EMAGE mouse gene expression spatial database

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EMAGE:31692

Lmo4 LIM domain only 4 ( MGI:109360)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004815_01	euxassay_004815_02	euxassay_004815_03	euxassay_004815_04	euxassay_004815_05

euxassay_004815_06	euxassay_004815_07	euxassay_004815_08	euxassay_004815_09	euxassay_004815_10

euxassay_004815_11	euxassay_004815_12	euxassay_004815_13	euxassay_004815_14	euxassay_004815_15

euxassay_004815_16	euxassay_004815_17	euxassay_004815_18	euxassay_004815_19	euxassay_004815_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 04 05 09 10 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 12 13 14 15

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 05 06 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 07 08

vibrissa

moderate

regional

moderate expression: see section 05 06 20 weak expression: see section 07 08 19

naris

moderate

regional

moderate expression: see section 11 12 14 15

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 16 17 18 weak expression: see section 07

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 09 10 11 14 15

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 08 09 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6509
Entity Detected:	Lmo4, LIM domain only 4 ( MGI:109360)
Sequence:	sense strand is shown >T6509 CTAGTTCTAGATCGCGGGCGGCCCCCTCCTCCCCTTTCCTGCTTCTGCGAGAACTCCCTCCCTCCCTCCA GCTCCGCCAGCCCCAGCGCCCCTGCCCTGGAAGCCGAGCGGCTCCGCTCGCATTTCGCCGCCGCCGCCGC CGCCGCCTCTCGCAGTGTTGCAATATAGGGGAGAAGCAGACCATGGTGAATCCGGGCAGCAGCTCGCAGC CGCCCCCGGTGACGGCCGGCTCCCTCTCCTGGAAGCGCTGCGCAGGCTGCGGGGGCAAGATCGCGGACCG CTTTCTGCTCTATGCCATGGACAGCTACTGGCATAGCCGCTGCCTCAAGTGCTCCTGCTGCCAGGCGCAG CTGGGCGACATCGGCACGTCCTGTTACACCAAGAGCGGCATGATCCTTTGCAGAAATGACTACATTAGGT TATTTGGGAATAGCGGTGCTTGCAGTGCCTGTGGACAGTCGATTCCTGCAAGTGAGCTCGTCATGAGGGC CCAAGGCAACGTGTATCATCTCAAGTGTTTCACATGTTCTACCTGCCGGAATCGCCTGGTCCCGGGAGAT CGGTTTCACTACATCAATGGCAGTTTATTTTGTGAACATGATAGACCCACAGCCCTCATCAATGGCCATT TGAATTCACTTCAGAGCAATCCACTACTGCCAGACCAGAAGGTCTGCTGAGAGGTCAGAGTAATGCAGAA TGCGTGCCTTCATCTCAGAATTGCTCATCCCAGGTGGATCCCATGTGTCTGCAGTAGACCAGTCACCTTT GTAGCCAGCACCATGCCATCGCGCCCCCTTTAGTCTTGAGTACCCTTCCTGCATTTATTGGTGTATTAAA ATGACTGAATATGAACATTAAGGACTCCATGAACCTGGGCTAATGGGAGATGGTAGCGAGGATGAAAAAA GATCCACCGAAGGACATCTAGGGGGGAGGGTGCTTGGGGGGGGGGGGGAAATGACTAATGAAGCTAATTA AACGAAGCATCACCAATCTGCTTTCTACCCTCATTAACAATTAGCAGGGCCCCGGCCAGAGTGTGAGCCC TGTTTTACTTTAACAACATTCTGTTTGCTCTTTGTATATTTAAGTGTTGTAATGAAATGTGTTTCAATCA ACCTGAACATGAGTTAAAAGGAAAGAGATGTGGCTTTTGTGATAATTCTATCACAAACACTTTTATTGTA TCTCTGTAAAATACAATGTATGTATGCATGTGAGTGTTCTTGTCCTGATGTTGCTCCGCCCATGACAAAG GTTAAAAAAAAAGAAAAGAAGAAAAAAAAAATGAGGCTCATAGCCGCTACTATGTAGAAATCCCCCCTAC TTCTAATTTGCTGAATGAAGAAAAAAAAATCTTTTATTTGTGATATTTTCAGAGACATTTGCTCTAGTAT GGTGTATTTAAATAATAAAAACTTAAAAGAAAAAATATTCAACAGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589532 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589532 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004815 same experiment
	EMAGE:31617 same embryo
	EMAGE:31702 same embryo
	EMAGE:29645 same embryo
	EMAGE:31700 same embryo
	EMAGE:31688 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source