anyhydrase 12 ( MGI:1923709)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000681_01	euxassay_000681_02	euxassay_000681_03	euxassay_000681_04	euxassay_000681_05

euxassay_000681_06	euxassay_000681_07	euxassay_000681_08	euxassay_000681_09	euxassay_000681_10

euxassay_000681_11	euxassay_000681_12	euxassay_000681_13	euxassay_000681_14	euxassay_000681_15

euxassay_000681_16	euxassay_000681_17	euxassay_000681_18	euxassay_000681_19	euxassay_000681_20

euxassay_000681_21	euxassay_000681_22	euxassay_000681_23	euxassay_000681_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 16 weak expression: see section 17 18 19

vibrissa

weak

regional

weak expression: see section 04 05 06 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3754
Entity Detected:	Car12, carbonic anyhydrase 12 ( MGI:1923709)
Sequence:	sense strand is shown >T3754 TGGCCTCGAGCCAGATTCGGCACGAGGGGGAGTTCTCTGATTTAGTCTCATTTTGACAGCATGGAATGTC CTCTTGAAGAACAAGGTTTATATATTTTTTTCCTTCTTCTATCCCTCCCTCCTTCTGACTTGATAAAAAT CTCTTCTGCTATCAGAATGTTTCATGGTGGCAGCTGTGTGTGCAGGATACGTGCATGCACACAAAACCTC TGACAGTCTTGGAGTCTGGGTGGCACCCTAGCCTTGGGACCAATGGCGTCACCACAGTAAGGACAAGCCT CAAGAAGAAGCAACACAATTTAGTTTCCTGAGCTTGGTCCAGGATTGTCGGCCAAGAGCTGGCATAAAGC AGTCCAGGGCAGCAAGACTGTCCTAAAGAAGTGTATACACTAACAAGTTATCTGAGAGTGCAGCGTGGTC ATCATAGCTGGCACGAGGACTGGTCAGAAAAGGAGAGGTGCTAGGAGGCAAAAACAGTGGTGTTTCTCCT TCCCTCTTCCCCTTCAGCACGGTCCAGCTATTCAGTGCTCGTTCAACCCTCATGGCTGAGACAAAACAAA GCC
Notes:	The probe template was PCR amplified from IMAGE:374725 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:374725 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000681 same experiment
	EMAGE:29668 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source