EMAGE mouse gene expression spatial database

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EMAGE:31689

Pdcd7 programmed cell death 7 ( MGI:1859170)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000698_01	euxassay_000698_02	euxassay_000698_03	euxassay_000698_04	euxassay_000698_05

euxassay_000698_06	euxassay_000698_07	euxassay_000698_08	euxassay_000698_09	euxassay_000698_10

euxassay_000698_11	euxassay_000698_12	euxassay_000698_13	euxassay_000698_14	euxassay_000698_15

euxassay_000698_16	euxassay_000698_17	euxassay_000698_18	euxassay_000698_19	euxassay_000698_20

euxassay_000698_21	euxassay_000698_22	euxassay_000698_23	euxassay_000698_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 17 18 19 20 21 22

lower jaw molar

moderate

regional

moderate expression: see section 08 weak expression: see section 19 20 21

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 17 18 19 20 21

upper jaw molar

moderate

regional

moderate expression: see section 08 09 weak expression: see section 21

vibrissa

weak

regional

weak expression: see section 05 06 07 08 23 24

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23

midbrain ventricular layer

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 12 13 18

lower jaw incisor

weak

regional

weak expression: see section 13 14 17 18

upper jaw incisor

weak

regional

weak expression: see section 13 14 17 18

chondrocranium

weak

regional

weak expression: see section 01 02 03 04 05 06 07 23 24

pons ventricular layer

weak

homogeneous

weak expression: see section 07 08 09 10 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3543
Entity Detected:	Pdcd7, programmed cell death 7 ( MGI:1859170)
Sequence:	sense strand is shown >T3543 TTCCAAAATGGTATTTATTCAGCTTTTAGGAAATTAAATAACAAAACTCAGAATAAGGCAAAACAAAAAC CATAACATGTCATATGACTGATCATGTCCAAATACAACAGCACTTATTAATGAATATAAAAATAAAATTT ATCTTTCATATTTGCTCTTTTCCCGGCTTTTATGACATGGATGTTTTTAGAAGCTTGAGCTATCCAGCCC CTACCATGTGACTGAGATTACAGTCCCACAGGGAAATAAGTTTCTATTCTTGGAGGCCCCACACAACTAG TTCCCTCACAACTCTTGCACTGGCACTTTCATGAGGAAAACAGGCACTGTCTGTCAGAGCACAAGCCACC CTGGCTCTGCTAACTCTTCAAGTTTTCTAAGCTCTCCACAGGACAACAGTTCTAAGCCATCAGTGATCCT GGCAGCCACTGCCCCATCATCTTCAGTAGCAGCCTCCCACACTGCACTGAAACTGGAAACTGTAGGTGCT CCTGGGAGCTGGAGGGCAGGTGGTATGG
Notes:	The probe template was PCR amplified from IMAGE:3166731 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166731 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000698 same experiment
	EMAGE:29208 same embryo
	EMAGE:29198 same embryo
	EMAGE:29336 same embryo
	EMAGE:30554 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source